2022
DOI: 10.1002/adhm.202100805
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Designer Extracellular Vesicles Modulate Pro‐Neuronal Cell Responses and Improve Intracranial Retention

Abstract: Gene/oligonucleotide therapies have emerged as a promising strategy for the treatment of different neurological conditions. However, current methodologies for the delivery of neurogenic/neurotrophic cargo to brain and nerve tissue are fraught with caveats, including reliance on viral vectors, potential toxicity, and immune/inflammatory responses. Moreover, delivery to the central nervous system is further compounded by the low permeability of the blood brain barrier. Extracellular vesicles (EVs) have emerged a… Show more

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Cited by 10 publications
(18 citation statements)
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“…istics and display tropism for particular cells or tissues, [78,79] they can also be modified with ligands (e.g., integrins, protein ligands, peptides, nucleic acids) to facilitate the targeting/recognition of specific receptors for preferential binding and payload delivery, avoiding major off-target effects. [43,[80][81][82] Type II pneumocytes constitute 60% of alveolar epithelial cells and make up 10-15% of all cells present in the lungs. [83] The relevance of this is that alveolar epithelial cells are the first line of cells that secrete proinflammatory mediators (e.g., cytokines, chemokines) in response to pathogenic/harmful stimuli, leading and orchestrating the recruitment of innate and adaptative immune cells to the site of injury and/or infection.…”
Section: Discussionmentioning
confidence: 99%
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“…istics and display tropism for particular cells or tissues, [78,79] they can also be modified with ligands (e.g., integrins, protein ligands, peptides, nucleic acids) to facilitate the targeting/recognition of specific receptors for preferential binding and payload delivery, avoiding major off-target effects. [43,[80][81][82] Type II pneumocytes constitute 60% of alveolar epithelial cells and make up 10-15% of all cells present in the lungs. [83] The relevance of this is that alveolar epithelial cells are the first line of cells that secrete proinflammatory mediators (e.g., cytokines, chemokines) in response to pathogenic/harmful stimuli, leading and orchestrating the recruitment of innate and adaptative immune cells to the site of injury and/or infection.…”
Section: Discussionmentioning
confidence: 99%
“…EV Isolation and Characterization: eEVs were isolated from culture media 24 h after transfection of the donor cells, as previously reported. [43,46,47] The culture media was centrifuged at 2000g for 30 min at 4 °C to remove dead cells and debris. After centrifugation, Total exosome isolation reagent (Thermo Fisher Scientific, 44-783-59) was added to the supernatant containing the cell-free culture media following manufacturer instructions.…”
Section: Methodsmentioning
confidence: 99%
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“…[7][8][9][10] Additionally, our lab and others have shown their significant potential to serve as efficient nanocarriers for an extensive array of therapeutic applications, including regenerative medicine and cancer. [7,[11][12][13][14][15][16][17][18][19][20] Ischemic disorders and tissue injury often result in deficient vascularization, which can lead to infections, inflammation, and impaired tissue regeneration (e.g., non-healing ulcers, partial necrosis). [21,22] Cell therapies, involving the use of vascular progenitor cells or induced endothelial cells (iECs), have emerged as a powerful approach to increase neovascularization and promote tissue repair and regeneration under multiple conditions, including myocardial ischemia, limb ischemia, and stroke, among others.…”
Section: Introductionmentioning
confidence: 99%
“…[ 7–10 ] Additionally, our lab and others have shown their significant potential to serve as efficient nanocarriers for an extensive array of therapeutic applications, including regenerative medicine and cancer. [ 7,11–20 ]…”
Section: Introductionmentioning
confidence: 99%