Designer DNA nanostructures for therapeutics

Jiang, Shuoxing; Ge, Zhilei; Mou, Shan; Yan, Hao; Fan, Chunhai

doi:10.1016/j.chempr.2020.10.025

Cited by 100 publications

(93 citation statements)

References 174 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of the exciting opportunities in virus-DNA hybrids research are still unexplored, and several challenges concerning optimization for physiological conditions remain to be solved, especially in case of DNA nanostructures [61,62]. The most prominent of these are stability at low-cation conditions, resistance against nucleases, low pharmacokinetic availability, low cell uptake, possible inflammatory response and accurate loading and release of drug molecules [23,24,46,52,[61][62][63][64][65][66][67][68][69][70][71]. Outstanding issues in virus-based nanocarriers are related to unknown adverse effects caused by the adaptive immunity against the viral proteins [72].…”

Section: Discussionmentioning

confidence: 99%

“…Besides the discrete objects, well-ordered DNA origami-based 2D lattices can reach~10 cm 2 surface areas [20] and 3D DNA crystals millimeter scale dimensions [21,22]. So far, these versatile DNA nanoshapes have found applications in biomedicine, diagnostics and therapeutics [23,24], nanofabrication [25,26], molecular electronics [27,28] and super-resolution imaging [29], and as nanorulers [30], plasmonic or photonic apparatuses [31,32], precise nanoscopic measurement tools [33], tunable nanopores [34,35] and dynamic/robotic devices [36,37].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hybrid Nanoassemblies from Viruses and DNA Nanostructures

et al. 2021

View full text Add to dashboard Cite

Viruses are among the most intriguing nanostructures found in nature. Their atomically precise shapes and unique biological properties, especially in protecting and transferring genetic information, have enabled a plethora of biomedical applications. On the other hand, structural DNA nanotechnology has recently emerged as a highly useful tool to create programmable nanoscale structures. They can be extended to user defined devices to exhibit a wide range of static, as well as dynamic functions. In this review, we feature the recent development of virus-DNA hybrid materials. Such structures exhibit the best features of both worlds by combining the biological properties of viruses with the highly controlled assembly properties of DNA. We present how the DNA shapes can act as “structured” genomic material and direct the formation of virus capsid proteins or be encapsulated inside symmetrical capsids. Tobacco mosaic virus-DNA hybrids are discussed as the examples of dynamic systems and directed formation of conjugates. Finally, we highlight virus-mimicking approaches based on lipid- and protein-coated DNA structures that may elicit enhanced stability, immunocompatibility and delivery properties. This development also paves the way for DNA-based vaccines as the programmable nano-objects can be used for controlling immune cell activation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hybrid Nanoassemblies from Viruses and DNA Nanostructures

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Due to their unique properties, DNA nanostructures have great potential to be broadly applied in biotechnology and biomedicine. For example, some DNA nanostructures can penetrate cell membranes without transfection agents for intracellular detection or cargo transport, which provides alternative methods for molecular diagnosis and drug delivery (Jiang et al, 2021). Fan group demonstrated that DNA tetrahedron modified with unmethylated CpG motifs can noninvasively enter macrophage-like cells without transfection agents and induce the secretion of cytokines including tumor necrosis factor-α, interleukin-6 and interleukin-12 (Li et al, 2011).…”

Section: Application Of Dna Framework In the Construction Of Nanostructuresmentioning

confidence: 99%

Application of Nucleic Acid Frameworks in the Construction of Nanostructures and Cascade Biocatalysts: Recent Progress and Perspective

Zhu

Song

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Nucleic acids underlie the storage and retrieval of genetic information literally in all living organisms, and also provide us excellent materials for making artificial nanostructures and scaffolds for constructing multi-enzyme systems with outstanding performance in catalyzing various cascade reactions, due to their highly diverse and yet controllable structures, which are well determined by their sequences. The introduction of unnatural moieties into nucleic acids dramatically increased the diversity of sequences, structures, and properties of the nucleic acids, which undoubtedly expanded the toolbox for making nanomaterials and scaffolds of multi-enzyme systems. In this article, we first introduce the molecular structures and properties of nucleic acids and their unnatural derivatives. Then we summarized representative artificial nanomaterials made of nucleic acids, as well as their properties, functions, and application. We next review recent progress on constructing multi-enzyme systems with nucleic acid structures as scaffolds for cascade biocatalyst. Finally, we discuss the future direction of applying nucleic acid frameworks in the construction of nanomaterials and multi-enzyme molecular machines, with the potential contribution that unnatural nucleic acids may make to this field highlighted.

show abstract

“…Nucleic acid nanostructures can be used not only for diagnosis and treatment but also for sensing, and precise programmable nucleic acid nanostructures are used for single‐molecule nanopore detection. [ 43‐44 ] In addition, we recommend the recently published research progress of single‐molecule solid‐state nanopores. [ 45 ]…”

Section: Analysis Of Single‐entitymentioning

confidence: 99%

Electrochemical Analysis for Multiscale Single Entities on the Confined Interface^†

Zhao

Zuo

et al. 2021

Chin. J. Chem.

View full text Add to dashboard Cite

Electrochemistry | Sensors | Single-molecule studies| Single-particle studies | Single-cells studies Single entities detection reveals heterogeneity and random processes hidden in ensemble measurements. Obtaining accurate single-entity information is challenging. The electrochemical analysis is at high spatial resolution and high temporal resolution to analyze single entities and measure the fast kinetics process. In this minireview, we will focus on the electrochemical strategies for single multiscale entities.Haipei Zhao received her master's degree from the Shanghai University in 2019. Now, she is a Ph.D. candidate at the Shanghai Jiao Tong University. She focuses on the research of electrochemical strategies for cancer diagnosis. (left).

show abstract

Designer DNA nanostructures for therapeutics

Cited by 100 publications

References 174 publications

Hybrid Nanoassemblies from Viruses and DNA Nanostructures

Hybrid Nanoassemblies from Viruses and DNA Nanostructures

Application of Nucleic Acid Frameworks in the Construction of Nanostructures and Cascade Biocatalysts: Recent Progress and Perspective

Electrochemical Analysis for Multiscale Single Entities on the Confined Interface^†

Contact Info

Product

Resources

About

Designer DNA nanostructures for therapeutics

Cited by 100 publications

References 174 publications

Hybrid Nanoassemblies from Viruses and DNA Nanostructures

Hybrid Nanoassemblies from Viruses and DNA Nanostructures

Application of Nucleic Acid Frameworks in the Construction of Nanostructures and Cascade Biocatalysts: Recent Progress and Perspective

Electrochemical Analysis for Multiscale Single Entities on the Confined Interface†

Contact Info

Product

Resources

About

Electrochemical Analysis for Multiscale Single Entities on the Confined Interface^†