Designer D-form self-assembling peptide nanofiber scaffolds for 3-dimensional cell cultures

Luo, Zhongli; Yue, Yuanyuan; Zhang, Yanfang; Yuan, Xingliang; Gong, Jianping; Wang, Liangliang; He, Bin; Liu, Zhong; Sun, Yan; Liu, Jiao; Hu, Mingfeng; Zheng, Jie

doi:10.1016/j.biomaterials.2013.03.081

Cited by 77 publications

(61 citation statements)

References 50 publications

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“…Finally, our study suggests that the chirality of peptides does not interfere with the molecular self-assembly nor the formation of well-defined nano-structures. This is in accordance with the findings of Luo et al (42), who evaluated the chiral peptides of EAK16.…”

Section: Discussionsupporting

confidence: 93%

“…This study indicated that D-RADA16 scaffolds resisted the enzymatic hydrolysis catalyzed and exhibited high biostability compared to its L-form enantiomer. This agrees with the findings of previous studies that peptide synthesized from D-amino acid can be exceptionally effective in increasing the resistance towards proteolysis (29,30,32,42,53). …”

Section: Discussionsupporting

confidence: 93%

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Designer D-form self-assembling peptide scaffolds promote the proliferation and migration of rat bone marrow-derived mesenchymal stem cells

Chen

Zhou

et al. 2017

International Journal of Molecular Medicine

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Self-assembling peptide (SAP) nanofiber hydrogel scaffolds have become increasingly important in tissue engineering due to their outstanding bioactivity and biodegradability. However, there is an initial concern on their long-term clinical use, since SAPs made of L-form amino acid sequences are sensitive to enzymatic degradation. In this study, we present a designer SAP, D-RADA16, made of all D-amino acid. We investigated the nanofiber morphology of D-RADA16, its potential for the culture of bone marrow-derived mesenchymal stem cells (BMSCs), and the proteolytic resistance of the biomaterial. The results revealed that D-RADA16 exhibited stable β-sheets and formed interwoven nanofiber scaffolds in water. D-RADA16 and L-RADA16 hydrogel scaffolds were both found to promote the proliferation and migration of rat BMSCs in the 3D cell culture microenvironment. Furthermore, the D-RADA16 scaffolds exhibited a higher proteolytic resistance against proteinase K than the L-RADA16 scaffolds. These observations indicate that D-RADA16 hydrogel scaffolds have excellent bioactivity, biocompatibility and biostability, and thus may serve as promising candidates for long-term application in vivo.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Designer D-form self-assembling peptide scaffolds promote the proliferation and migration of rat bone marrow-derived mesenchymal stem cells

Chen

Zhou

et al. 2017

International Journal of Molecular Medicine

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“…On the other hand, other examples were reported of the L-enantiomer in vitro having lower cytotoxicity than either its D-counterpart or the racemic mixture [53]. Finally, there are also cases of identical performance for either enantiomer used as 3D-scaffold for cell-culture cytotoxicity assays in vitro [54]. …”

Section: Biomaterials Use and Cytotoxicitymentioning

confidence: 99%

The Unexpected Advantages of Using D-Amino Acids for Peptide Self- Assembly into Nanostructured Hydrogels for Medicine

Melchionna¹,

Styan²,

Marchesan

2016

CTMC

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Self-assembled peptide hydrogels have brought innovation to the medicinal field, not only as responsive biomaterials but also as nanostructured therapeutic agents or as smart drug delivery systems. D-amino acids are typically introduced to increase the peptide enzymatic stability. However, there are several reports of unexpected effects on peptide conformation, self-assembly behavior, cytotoxicity and even therapeutic activity. This mini-review discusses all the surprising twists of heterochiral self-assembled peptide hydrogels, and delineates emerging key findings to exploit all the benefits of D-amino acids in this novel medicinal area.

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“…Their water adsorbing ability is due to the hydrophilic groups present in their structure. The organic pollutants get adsorb into hydrogels as the ionic groups of organic pollutants form physical or chemical interaction with hydrophilic groups of hydrogels [5][6][7][8][9][10]. The addition of reinforcing agents and using crosslinkers during their synthesis helps in increasing the mechanical strength of the hydrogels.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of a nanocomposite hydrogel for combined photocatalytic degradation of a mixture of malachite green and fast green dye

Sharma

ALOthman

Kumar

et al. 2017

Nanotechnol. Environ. Eng.

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In this study, starch/poly(alginic acid-cl-acrylamide)/Fe/Zn nanocomposite hydrogel (ST/PL(AA-clAAm)/Fe/Zn NCHG) was synthesized by polymerization/ co-precipitation method. This nanocomposite hydrogel was prepared with the notion to remove the mixture of organic pollutants from the water system. Mixture of malachite green and fast green dye was used to check the photocatalytic degradation ability of the prepared nanocomposite hydrogel. ST/PL(AA-cl-AAm)/Fe/Zn NCHG was characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy and Fourier transform infrared. The degradation of the two dyes followed the pseudo-first-order kinetics. The results showed that by using the prepared nanocomposite hydrogel, 91% of malachite green and 82% of fast green dye, respectively, were degraded within 5 h of their contact.

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Designer D-form self-assembling peptide nanofiber scaffolds for 3-dimensional cell cultures

Cited by 77 publications

References 50 publications

Designer D-form self-assembling peptide scaffolds promote the proliferation and migration of rat bone marrow-derived mesenchymal stem cells

Designer D-form self-assembling peptide scaffolds promote the proliferation and migration of rat bone marrow-derived mesenchymal stem cells

The Unexpected Advantages of Using D-Amino Acids for Peptide Self- Assembly into Nanostructured Hydrogels for Medicine

Fabrication and characterization of a nanocomposite hydrogel for combined photocatalytic degradation of a mixture of malachite green and fast green dye

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