Designed Three-Dimensional Collagen Scaffolds for Skin Tissue Regeneration

Ahn, Sang-Ho; Yoon, Hun‐Young; Kim, GeunHyung; Kim, Yunyoung; Lee, Su-Hee; Chun, Wook

doi:10.1089/ten.tec.2009.0511

Cited by 77 publications

(54 citation statements)

References 23 publications

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“…The basic components for tissue engineering involve an interactive triad of scaffolds [79][80][81] , signaling molecules [82][83][84] and cells [24,33,41,85] , which play a fundamental role in the regeneration process [24,76,86,87] . Scaffolds serve as a threedimensional template mimicking the extracellular matrix; signaling molecules enhance this cellular activity by stimulating cells to migrate, proliferate and differentiate; and cells provide the machinery synthesis of the extracellular matrix and tissue regeneration [24,75,76,88,89] .…”

Section: Perspectives On Tdscs In Regenerative Approachesmentioning

confidence: 99%

Tooth-derived stem cells: Update and perspectives

Saito¹,

Silvério²,

Casati³

et al. 2015

WJSC

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Section: Perspectives On Tdscs In Regenerative Approachesmentioning

confidence: 99%

Tooth-derived stem cells: Update and perspectives

Saito¹,

Silvério²,

Casati³

et al. 2015

WJSC

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“…6 Three-dimensional (3D) collagen scaffolds seeded with fibroblasts and keratinocytes can generate a stratum corneum similar to that found in human skin. 7 In addition to fabricated tissue, collagen-based matrices have also been increasingly utilized to control the differentiation and orientation of various cell populations in vitro and in vivo. Micro patterned collagen has been used to direct bone formation, manage smooth muscle cell morphology, and to control mesenchymal stem cell attachment.…”

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confidence: 99%

Ultrarapid Purification of Collagen Type I for Tissue Engineering Applications

Pacak

Powers

Cowan

2011

Tissue Engineering Part C: Methods

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Acid-solubilized collagen type I (COL1) can form highly organized, three-dimensional scaffolds for a wide variety of bioengineering and cell culture applications. A rapid COL1 isolation method would be a valuable tool for both basic and translational researchers because conventional techniques require days or weeks to complete, typically use nonhuman animal tissues as a source material, and do not efficiently purify autologous COL1 from small samples. Here, we describe a 3-h method to isolate COL1 from rabbit, lamb, and human skin in sufficient quantities for fabrication of autologously derived tissues by using a rapid agitation technique and incorporating centrifugal filtration units into a traditional isolation procedure. We demonstrate that the purified product is comparable to traditional preparations using polyacrylamide gel electrophoresis, transmission electron microscopy, and collagen content assays. In addition, our COL1 is able to support myogenic cell growth and direct orientation of these cells in vitro. Importantly, this ultrarapid COL1 isolation procedure increases the feasibility of autologous COL1 use in humans as well as overall safety for clinical patients.

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“…Mientras que los resultados arrojados en esta investigación, muestran que el diámetro de poro promedio de las matrices de 2:8, AH y Col entrecruzadas con 50 mM EDC.HCl se encuentra alrededor de los 50 µm; la bibliografía sugiere que para matrices porosas con aplicaciones en sustitutos de piel, el rango del tamaño de poro ideal debería oscilar entre los 100 µm y 200 µm [20,21]. Por lo tanto, es necesario hacer más pruebas en la fabricación de las matrices con variaciones tanto en la temperatura y tiempo de congelación de la muestra como en los tiempos de liofilización para obtener matrices con un tamaño de poro ideal para este tipo de aplicaciones.…”

Section: Evaluación De Viabilidad Celular Sobre Matrices: Prueba De Mttunclassified

Construcción de matrices como sustitutos dérmicos: aplicación potencial en la regeneración de piel (MATRIX CONSTRUCTION AS DERMAL SUBSTITUTES: POTENTIAL APPLICATION IN SKIN REGENERATION)

Serna

Molina

2014

RIB

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Resumen ─ La pérdida de grandes porciones de piel limita su capacidad de regeneración, haciendo difícil su tratamiento. Mediante la ingeniería de tejidos se han desarrollado sustitutos dérmicos que promueven la regeneración; sin embargo, es necesario hallar un material para el crecimiento de fibroblastos que sea un sustituto dérmico adecuado. En este trabajo se construyeron matrices tridimensionales porosas de colágeno y colágeno-ácido hialurónico evaluando su potencial como sustituto dérmico. Las matrices se entrecruzaron con 1-etil-3-(3-dimetilaminopropil) carbodiimida hidrocloruro. Se evaluó la porosidad, se realizaron pruebas de degradación, evaluación de adherencia celular con fibroblastos humanos y, ensayos de viabilidad y proliferación. Las matrices presentaron poros de 50 µm de diámetro uniformemente distribuidos. Las matrices de colágeno y colágeno-ácido hialurónico (2:8 y 4:6) presentaron una degradación progresiva con pesos similares. Luego de 24 horas en cultivo, los resultados de la prueba de MTT mostraron que los fibroblastos en colágeno proliferaron; mientras que los fibroblastos en colágeno-ácido hialurónico no evidenciaron proliferación. Aunque la estructura porosa y la tasa de degradación de las matrices son similares, las de colágeno brindan mejores condiciones de adhesión y proliferación a los fibroblastos cultivados en comparación con las que tienen ácido hialurónico como adición, convirtiéndose en el mejor andamio para ser empleado como sustituto dérmico entre las matrices evaluadas. Palabras clave ─ Ácido hialurónico, colágeno, fibroblastos, matriz extracelular.Abstract ─ Wounds can cause loss of skin that limits the skin regeneration, making the treatment more difficult to address. The tissue engineering has developed skin substitutes that promote skin regeneration; however, it is imperative to find materials that allow fibroblast growth in order to find an appropriate skin substitute. The construction of tridimensional porous collagen and collagenhyaluronic acid matrixes crosslinked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride is presented in order to evaluate their potential use in skin regeneration therapies. Matrix porosity was evaluated. Degradation tests, human fibroblast adherence, viability and proliferation tests were performed. Matrixes had a uniform distributed porosity with mean diameters of 50 µm. Both collagen and collagen-hyaluronic acid (2:8 and 4:6) matrixes presented a progressive degradation rate with similar weight. After 24 hours under culture, the number of fibroblasts seeded on collagen matrixes were doubled, while the number of fibroblast seeded on collagen-hyaluronic acid matrixes remained similar. Even though porous structure and degradation rate of different types of constructed matrixes in this study are similar, collagen matrixes offer better adhesion and proliferation conditions for seeded fibroblasts in comparison with hyaluronic acid added matrixes, making them the best scaffold to be employed as a dermic substitute among the matrixes ...

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Designed Three-Dimensional Collagen Scaffolds for Skin Tissue Regeneration

Cited by 77 publications

References 23 publications

Tooth-derived stem cells: Update and perspectives

Tooth-derived stem cells: Update and perspectives

Ultrarapid Purification of Collagen Type I for Tissue Engineering Applications

Construcción de matrices como sustitutos dérmicos: aplicación potencial en la regeneración de piel (MATRIX CONSTRUCTION AS DERMAL SUBSTITUTES: POTENTIAL APPLICATION IN SKIN REGENERATION)

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