Designations F18ab and F18ac for the related fimbrial types F107, 2134P and 8813 of Escherichia coli isolated from porcine postweaning diarrhoea and from oedema disease

Rippinger, P.; Bertschinger, H. U.; Imberechts, Hein; Nagy, Béla; Sorg, I.; Stamm, M.; Wild, P.; Wittig, W

doi:10.1016/0378-1135(94)00141-i

Cited by 83 publications

(66 citation statements)

References 23 publications

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“…F18ab is usually found on STEC, ETEC or ETEC/STEC and F18ac is usually expressed by ETEC (Fairbrother et al, 2005). This typically produces STa and STb toxins, sometimes associated with Stx2e toxin (Rippinger et al, 1995;Francis, 2002).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

Toledo

Gómez

Cruz

et al. 2012

Journal of Medical Microbiology

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Faecal Escherichia coli isolates from suckling (n5503) and weaning (n5450) piglets with and without diarrhoea from 10 farms in Mexico were examined for identification and prevalence of virulence genes. E. coli isolates were tested further for enterotoxin (LT, STa, STb, Stx1, Stx2 and EAST1), fimbrial (F4, F5, F6, F17, F18 and F41) and eae adhesin genes by multiplex PCR. Of the 953 isolates of E. coli examined by multiplex PCR, 650 (68.2 %) isolates were positive for at least one adhesin gene. Among the isolates from diarrhoeic piglets, F41 (72 %) was the most prevalent adhesin followed by eae adhesin (27 %), F6 (12 %), F17 (9 %), F18 (9 %), F5 (8 %) and F4 (3 %). Enterotoxin genes were detected in 424 (44.5 %) of the isolates, of which EAST1 (38 %) and STa (30 %) were the most common, followed by STb (17 %), Stx2 (6 %) and LT (5 %). Twenty-three per cent of isolates from suckling piglets and 43 % of isolates from weaned piglets carried both enterotoxin and adhesin genes, the most common virotypes being F41 : STa, F41 : EAST1, EAST1 : eae, F41 : F6, F41 : STa : STb, F41 : eae and F17 : eae. The present study examined for the first time, to our knowledge, the prevalence of 13 virulence genes among E. coli strains isolated from piglets with and without diarrhoea in Mexico. The results suggested that there are a wide variety of virulence genes associated with diarrhoea in piglets. This study provides baseline information on the significance of specific virotypes associated with suckling and weaning periods in piglets in Mexico.

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Section: Introductionmentioning

confidence: 99%

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

Toledo

Gómez

Cruz

et al. 2012

Journal of Medical Microbiology

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“…The magnitude of challenge used in this study did not result in mortality which may be related to the degree of pathogenicity of the strain used. In this experiment, we used a strain that was initially isolated from the field [9]. We did observe frank diarrhea with varying degrees of severity in about half of the susceptible pigs used in initial challenge testing (data not shown) and in the control group.…”

Section: Discussionmentioning

confidence: 99%

“…Enterotoxigenic E. coli have been associated with diarrhea in neonatal and postweaned piglets and its fimbriae have been recognized as colonization factors [7]. Aside from colonization factors K88, K99, and 987P which have been associated with neonatal enteric colibacillosis, the recently characterized colonization factors F18ab (formerly F107) [1] and F18ac (formerly 2134P, Av24, 8199, or 8813) [4,[8][9][10] have also been associated with postweaning diarrhea and edema disease in pigs. In a study in Switzerland [9], the relatedness of the fimbriae F18ab and F18ac was confirmed by polymerase chain reaction (PCR) and serological methods (nomenclature used was F107 for F18ab and 2134P or 8813 for F18ac).…”

Section: Methodsmentioning

confidence: 99%

“…Bacteria and cultivation conditions: E. coli strain 8199 (O141ab: H4: F18ac + : STIa, STII) [9] was obtained from Prof. Bertschinger, Institute of Veterinary Bacteriology, University of Zürich, Switzerland. The bacteria were grown on Iso-Sensitest Agar (Oxoid, Unipath Ltd., Hampshire, England) containing the dye Alizarin yellow R (Sigma, MO, U.S.A.), 0.0625% w/v for 20 hr at 37°C in an atmosphere containing 5% CO 2 [11].…”

Section: Methodsmentioning

confidence: 99%

“…Aside from colonization factors K88, K99, and 987P which have been associated with neonatal enteric colibacillosis, the recently characterized colonization factors F18ab (formerly F107) [1] and F18ac (formerly 2134P, Av24, 8199, or 8813) [4,[8][9][10] have also been associated with postweaning diarrhea and edema disease in pigs. In a study in Switzerland [9], the relatedness of the fimbriae F18ab and F18ac was confirmed by polymerase chain reaction (PCR) and serological methods (nomenclature used was F107 for F18ab and 2134P or 8813 for F18ac). Independent studies in Australia [2] and Sweden [5] showed a prevalence rate of 62% and 30% based on hybridization test using F18 specific oligonucleotide probes thereby illustrating the epidemiological importance of this E. coli phenotype in pigs.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Oral Egg Antibody in Experimental F18+ Escherichia coli Infection in Weaned Pigs.

et al. 1997

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ABSTRACT. The protection conferred by egg antibody specific for F18-fimbriae against infection with F18 + Escherichia coli was studied in controlled passive immunization trials involving weaned pigs. Parameters of protection consisted of body weight gain, frequency and severity of diarrhea and recovery of the challenge strain of F18 + E. coli. Weaned pigs at four weeks of age were challenge exposed once daily for three days by oral inoculation with 10 11 cfu of virulent F18 + E. coli followed by daily administration of antibody supplemented feed for 9 days starting from the first challenge day 0. Results showed a dose-dependent response to antibody treatment. The group of pigs given 1:50 titer of antibody in feed had less frequency of diarrhea (P<0.01-0.05), higher rate of gain (P<0.01) and lower isolation rate of challenge strain in rectal and intestinal swabs (P<0.01) compared to non-treated control. In the same manner, the anti-F18 antibody significantly reduced adherence of F18 + E. coli to pig intestinal epithelial cells in vitro (P<0.01). Results suggest that egg antibodies specific for the F18 fimbriae is a suitable immunotherapeutic agent for pigs infected with F18 + E. coli and that pigs can be protected from overt clinical disease and the subsequent reduced performance arising from infection with this pathogen. -KEY WORDS : egg antibody, Escherichia coli, F18 fimbriae, passive immunity, porcine postweaning diarrhea.J. Vet. Med. Sci. 59(10): 917-921, 1997 passive immunity with anti-fimbrial antibodies essentially prevents bacterial adherence by interfering with the surface localized fimbriae from adhering to intestinal receptors. The net effect is reduced extent and intensity of colonization precluding the overt expression of clinical disease. Recently, we have shown that the use of orally administered egg antibodies directed against the K88, K99, and 987P fimbriae of E. coli effectively prevented diarrhea in neonatal isolated pigs [12]. In this report, we extend this study by using egg antibodies directed against the F18 fimbriae involving weaned pigs. MATERIALS AND METHODSBacteria and cultivation conditions: E. coli strain 8199 (O141ab: H4: F18ac + : STIa, STII) [9] was obtained from Prof. Bertschinger, Institute of Veterinary Bacteriology, University of Zürich, Switzerland. The bacteria were grown on Iso-Sensitest Agar (Oxoid, Unipath Ltd., Hampshire, England) containing the dye Alizarin yellow R (Sigma, MO, U.S.A.), 0.0625% w/v for 20 hr at 37°C in an atmosphere containing 5% CO 2 [11]. The bacteria were suspended in phosphate-buffered saline (PBS; pH 7.2), the fimbriae detached by incubating the suspension in a water bath at 60°C for 20 min with shaking (50 rpm), and the bacterial cells removed by centrifugation for 20 min at 12,000 × g, 4°C. The fimbriae were precipitated by adding 20% v/v of saturated ammonium sulfate followed by centrifugation for 20 min at 12,000 × g. The pellet was resuspended in 0.1 M Tris-HCl buffer (pH 8.8) and dialyzed against the same buffer. The suspension was applied...

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Structural Analysis of F18 Fimbriae Expressed by Porcine Toxigenic Escherichia coli

Hahn

Wild

Schraner

et al. 2000

Journal of Structural Biology

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Designations F18ab and F18ac for the related fimbrial types F107, 2134P and 8813 of Escherichia coli isolated from porcine postweaning diarrhoea and from oedema disease

Cited by 83 publications

References 23 publications

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

Effect of Oral Egg Antibody in Experimental F18+ Escherichia coli Infection in Weaned Pigs.

Structural Analysis of F18 Fimbriae Expressed by Porcine Toxigenic Escherichia coli

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