Design, synthesis and characterization of enzyme-analogue-built polymer catalysts as artificial hydrolases

Mathew, Dona; Thomas, Benny; Devaky, K. S.

doi:10.1080/21691401.2019.1576703

Cited by 19 publications

(12 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Major advantages are related to the fact that the building blocks of peptides are amino acids that work as catalytic groups, assemble, and form supramolecular structures through noncovalent interactions, all traits of natural enzymes [ 73 ]. Based on this approach, many artificial enzymes have been constructed, such as hydrolases, aldolase, oxidoreductase, among others [ 73 , 74 , 75 ]. As for other synthetic alternatives, lower catalytic activity and substrate specificity are still challenging, and molecular imprinting can contribute to improving those features.…”

Section: Molecular Imprinting Technologymentioning

confidence: 99%

Molecular Imprinting on Nanozymes for Sensing Applications

et al. 2021

View full text Add to dashboard Cite

As part of the biomimetic enzyme field, nanomaterial-based artificial enzymes, or nanozymes, have been recognized as highly stable and low-cost alternatives to their natural counterparts. The discovery of enzyme-like activities in nanomaterials triggered a broad range of designs with various composition, size, and shape. An overview of the properties of nanozymes is given, including some examples of enzyme mimics for multiple biosensing approaches. The limitations of nanozymes regarding lack of selectivity and low catalytic efficiency may be surpassed by their easy surface modification, and it is possible to tune specific properties. From this perspective, molecularly imprinted polymers have been successfully combined with nanozymes as biomimetic receptors conferring selectivity and improving catalytic performance. Compelling works on constructing imprinted polymer layers on nanozymes to achieve enhanced catalytic efficiency and selective recognition, requisites for broad implementation in biosensing devices, are reviewed. Multimodal biomimetic enzyme-like biosensing platforms can offer additional advantages concerning responsiveness to different microenvironments and external stimuli. Ultimately, progress in biomimetic imprinted nanozymes may open new horizons in a wide range of biosensing applications.

show abstract

Section: Molecular Imprinting Technologymentioning

confidence: 99%

Molecular Imprinting on Nanozymes for Sensing Applications

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Molecularly imprinted polymers (MIPs) are smart synthetic materials which can be used for several applications: [1][2][3][4] as sorbents for solid phase extraction (SPE), [5][6][7][8][9] as stationary phase for chromatography, [10][11][12][13][14][15] as sensors, [16][17][18] artificial enzymes, [19][20][21] as systems for controlled drug delivery and release, [22][23][24] etc. Molecular imprinting technology (MIT) is based on interactions between template molecule and functional monomer in the presence of porogen.…”

Section: Introductionmentioning

confidence: 99%

Highly selective water-compatible molecularly imprinted polymers for benzophenone-4

Pešić

Krstić

Verbić

2023

JSCS

View full text Add to dashboard Cite

Molecularly imprinting technology was applied for preparing selective sorbents for benzophenone-4 (BP4), an organic UV filter used in sunscreens and cosmetics. Several imprinted polymers were prepared by bulk polymerization, using BP4 as template. Combination of stability (mechanical and chemical), selectivity and robustness of imprinted polymers with BP4 properties, resulted in successful imprinting process (imprinting factors in range 1.05-2.60). Prepared polymers were characterized by infrared spectroscopy, elemental analysis, conductometric titrations and nitrogen physisorption at 77 K. Adsorption capacities and selectivity towards 7 other organic UV filters (benzophenone-3, benzophenone-8, homosalate, butyl methoxydiben-zoylmethane, ethyl hexyl salicylate, ethyl hexyl p-dimethylamino benzoate and ethyl hexyl p-methoxycinnamate) were determined, proving high adsorption capacity and high selectivity for BP4 binding. Highest adsorption capacity had 4-vinyl pyridine/ethylene glycol dimethacrylate co-polymer prepared in dimethyl sulfoxide (1.108 mmol g-1). Imprinted polymer with highest binding capacity was applied to solid phase extraction of BP4 from aqueous solutions with 98.5 % efficiency.

show abstract

“…Molecularly imprinted polymers (MIPs) with the ability to recognize molecular targets can be used as novel sorbents for solid phase extraction to extract SLX from biological fluids [ 7 ]. MIP is a polymeric porous material with complementary binding features concerning the molecule’s structure, shape, and spatial orientation [ 8 ]. As a smart synthetic material, MIP is extensively used in numerous areas including sensors, chromatography, drug delivery systems, and environmental separations/analysis [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Dynamic Study of Mechanism Underlying Nature of Molecular Recognition and the Role of Crosslinker in the Synthesis of Salmeterol-Targeting Molecularly Imprinted Polymer for Analysis of Salmeterol Xinafoate in Biological Fluid

et al. 2022

View full text Add to dashboard Cite

The rational preparation of molecularly imprinted polymers (MIPs) in order to have selective extraction of salmeterol xinafoate (SLX) from serum was studied. SLX is an acting β-adrenergic receptor agonist used in the treatment of asthma and has an athletic performance-enhancing effect. Molecular dynamics were used for the simulation of the SLX-imprinted pre-polymerization system, to determine the stability of the system. The computational simulation showed that SLX as a template, 4-hydroxyethyl methacrylate (HEMA) as a monomer, and trimethylolpropane trimethacrylate (TRIM) as a crosslinker in mol ratio of 1:6:20 had the strongest interaction in terms of the radial distribution functional. To validate the computational result, four polymers were synthesized using the precipitation polymerization method, and MIP with composition and ratio corresponding with the system with the strongest interaction as an MD simulation result showed the best performance, with a recovery of 96.59 ± 2.24% of SLX in spiked serum and 92.25 ± 1.12% when SLX was spiked with another analogue structure. Compared with the standard solid phase extraction sorbent C-18, which had a recovery of 79.11 ± 2.96%, the MIP showed better performance. The harmony between the simulation and experimental results illustrates that the molecular dynamic simulations had a significant role in the study and development of the MIPs for analysis of SLX in biological fluid.

show abstract

Design, synthesis and characterization of enzyme-analogue-built polymer catalysts as artificial hydrolases

Cited by 19 publications

References 65 publications

Molecular Imprinting on Nanozymes for Sensing Applications

Molecular Imprinting on Nanozymes for Sensing Applications

Highly selective water-compatible molecularly imprinted polymers for benzophenone-4

Molecular Dynamic Study of Mechanism Underlying Nature of Molecular Recognition and the Role of Crosslinker in the Synthesis of Salmeterol-Targeting Molecularly Imprinted Polymer for Analysis of Salmeterol Xinafoate in Biological Fluid

Contact Info

Product

Resources

About