Design Strategies for Water-Soluble Small Molecular Chromogenic and Fluorogenic Probes

Fluorescence Responses of the Protonation and Deprotonation Processes between Phenolate and Phenol within Rosamine

“…The lysosomal pH may modulate mitochondrial function and lifespan in eukaryotic cells. [28] The pH range of mammalian was slightly basic in active mitochondria (~8.0), [37] and the structures of probes 1a and 1b are similar to that of the commercial mitochondrial marker (MitoTracker® Red CMXRos), so the probe 1a and mito-marker were subjected to laser confocal cell experiments using HeLa cancer cells and Ges-1 normal cells, respectively. In the course of the experiment, 50 nmol/L commercial mitochondria marker was incubated with cells for 5 min to locate the intracellular mitochondria and then 300 nmol/L of probe 1a was added to continue culture.…”

“…At present, lots of pH fluorescent probes have been developed and used, the protonation and deprotonation of phenol group were used in cyanine, [24,25] BODIPY, [26,27] and other fluorophores. [28,29] The structure of rosamine is different from rhodamine with the absence of carboxyl group, [30] which can form the five-member ring in detection, [31] and rosamine is used for a kind of red emission fluorophore. [32] Its related probes, such as commercial mitochondrial markers (MitoTracker® Red CMXRos), RhSN-mito, rhodamine 123 [33,34] and so on, were known as mitochondria biomarkers, [35] and a red-emitting lysosome-specific probe based on rosamine was once reported with the protonation of dialkylamino group; [36] some other related compounds can be applied in selectively staining pluripotent stem cells.…”

The benzo[b]quinolizinium ion as a water-soluble platform for the fluorimetric detection of biologically relevant analytes

“…The detection of analytes with fluorescent probes, also referred to as chemosensors, 1 is a useful and versatile technique in chemistry and biology as well as in their neighboring disciplines, [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] because it offers the advantages of fluorescence spectroscopy, e.g. high sensitivity and straightforward detection procedure, combined with the high potential of chemical synthesis to prepare a fluorophore with the required substitution pattern.…”

“…2 The main principles of fluorescent sensing by organic chemosensors are well known and extensively described in recent reviews and monographs. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] In general, three different physical quantities may be determined from the emission of a molecule, namely emission energy, emission quantum yield and emission lifetime. 14,16 Therefore, essentially all fluorescent probes indicate a target analyte or a physical input (e.g.…”

A fast‐responsive fluorescent probe for sulfite and its bioimaging

“…However, these methods usually require tedious sample and reagent preparation or complicated instruments that are not suitable for routine analysis. As an alternative, the fluorescence method has received much attention due its the advantages of convenience, high sensitivity, fast response and ease of live-cell imaging (14)(15)(16). Fluorescent probes for SO 3 2-have been mainly based on the addition to an aldehyde moiety or a nucleophilic addition (17)(18)(19)(20)(21)(22)(23)(24)(25), and levulinate cleavage mediated by SO 3 2- (26)(27)(28)(29)(30)(31)(32)(33)(34)(35).…”