Design Platforms of Nanocapsules for Human Therapeutics or Vaccines

Kawano, Masaaki; Li, Xing; Lam, Kit S.; Handa, Hiroshi; Miyamura, Tatsuo; Barnett, Susan W.; Srivastava, Indresh K.; Cheng, R. Holland

doi:10.1002/9781118023648.ch5

Cited by 3 publications

(2 citation statements)

References 55 publications

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“…The baculovirus expression vector-based production of CP in commercially available insect High Five cells generates HEV-Cys-VLP capsid protein that is easily purified at high yield and low cost [25]. In contrast, many other nanodelivery systems are compromised due to variable expression levels and inefficiency in production.…”

Section: Discussionmentioning

confidence: 99%

Chemically Activatable Viral Capsid Functionalized for Cancer Targeting

Chen

Stark

et al. 2016

Nanomedicine (Lond.)

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Aim To design a theranostic capsule using the virus-like nanoparticle of the hepatitis E virus modified to display breast cancer cell targeting functional group (LXY30). Methods Five surface-exposed residues were mutated to cysteine to allow conjugation to maleimide-linked chemical groups via thiol-selective linkages. Engineered virus-like nanoparticles were then covalently conjugated to a breast cancer recognized ligand, LXY30 and an amine-coupled near-infrared fluorescence dye. Results LXY30-HEV VLP was checked for its binding and entry to a breast cancer cell line and for tumor targeting in vivo to breast cancer tissue in mice. The engineered virus-like nanoparticle not only targeted cancer cells, but also appeared immune silent to native hepatitis E virus antibodies due to epitope disruption at the antibody-binding site. Conclusion These results demonstrate the production of a theranostic capsule suitable for cancer diagnostics and therapeutics based on surface modification of a highly stable virus-like nanoparticle.

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Section: Discussionmentioning

confidence: 99%

Chemically Activatable Viral Capsid Functionalized for Cancer Targeting

Chen

Stark

et al. 2016

Nanomedicine (Lond.)

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“…In addition, HEVNP maintains its structure integrity of virus like particle in low-pH environment [3], an advantage for intratumoral penetration. The baculovirus expression vector-based production of HEV capsid protein, PORF2, in commercially-available insect High Five cells generates HEVNPs that are easily purified at high yield and low cost [4]. HEVNPs form a hollow, T=1 icosohedral capsid, composed of 60 identical units [5], rendering it highly stable both in storage and in harsh physiological conditions.…”

Section: Hepatitis E Virus Nanoparticles (Hevnps) As Nano-capsule Delivery Platformmentioning

confidence: 99%

Hepatitis E Virus Nanoparticle Encapsulating Nano-Theranostic Reagent as Modularized Capsule

Cheng¹

2017

ARGH

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A modularized theranostic capsule, Hepatitis E Virus Nanoparticle (HEVNP), derived from the HEV virus-like particle (VLP), is proposed as a multifunctional platform for cancer targeting, imaging and therapeutic applications. This can be achieved by utilizing the flexible domain of HEVNPs to conjugate different functional peptides and/or compounds to capsule surface, and simultaneously encapsulate drugs, DNA/RNA, or even inorganic beads into its interior. In prior research, breast cancer tumor targeting with a HEVNP capsule diagnostic was demonstrated by chemically conjugating a breast cancer cell-specific ligand and a near-infrared (NIR) fluorescence dye to its surface for diagnostic imaging.Here, we further explore applications of the modularized theranostic capsule by encapsulating ferrite nano-particles into the HEVNP interior for MRI detection and hyperthermia treatment.

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TRAPped Structures: Making Artificial Cages with a Ring Protein

Heddle

2017

ACS Symposium Series

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Design Platforms of Nanocapsules for Human Therapeutics or Vaccines

Cited by 3 publications

References 55 publications

Chemically Activatable Viral Capsid Functionalized for Cancer Targeting

Chemically Activatable Viral Capsid Functionalized for Cancer Targeting

Hepatitis E Virus Nanoparticle Encapsulating Nano-Theranostic Reagent as Modularized Capsule

TRAPped Structures: Making Artificial Cages with a Ring Protein

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