Design Parameters for Engineering Bone Regeneration

Guldberg, Robert E.; Duty, Angel O.

doi:10.1007/0-387-21547-6_12

Cited by 2 publications

(3 citation statements)

References 88 publications

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“…The delivery of human mesenchymal stem cells (hMSC) induced to differentiate toward osteoblasts on biodegradable scaffolds has resulted in promising results for bone formation in vitro and in vivo. 5 Porous silk scaffolds offer advantages in mechanical strength (average compressive strength ¼ 100 AE 10 kPa, elastic modulus ¼ 1300 AE 40 kPa), 6 biodegradability, and biocompatibility as a biomaterial platform for regenerating bone. [6][7][8] An important issue with any tissue-engineered bone implant is matching the tissue engineering strategy to patient's and implant's site-specific bone regeneration rates to ensure enhanced integration and bone remodeling.…”

mentioning

confidence: 99%

“…[6][7][8] An important issue with any tissue-engineered bone implant is matching the tissue engineering strategy to patient's and implant's site-specific bone regeneration rates to ensure enhanced integration and bone remodeling. 5,9 A number of studies have been conducted to tailor biomaterial matrix design to meet specific bone repair needs. In the case of silk biomaterials, significant control over bone morphology can be achieved by altering scaffold design features, for example, stiffness, pore size, interconnectivity between pores, degradability, and processing methods.…”

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confidence: 99%

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Hypoxia and Amino Acid Supplementation Synergistically Promote the Osteogenesis of Human Mesenchymal Stem Cells on Silk Protein Scaffolds

Sengupta

Park

Patel

et al. 2010

Tissue Engineering Part A

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Tailoring tissue engineering strategies to match patient-and tissue-specific bone regeneration needs offers to improve clinical outcomes. As a step toward this goal, osteogenic outcomes and metabolic parameters were assessed when varying inputs into the bone formation process. Silk protein scaffolds seeded with human mesenchymal stem cells in osteogenic differentiation media were used to study in vitro osteogenesis under varied conditions of amino acid (lysine and proline) concentration and oxygen level. The cells were assessed to probe how the microenvironment impacted metabolic pathways and thus osteogenesis. The most favorable osteogenesis outcomes were found in the presence of low (5%) oxygen combined with high lysine and proline concentrations during in vitro cultivation. This same set of culture conditions also showed the highest glucose consumption, lactate synthesis, and certain amino acid consumption rates. On the basis of these results and known pathways, a holistic metabolic model was derived which shows that lysine and proline supplements as well as low (5%) oxygen levels regulate collagen matrix synthesis and thereby rates of osteogenesis. This study establishes early steps toward a foundation for patient-and tissue-specific matches between metabolism, repair site, and tissue engineering approaches toward optimized bone regeneration.

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confidence: 99%

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confidence: 99%

Hypoxia and Amino Acid Supplementation Synergistically Promote the Osteogenesis of Human Mesenchymal Stem Cells on Silk Protein Scaffolds

Sengupta

Park

Patel

et al. 2010

Tissue Engineering Part A

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“…However, the strength of polyacrylate hydrogels is rather low, typically in the range 1-100 kPa [19][20][21]. This is not enough for high-end practical applications, such as artificial cartilage, where the material is required to withstand loads of 3-17 MPa [11,22]. Thus, the methods for preparation of reinforced polymer gels are intensively elaborated [23,24].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Printed Shape Memory Gels Based on a Structured Disperse System with Hydrophobic Cellulose Nanofibers

Prosvirnina,

Bugrov,

Bobrova

et al. 2023

Polymers

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Inks for 3D printing were prepared by dispersing bacterial cellulose nanofibers (CNF) functionalized with methacrylate groups in a polymerizable deep eutectic solvent (DES) based on choline chloride and acrylic acid with water as a cosolvent. After 3D printing and UV-curing, the double-network composite gel consisting of chemically and physically crosslinked structures composed from sub-networks of modified CNF and polymerized DES, respectively, was formed. The rheological properties of inks, as well as mechanical and shape memory properties of the 3D-printed gels, were investigated in dynamic and static modes. It was shown that the optimal amount of water allows improvement of the mechanical properties of the composite gel due to the formation of closer contacts between the modified CNF. The addition of 12 wt% water results in an increase in strength and ultimate elongation to 11.9 MPa and 300%, respectively, in comparison with 5.5 MPa and 100% for an anhydrous system. At the same time, the best shape memory properties were found for an anhydrous system: shape fixation and recovery coefficients were 80.0 and 95.8%, respectively.

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Design Parameters for Engineering Bone Regeneration

Cited by 2 publications

References 88 publications

Hypoxia and Amino Acid Supplementation Synergistically Promote the Osteogenesis of Human Mesenchymal Stem Cells on Silk Protein Scaffolds

Hypoxia and Amino Acid Supplementation Synergistically Promote the Osteogenesis of Human Mesenchymal Stem Cells on Silk Protein Scaffolds

Three-Dimensional Printed Shape Memory Gels Based on a Structured Disperse System with Hydrophobic Cellulose Nanofibers

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