Design of using chamber system based on electrical impedance spectroscopy (EIS) to measure epithelial tissue

Calvo, Paulo César; Campo, Oscar; Guerra, Cristian; Castaño, Santiago; Fonthal, Faruk

doi:10.1016/j.sbsr.2020.100357

Cited by 10 publications

(6 citation statements)

References 24 publications

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“…Electrochemical impedance spectroscopy (EIS) is an important technique for studying the interfacial properties of the electrode [ 30 ]. The electron transfer resistance (R ct ) at the surface of the electrode may be related to the diameter of a semicircle and can be used to quantitatively describe the interfacial properties of the electrode [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Electrochemical application of cobalt nanoparticles-polypyrrole composite modified electrode for the determination of phoxim

Tefera

Tessema

Admassie

et al. 2021

Analytica Chimica Acta: X

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In this study, cobalt nanoparticles (CoNPs) were synthesized and cobalt nanoparticles modified glassy carbon electrode (CoNPs/GCE) was prepared by drop coating the nanoparticles on glassy carbon electrode. After preparing polypyrrole modified glassy carbon electrode (PPy/GCE) using electropolymerization of pyrrole in LiClO 4 solution, cobalt nanoparticles-polypyrrole composite modified glassy carbon electrode (CoNPs/PPy/GCE) was fabricated by drop coating the CoNPs on the PPy/GCE. Different characterization techniques such as scanning electron microscopy, transmission electron microscopy, energy dispersive spectroscopy, FTIR spectroscopy, electrochemical impedance spectroscopy and cyclic voltammetry were used to study the morphological structure and electrochemical behavior of the sensors. The results demonstrated that PPy chains interacted with CoNPs through donor-acceptor bonds. Among all the electrodes, CoNPs/PPy/GCE exhibited highest electroactive surface area and lowest electron transfer resistance towards phoxim. Under the optimal conditions, the sensor showed linear relationship between the reduction peak current and the concentration of phoxim in the range of 0.025 μM–12 μM with the detection limit as 4.5 nM. Besides, the composite electrode demonstrated excellent reproducibility, good stability and selectivity towards the possible interfering substances. All of these properties made CoNPs/PPy/GCE a suitable electrochemical sensor for the electrochemical determination of phoxim in water samples using square wave voltammetry.

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Section: Resultsmentioning

confidence: 99%

Electrochemical application of cobalt nanoparticles-polypyrrole composite modified electrode for the determination of phoxim

Tefera

Tessema

Admassie

et al. 2021

Analytica Chimica Acta: X

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“…The HCS (Fig. 2b) is chosen because it can easily achieve high output impedance, signal to noise ratio (SNR), and is fully programmable through external control voltages 23,24 . Up to three parallel current stimulation signals are used for the three independent chambers in the system.…”

Section: Electronic Circuits For Teer Measurementmentioning

confidence: 99%

“…The magnitude and phase values are determined for each frequency to obtain the impedance spectrum of the tissue sample, commonly referred to as electrical impedance spectroscopy (EIS). Due to its versatility of revealing impedance information across a wide range of frequencies, EIS is a widely-used technique to discover the impedance characteristics of tissue/cell-culture samples in Ussing Chambers, Organ-on-a-chip devices, and well inserts 8,12,19,20,23,[26][27][28][29][30][31] .…”

Section: Teer Calculationmentioning

confidence: 99%

A Microphysiological System for Studying Barrier Health of Live Tissues in Real Time

Chen,

Way,

Templeton

et al. 2024

Preprint

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Epithelial cells create barriers that protect many different components in the body from their external environment. The gut in particular carries bacteria and other infectious agents. A healthy gut epithelial barrier prevents unwanted substances from accessing the underlying lamina propria while maintaining the ability to digest and absorb nutrients. Increased gut barrier permeability, better known as leaky gut, has been linked to several chronic inflammatory diseases. Yet understanding the cause of leaky gut and developing effective interventions are still elusive due to the lack of tools to maintain tissue’s physiological environment while elucidating cellular functions under various stimuli ex vivo. This paper presents a microphysiological system capable of recording real-time barrier permeability of mouse gut tissues in a realistic physiological environment over extended durations. Key components of the microphysiological system include a microfluidic chamber designed to hold the live tissue explant and create a sufficient microphysiological environment to maintain tissue viability; proper media composition that preserves a microbiome and creates necessary oxygen gradients across the barrier; integrated sensor electrodes and supporting electronics for acquiring and calculating transepithelial electrical resistance (TEER); and a scalable system architecture to allow multiple chambers running in parallel for increased throughput. The experimental results demonstrate that the system can maintain tissue viability for up to 72 hours. The results also show that the custom-built and integrated TEER sensors are sufficiently sensitive to distinguish differing levels of barrier permeability when treated with collagenase and low pH media compared to control. Permeability variations in tissue explants from different positions in the intestinal tract were also investigated using TEER revealing their disparities in permeability. Finally, the results also quantitatively determine the effect of the muscle layer on total epithelial resistance.

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“…It involves measuring the electrical impedance of a system over a range of frequencies and is particularly useful for characterizing the electrical properties of biological tissues [1,2], cells [3,4], and other medical materials as biosensors [5,6]. Concerning biological tissue analysis, EIS offers a non-invasive and label-free approach to assess tissue composition, hydration, and physiological changes [7]. By measuring the impedance spectrum, EIS can provide information about tissue architecture, cellular organization, and even detect abnormal conditions such as cancer.…”

Section: Introductionmentioning

confidence: 99%

The Use of Electrical Impedance Spectroscopy for Medical Application: A Mini Review

2023

PSBJ

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Electrical impedance spectroscopy (EIS) has emerged as a powerful technique in biophysics, enabling the analysis of biological tissues, cell behavior, and the development of biosensors. By measuring the impedance response of biological systems across a range of frequencies, EIS provides valuable insights into the electrical properties and structural characteristics of tissues and cells. This paper provides an overview of fundamental principles of EIS and the application of impedance spectroscopy in biophysic, highlighting its potential in understanding tissue properties, monitoring cell behavior, and designing biosensors for various biomedical applications.

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Design of using chamber system based on electrical impedance spectroscopy (EIS) to measure epithelial tissue

Cited by 10 publications

References 24 publications

Electrochemical application of cobalt nanoparticles-polypyrrole composite modified electrode for the determination of phoxim

Electrochemical application of cobalt nanoparticles-polypyrrole composite modified electrode for the determination of phoxim

A Microphysiological System for Studying Barrier Health of Live Tissues in Real Time

The Use of Electrical Impedance Spectroscopy for Medical Application: A Mini Review

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