Design of the mammalian respiratory system. IV. Morphometric estimation of pulmonary diffusing capacity; Critical evaluation of a new sampling method

Weibel, Ewald R.; Gehr, Peter; Cruz‐Orive, Luis M.; Müller, Alfred; Mwangi, D K; Haussener, Virginia

doi:10.1016/0034-5687(81)90076-1

Cited by 36 publications

(15 citation statements)

References 23 publications

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“…The surface fraction of SP-labelled AEII was determined according to the point and intersection counting method [30]. Therefore, we counted the number of intersections with labelled AEII and with the alveolar surface using an intersection test system.…”

Section: Methodsmentioning

confidence: 99%

Increased Surfactant Protein A and D Expression in Acute Ovalbumin-Induced Allergic Airway Inflammation in Brown Norway Rats

Schmiedl

Lührmann

Pabst

et al. 2008

Int Arch Allergy Immunol

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Background: The surfactant proteins SP-A and SP-D, components of the innate immune system, are involved in host defence. Objective: We tested the hypothesis that ovalbumin (OVA) challenge leads to an upregulation of both proteins in alveolar epithelial type II cells (AEII) and Clara cells and to an enhanced uptake by macrophages. Methods: After sensitization with OVA and heat-killed Bordetella pertussis challenge followed intratracheally with 0.5% OVA on day 13. One day after challenge lung tissue and bronchoalveolar lavage fluid (BALF) of sensitized NaCl- and OVA-challenged Brown Norway rats were compared with home cage controls using qRt-PCR, Western blot and immunohistochemistry. Results: After OVA challenge (1) eosinophils increased significantly in the BALF, (2) the total amount of SP-A and SP-D was significantly increased in lung tissue, (3) the amount of SP-A was significantly and the amount of SP-D was remarkably elevated in BALF, and (4) the levels of SP-A and SP-D mRNA in lung tissue were significantly elevated. Using quantitative immunohistochemistry, we found (5) significantly higher surface fractions of SP-A- and SP-D-labelled AEII, (6) no differences in the surface fractions of SP-A- and SP-D-labelled bronchial Clara cells, and (7) a significantly increased cell density of unlabelled and SP-A-labelled macrophages. Conclusions: Thus, combining molecular biological and histological methods we suggest that after OVA challenge (1) AEII but not Clara cells show a significantly higher expression of SP-A and SP-D leading also to higher amounts of both SPs in BALF and (2) macrophages gather predominantly SP-A.

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Section: Methodsmentioning

confidence: 99%

Increased Surfactant Protein A and D Expression in Acute Ovalbumin-Induced Allergic Airway Inflammation in Brown Norway Rats

Schmiedl

Lührmann

Pabst

et al. 2008

Int Arch Allergy Immunol

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“…The fine structure of lung parenchyma (see for instance Weibel et al, 1981) may be studied in a model based setting - Miles (1978b) called this the extended case, revisited by CruzOrive (2009). To keep variation within useful bounds, however, the size of T should be large enough to encompass the 'typical features' of the target structure (Lantuejoul, 1991(Lantuejoul, , 2002.…”

Section: Model Based Stereologymentioning

confidence: 99%

Stereology: A Historical Survey

Cruz‐Orive

2017

Image Anal Stereol

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Stereology is the science of geometric sampling, with applications to the statistical analysis of microstructures in biology and materials science. Subsidiary disciplines are image analysis, quantitative microscopy, and radiology. This survey is organized chronologically within a series of topics which cover most aspects of stereology. Each topic is described informally to make it accessible to scientists of different disciplines.

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“…where the alveolar surface is expressed as the product of lung volume (V p ) and the surface density per unit of lung volume (dSa), κ is Krogh's constant and VO 2 is the oxygen consumption (Weibel et al, 1981). Thus, high oxygen consumption may be achieved through increases in alveolar surface density or lung volume, or by reducing the thickness of the alveolar-capillary barrier.…”

Section: Alveolar Diffusionmentioning

confidence: 99%

Biomechanical, Respiratory and Cardiovascular Adaptations of Bats and the Case of the Small Community of Bats in Chile

Canals¹,

Iriarte-Díaz²,

Grossi³

2011

Biomechanics in Applications

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Design of the mammalian respiratory system. IV. Morphometric estimation of pulmonary diffusing capacity; Critical evaluation of a new sampling method

Cited by 36 publications

References 23 publications

Increased Surfactant Protein A and D Expression in Acute Ovalbumin-Induced Allergic Airway Inflammation in Brown Norway Rats

Increased Surfactant Protein A and D Expression in Acute Ovalbumin-Induced Allergic Airway Inflammation in Brown Norway Rats

Stereology: A Historical Survey

Biomechanical, Respiratory and Cardiovascular Adaptations of Bats and the Case of the Small Community of Bats in Chile

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