Design of surface ligands for blood compatible gold nanoparticles: Effect of charge and binding energy

Beurton, Jordan; Lavalle, Philippe; Pallotta, Arnaud; Chaigneau, Thomas; Clarot, Igor; Boudier, Ariane

doi:10.1016/j.ijpharm.2020.119244

Cited by 24 publications

(13 citation statements)

References 36 publications

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“…According to Ross and Subramanian [ 22 ], the positive values of the thermodynamic constants (ΔS and ΔH) for Fe 3 O 4 , Fe 3 O 4 @Ag, and Fe 3 O 4 @Ag/TCS NPs evidenced hydrophobic interactions with albumin [ 40 ]. The NP–protein association is frequently driven by weak interactions, and its mechanism enables the surface stabilization of each particle [ 41 ]. Hydrophobic interaction is the result of a partial withdrawal of the nonpolar group of water molecules and the solvation layer of the three NPs and/or albumin.…”

Section: Resultsmentioning

confidence: 99%

The Impact of Multiple Functional Layers in the Structure of Magnetic Nanoparticles and Their Influence on Albumin Interaction

Pieretti

Beurton

Munévar

et al. 2021

IJMS

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In nanomedicine, hybrid nanomaterials stand out for providing new insights in both the diagnosis and treatment of several diseases. Once administered, engineered nanoparticles (NPs) interact with biological molecules, and the nature of this interaction might directly interfere with the biological fate and action of the NPs. In this work, we synthesized a hybrid magnetic nanostructure, with antibacterial and antitumoral potential applications, composed of a magnetite core covered by silver NPs, and coated with a modified chitosan polymer. As magnetite NPs readily oxidize to maghemite, we investigated the structural properties of the NPs after addition of the two successive layers using Mössbauer spectroscopy. Then, the structural characteristics of the NPs were correlated to their interaction with albumin, the major blood protein, to evidence the consequences of its binding on NP properties and protein retention. Thermodynamic parameters of the NPs–albumin interaction were determined. We observed that the more stable NPs (coated with modified chitosan) present a lower affinity for albumin in comparison to pure magnetite and magnetite/silver hybrid NPs. Surface properties were key players at the NP–biological interface. To the best of our knowledge, this is the first study that demonstrates a correlation between the structural properties of complex hybrid NPs and their interaction with albumin.

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Section: Resultsmentioning

confidence: 99%

The Impact of Multiple Functional Layers in the Structure of Magnetic Nanoparticles and Their Influence on Albumin Interaction

Pieretti

Beurton

Munévar

et al. 2021

IJMS

Self Cite

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“…[2] Hemocompatibility can also be affected by the presence of BC, leading to a change in the interaction between nanostructures and blood components. [39,40] Blood interaction with BC-protein complexes can be made more complex by the interaction with anticoagulants. For example, it has been shown that the BC formed on the surface of poly(lactic acid) (PLA), polycaprolactone (PCL), silica, and a polystyrene vascular targeted carrier when exposed to both serum and whole blood is significantly affected by the presence of heparin, demonstrating an increase in their vascular targeting and adhesion efficiency.…”

Section: The Impact Of the Biomolecular Coronamentioning

confidence: 99%

“…In some studies, fluorescence quenching of both the endogenous fluorescence of proteins and fluorescently labeled proteins has been used to investigate the interaction between proteins and nanostructures. [24,40,133] One interesting example of fluorescently labeled protein use in BC studies is the work by Pinals et al, in which the exchange rate of two fluorescently labeled blood abundant proteins (namely, fibrinogen and albumin) on the surface of single-walled carbon nanotubes (SWCNTs) were investigated, functionalized with single-strand DNA (SSDNA) acting as a biosensor for dopamine. [133] The binding of both proteins and SSDNA fluorescently labeled on the surface of the SWCNT was monitored through the fluorescence quenching effect caused by the proximity of the fluorophore to the SWCNT surface.…”

Section: Indirect Techniques Based On Scattering Spectroscopy Colorim...mentioning

confidence: 99%

Biomolecular Corona Associated with Nanostructures: The Potentially Disruptive Role of Raman Microscopy

Battaglini

Gümüş

Ciofani

2021

Adv Materials Technologies

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investigating the use of nanomaterials within living organisms. When a nanoparticle (NP) encounters biological molecules such as proteins, lipids, nucleic acids, or polysaccharides, it tends to adsorb these molecules on its surface due to electrostatic, hydrophobic, or other forms of interactions between the molecules and the surface of the NP. [1][2][3][4] Depending on their abundance and affinity, proteins and other molecules may form a hard corona that consists of tightly bounded proteins on the surface, or a soft corona, which indicates a second layer of proteins that are loosely attached to the proteins of the hard corona. [4] The composition of the soft corona changes over time according to the environmental conditions, and so the biological identity of nanostructures is usually, but not always, determined by the hard corona. This can be both advantageous and disadvantageous to the application of NPs. Some studies have focused on preventing biomolecular corona (BC) formation, as this may change the size and surface properties, hinder modifications on the surface, and cause the rapid detection and clearance of NPs by the immune system. [5,6] However, BCs can be exploited or even altered, in order to be made beneficial for targeting purposes as they can enable specific cells to more easily recognize NPs and may lead to a reduction in their toxicity. [7,8] A wide range of techniques have been used in BC studies, such as dynamic light scattering (DLS), [9] sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), mass spectrometry (MS)-based approaches, [10][11][12][13][14][15][16][17][18][19][20][21] UV/vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), [22] atomic force microscopy (AFM), [23] scanning electron microscopy (SEM), [24][25][26][27] transmission electronic microscopy (TEM), [23] and circular dichroism spectroscopy (CD). [28] Various information (size, surface properties, morphology, structure, etc.) about BCs can be obtained through these methods. Many techniques have been applied in BC studies, which can be divided into microscopybased approaches that use direct imaging of the sample, and indirect techniques. Microscopy techniques can provide information about corona composition and shape with a spatial resolution up to the dimension scale of single protein molecules, but they are limited by the requirement for staining or extensive sample preparation. Indirect techniques can provide extensive information about BC-nanostructure interactions and properties, but typically have a limited resolution and so cannot analyze the BC at the level of single nanostructures. Other emerging

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“…toxic cationic surfactant [84][85][86] and the conjugate systems of AuNWs-CTAB are proven to still contribute to toxicity to culture cells especially when the elevated concentrations of CTAB are present (50 g/ml commercial AuNWs-CTAB). With increasing the amount of AuNWs-CTAB solution, the cytotoxicity of the sample is increased caused by the nonspeci c binding tendency of CTAB to negatively charged cell surfaces by electrostatic interactions [87,88]. Once the interaction with the cell occurs, it forms blebs and holes on the cell and leading to cell death evident by the increase in the diameter of the ring zone.…”

Section: Cytotoxicity Evaluation Of As-synthesised and Commercial Aunwsmentioning

confidence: 99%

Synthesis, Characterisation and Cytotoxicity of Gold Nanowires for Ultra-Sensitive Biosensor Development

Lah

Gray

Trigueros

2020

Preprint

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With the long-term goal of developing an ultra-sensitive microcantilever-based nano biosensor for versatile biomarker detection, new controlled bioreceptor-analytes systems are being explored to overcome the disadvantages of conventional ones. Gold nanowires (AuNWs) have been used as a probe to overcome the tolerance problem that occurs in response to changes in environmental conditions. However, the cytotoxicity of AuNWs is still unclear. Here, we examined the cytotoxicity of AuNWs systems using both commercial and as-synthesised AuNWs. In vitro experiments show that commercial AuNWs with an average quoted length of 6 µm are highly toxic against Gram-negative Escherichia coli (E. coli) at 50 µg/ml. However, this toxicity is due to the presence of CTAB surfactant not by the nanostructure. Conversely, the as-synthesised AuNWs with an average length of 9.5 µm show non-cytotoxicity even at the maximum viable concentration (330µg/ml). These findings may lead to the development of potentially life-saving cytotoxicity -free nano biosensors for an early diagnostic of potential diseases.

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Design of surface ligands for blood compatible gold nanoparticles: Effect of charge and binding energy

Cited by 24 publications

References 36 publications

The Impact of Multiple Functional Layers in the Structure of Magnetic Nanoparticles and Their Influence on Albumin Interaction

The Impact of Multiple Functional Layers in the Structure of Magnetic Nanoparticles and Their Influence on Albumin Interaction

Biomolecular Corona Associated with Nanostructures: The Potentially Disruptive Role of Raman Microscopy

Synthesis, Characterisation and Cytotoxicity of Gold Nanowires for Ultra-Sensitive Biosensor Development

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