Design of SERS nanotags for multiplexed lateral flow immunoassays

Sánchez-Purrà, Maria; Roig-Solvas, Biel; Versiani, Alice F.; Rodriguez-Quijada, Cristina; Puig, Helena de; Bosch, Irene; Gehrke, Lee; Hamad‐Schifferli, Kimberly

doi:10.1039/c7me00052a

Cited by 34 publications

(31 citation statements)

References 41 publications

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“…Among many different approaches, microfluidic devices, lateral-flow assays, and lab-on-a-chip assays are the most common diagnostic assay platforms that have been developed and tested for infectious diseases [94,[115][116][117][118][119][120]. SERS-based assays have been integrated into these devices to be used for both qualitative and quantitative biomarker detection [7,8,70,73,91,94,[121][122][123][124]. Integration of SERS detection schemes into lateral flow assays are commonly seen in the recent work related to infectious disease diagnosis [91,122].…”

Section: Sers-based Diagnostic Devices and Assay Platformsmentioning

confidence: 99%

“…In comparison to the enzyme-linked immunosorbent assay (ELISA)-based method, the SERS-based LFA provides approximately three orders of magnitude higher detection sensitivity. Hamad-Schifferli's group also developed LFAs using SERS nanotags for dengue and Zika detection [70,125]. The importance of their study is that they systematically examined the importance of gold nanostar morphological features and Raman reporter selection in order optimize the SERS enhancement and multiplexed detection in LFAs, respectively.…”

Section: Sers-based Diagnostic Devices and Assay Platformsmentioning

confidence: 99%

“…The importance of their study is that they systematically examined the importance of gold nanostar morphological features and Raman reporter selection in order optimize the SERS enhancement and multiplexed detection in LFAs, respectively. They investigated the effect of the morphological features of gold nanoparticles (spherical vs. star-shaped and the varying tip curvature of gold nanostars) as SERS substrates on the sensitivity of SERS-based LFA [70]. The SERS enhancement of the Raman reporter molecules 1,2-bis(4-pyridyl)ethylene and 4-mercaptobenzoic acid were optimized and compared using different types of gold nanoparticles for multiplexed detection of zika and dengue non-structural protein 1 (NS1).…”

Section: Sers-based Diagnostic Devices and Assay Platformsmentioning

confidence: 99%

“…In general, SERS-based sensing approaches have become more attractive and advantageous over conventional methods such as fluorescence due to ultra-sensitivity, even up to single-molecule detection, rich spectroscopic information provided on the molecular structure of interest that enable direct biomarker detection, and higher multiplexing capability [8,34,[60][61][62][63][64][65][66][67][68][69][70]. Among these advantages, the multiplexed SERS detection, in particular, is very attractive in clinical settings as it allows for the detection of a panel of biomarkers ensuring higher confidence in the diagnosis and poses as a better alternative to the commonly used fluorescence-based multiplexed biosensing.…”

Section: Introductionmentioning

confidence: 99%

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Recent Advancement in the Surface-Enhanced Raman Spectroscopy-Based Biosensors for Infectious Disease Diagnosis

2019

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Diagnosis is the key component in disease elimination to improve global health. However, there is a tremendous need for diagnostic innovation for neglected tropical diseases that largely consist of mosquito-borne infections and bacterial infections. Early diagnosis of these infectious diseases is critical but challenging because the biomarkers are present at low concentrations, demanding bioanalytical techniques that can deliver high sensitivity with ensured specificity. Owing to the plasmonic nanomaterials-enabled high detection sensitivities, even up to single molecules, surface-enhanced Raman spectroscopy (SERS) has gained attention as an optical analytical tool for early disease biomarker detection. In this mini-review, we highlight the SERS-based assay development tailored to detect key types of biomarkers for mosquito-borne and bacterial infections. We discuss in detail the variations of SERS-based techniques that have developed to afford qualitative and quantitative disease biomarker detection in a more accurate, affordable, and field-transferable manner. Current and emerging challenges in the advancement of SERS-based technologies from the proof-of-concept phase to the point-of-care phase are also briefly discussed.

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Section: Sers-based Diagnostic Devices and Assay Platformsmentioning

confidence: 99%

Section: Sers-based Diagnostic Devices and Assay Platformsmentioning

confidence: 99%

Section: Sers-based Diagnostic Devices and Assay Platformsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recent Advancement in the Surface-Enhanced Raman Spectroscopy-Based Biosensors for Infectious Disease Diagnosis

2019

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“…SERS signals are based on the increase of optical absorption for reporter molecules by orders of magnitude after their immobilization on the surface of nanoparticles. The possibility of such highly sensitive analyses is demonstrated in the works of Sanchez-Purra et al [110], Fu et al [111], and Marks et al [112]. Clarke et al [113] described the combination of SERS registration with rapid vertical flow technology as an additional means of increasing sensitivity.…”

Section: Proper Response For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

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Metal Nanoparticles‐Enhanced Biosensors: Synthesis, Design and Applications in Fluorescence Enhancement and Surface‐enhanced Raman Scattering

Yaraki

Tan

2020

Chemistry An Asian Journal

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Metal nanoparticles (NP) that exhibit localized surface plasmon resonance play an important role in metalenhanced fluorescence (MEF) and surface-enhanced Raman scattering (SERS). Among the optical biosensors, MEF and SERS stand out to be the most sensitive techniques to detect a wide range of analytes from ions, biomolecules to macromolecules and microorganisms. Particularly, anisotropic metal NPs with strongly enhanced electric field at their sharp corners/edges under a wide range of excitation wavelengths are highly suitable for developing the ultrasensitive plasmonenhanced biosensors. In this review, we first highlight the reliable methods for the synthesis of anisotropic gold NPs and silver NPs in high yield, as well as their alloys and composites with good control of size and shape. It is followed by the discussion of different sensing mechanisms and recent advances in the MEF and SERS biosensor designs. This includes the review of surface functionalization, bioconjugation and (directed/self) assembly methods as well as the selection/screening of specific biorecognition elements such as aptamers or antibodies for the highly selective biodetection. The right combinations of metal nanoparticles, biorecognition element and assay design will lead to the successful development of MEF and SERS biosensors targeting different analytes both in-vitro and in-vivo. Finally, the prospects and challenges of metal-enhanced biosensors for future nanomedicine in achieving ultrasensitive and fast medical diagnostics, high-throughput drug discovery as well as effective and reliable theranostic treatment are discussed.

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Design of SERS nanotags for multiplexed lateral flow immunoassays

Cited by 34 publications

References 41 publications

Recent Advancement in the Surface-Enhanced Raman Spectroscopy-Based Biosensors for Infectious Disease Diagnosis

Recent Advancement in the Surface-Enhanced Raman Spectroscopy-Based Biosensors for Infectious Disease Diagnosis

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Metal Nanoparticles‐Enhanced Biosensors: Synthesis, Design and Applications in Fluorescence Enhancement and Surface‐enhanced Raman Scattering

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