Design of prevascularized three-dimensional cell-dense tissues using a cell sheet stacking manipulation technology

Sasagawa, Tadashi; Shimizu, Tatsuya; Sekiya, Sachiko; Haraguchi, Yuji; Yamato, Masayuki; Sawa, Yoshiki; Okano, Teruo

doi:10.1016/j.biomaterials.2009.11.036

Cited by 279 publications

(195 citation statements)

References 27 publications

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“…In the herein presented work, it was shown that HUVECs, seeded over rBMSCs 4 days before the end of the osteogenic differentiation period, self-organized either in small round colonies or in elongated aggregates resembling a network. As these cocultured CSs were not cultured in vitro after stacking with a second osteogenic CSs, which could provide a 3D matrix for HUVECs reorganization, the formation of capillary-like structures, as seen in other works, 23,24,35 was not expected.…”

Section: Discussionmentioning

confidence: 90%

“…Similar efforts have been intense in the CS engineering field. 23,24,35 Sasagawa et al 24 seeded HUVECs between myoblast CSs, building five-layer constructs, to obtain in vitro a capillary-like network that anastomosed when implanted in nude rats, allowing the survival of the construct. Another work 23 showed that the positioning of endothelial cells within two fibroblast CS constructs was determinant for tubular formation and influences its lumen area in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…The prevascularization of CS-based 3D constructs has nevertheless been the focus of recent works. [23][24][25] The combination of several myoblasts sheets, recovered from PIPPAam dishes, and human umbilical vein endothelial cells (HUVECs) using a CS stacking methodology resulted in the generation of a prevascularized cell-dense tissue. 24 This work is a proof-of-concept on how CS engineering has the potential to produce 3D prevascularized tissues.…”

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confidence: 99%

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Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets

Pirraco

Iwata

Yoshida

et al. 2014

Laboratory Investigation

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Addressing the problem of vascularization is of vital importance when engineering three-dimensional (3D) tissues. Endothelial cells are increasingly used in tissue-engineered constructs to obtain prevascularization and to enhance in vivo neovascularization. Rat bone marrow stromal cells were cultured in thermoresponsive dishes under osteogenic conditions with human umbilical vein endothelial cells (HUVECs) to obtain homotypic or heterotypic cell sheets (CSs). Cells were retrieved as sheets from the dishes after incubation at 20 1C. Monoculture osteogenic CSs were stacked on top of homotypic or heterotypic CSs, and subcutaneously implanted in the dorsal flap of nude mice for 7 days. The implants showed mineralized tissue formation under both conditions. Transplanted osteogenic cells were found at the new tissue site, demonstrating CS bone-inductive effect. Perfused vessels, positive for human CD31, confirmed the contribution of HUVECs for the neovascularization of coculture CS constructs. Furthermore, calcium quantification and expression of osteocalcin and osterix genes were higher for the CS constructs, with HUVECs demonstrating the more robust osteogenic potential of these constructs. This work demonstrates the potential of using endothelial cells, combined with osteogenic CSs, to increase the in vivo vascularization of CS-based 3D constructs for bone tissue engineering purposes.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets

Pirraco

Iwata

Yoshida

et al. 2014

Laboratory Investigation

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“…The formation of capillary networks of HUVECs in the scaffolds or tissues by 3D-culture has been previously reported. 10,51,52 The development of 3D-vascularized thick tissues possessing high density bloodcapillary networks is still an outstanding issue for not only tissue engineering, but also for angiogenesis models for Open squares indicate the layer number using the 0.4¯m-pore insert with a medium change after 12 h of incubation. b) HE images of the obtained tissues using the 0.4¯m-pore insert.…”

Section: Rapid Construction Of 3d-tissues With Endothelial Tube Networkmentioning

confidence: 99%

“…A bottom-up approach using multiple cell types as pieces of tissue is expected to solve these problems. Currently, various technologies such as cell sheets 10 or magnetic liposomes 11 have been reported in constructing a multilayered cell sheet. These methods are intriguing examples of a bottom-up approach, but have limitations due to the complicated manipulation of the fragile cell sheet or the remains of magnetite particles in the cells.…”

Section: Introductionmentioning

confidence: 99%

Development of Three-Dimensional Tissue Models Based on Hierarchical Cell Manipulation Using Nanofilms

Matsusaki

2012

Bulletin of the Chemical Society of Japan

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The creation of artificial three-dimensional (3D) tissues possessing structure and function similar to natural tissue is a key challenge for implantable tissues in tissue engineering, and for model tissues in pharmaceutical assays. This account is a summary of our current research toward this challenge. We have developed a simple and unique bottom-up approach, hierarchical cell manipulation technique, using nanometer-sized layer-by-layer films consisting of fibronectin and gelatin (FNG) as a nano-extracellular matrix (nano-ECM). The FNG nanofilms were prepared directly on the cell surface, and we discovered that at least 6 nm thick FNG films acted as a stable adhesive surface for adhesion of the second cell layer. Various 3D-layered constructs consisting of single or multiple types of cells were successfully fabricated, and the higher cellular activities induced from the 3D-structures as compared to monolayer structure were observed. Furthermore, the multilayered constructs like a blood vessel wall structure indicated almost the same drug response as in vivo natural blood vessels, suggesting the possibility to use as an in vitro blood vessel model to analyze drug response. Recently, we also developed a rapid bottom-up approach by a single cell coating using FNG nanofilms, because the fabrication of twolayers (2L) is limited through the above technique due to the time required for stable cell adhesion. This rapid approach easily provided approximately eight-layered (8L) 3D-tissues after only one day of incubation. The layer number, cell type, and location were all successfully controlled by altering the seeding cell number and order. Moreover, fully and homogeneously vascularized tissues of 1 cm width and 50¯m height were obtained by a sandwich culture of the endothelial cells. These hierarchical cell manipulations will be promising to achieve one of the dreams of biomedical field, in vitro creation of artificial 3D-tissue models.

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Responsive Polymer Coatings for Smart Applications in Chromatography, Drug Delivery Systems, and Cell Sheet Engineering

Pirraco

Yamato

Akiyama

et al. 2012

Intelligent Surfaces in Biotechnology

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Design of prevascularized three-dimensional cell-dense tissues using a cell sheet stacking manipulation technology

Cited by 279 publications

References 27 publications

Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets

Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets

Development of Three-Dimensional Tissue Models Based on Hierarchical Cell Manipulation Using Nanofilms

Responsive Polymer Coatings for Smart Applications in Chromatography, Drug Delivery Systems, and Cell Sheet Engineering

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