Design of Monovalent and Chimeric Tetravalent Dengue Vaccine Using an Immunoinformatics Approach

Abstract

An immunoinformatics technique was used to predict a monovalent amide immunogen candidate capable of producing therapeutic antibodies as well as a potent immunogen candidate capable of acting as a universal vaccination against all dengue fever virus serotypes. The capsid protein is an attractive goal for anti-DENV due to its position in the dengue existence cycle. The widely accessible immunological data, advances in antigenic peptide prediction using reverse vaccinology, and the introduction of molecular docking in immunoinformatics have directed vaccine manufacturing. The C-proteins of DENV-1-4 serotypes were known as antigens to assist with logical design. Binding epitopes for TC cells, TH cells, and B cells is predicted from structural dengue virus capsid proteins. Each T cell epitope of C-protein integrated with a B cell as a templet was used as a vaccine and produce antibodies in contrast to serotype of the dengue virus. A chimeric tetravalent vaccine was created by combining four vaccines, each representing four dengue serotypes, to serve as a standard vaccine candidate for all four Sero groups. The LKRARNRVS, RGFRKEIGR, KNGAIKVLR, and KAINVLRGF from dengue 1, dengue 2, dengue 3, and dengue 4 epitopes may be essential immunotherapeutic representatives for controlling outbreaks.

Significance of Conserved Regions in Coronavirus Spike Protein for Developing a Novel Vaccine against SARS-CoV-2 Infection

“…For example, due to the multiple strains of influenza over the years, the means of developing a universal vaccine has been the use of the conserved epitopes on the hemagglutinin (HA stalk) or the matrix ectodomain (M2e) [ 98 , 99 , 100 , 101 , 102 , 103 , 104 ]. The strategy of using conserved epitopes has also been used in the development of preventative vaccines for HIV, dengue virus, Lassa fever virus (LASV), hepatitis virus and Kaposi’s sarcoma-associated herpesvirus (KSHV) [ 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 ]. Therefore, identifying the conserved regions in the SP of SARS-CoV variants will be of great importance in developing a universal vaccine against CoVs.…”

Immunoinformatics-Aided Design and In Vivo Validation of a Peptide-Based Multiepitope Vaccine Targeting Canine Circovirus

“…With the recent progress in the field of bioinformatics, different strategies have been employed to design knowledge-based vaccines using the immunoinformatics approach. − In fact, multiepitope vaccines (MEVs) have been designed for Epstein–Barr and Chlamydia trachomatis viruses using immunoinformatics, which are found to elicit effective humoral and cellular responses both in vitro and in vivo (18–20). Using a similar approach, we recently identified five epitopes, i.e., YQH­LPP­FRF, YIR­AKW­INW, ALY­RRL­TLI, HLQ­GFV­NLK, and GTM­NFV­ARR, from capsid and replicase proteins, which could be used to design a vaccine candidate for CanineCV .…”

Immunoinformatics design of a structural proteins driven multi-epitope candidate vaccine against different SARS-CoV-2 variants based on fynomer

“…From the immunoinformatic approaches used in this study for SARS-CoV-2 in the design of multi-epitope vaccines against other pathogens such as MERS-CoV 47 , Nipah virus 72 , Zika 73 , dengue 74 , Hendra virus 75 , Malaria 76 , Acinetobacter baumannii 77 , Leishmania infantum 78 , Pseudomonas aeruginosa 79 , Klebsiella pneumoniae 80 , Kaposi sarcoma 81 and several other examples have also been used. A similar strategy has also been used to make a vaccine against cancer antigens.…”