Design of Immunoradiometric Assay for Gonadotropins Using a Common Detection Antibody: An Experience

Abstract

We share our experience in the development of immunoradiometric assay (IRMA) for gonadotropins, FSH and LH using common tracer concept. Commercially procured, monoclonal antibody against 'common epitope' of FSH and LH was radiolabeled and epitope specific monoclonal antibody against β-subunit of FSH and LH was chemically immobilized and used as a capture system. In an inclusive assay procedure developed, 100 µl of respective standard and specific capture antibody was incubated with 100 µl common tracer, for 3 h at RT with shaking. Results of validation tests confirmed the clinical utility of the developed procedure with adequate sensitivity of 1.0 mIU/ml, acceptable precision (imprecision CV ≤ 10%), minimal cross-reaction.