Design of Functional Nanoparticles for Intractable Disease Therapy

Koide, Hiroyuki

doi:10.1248/bpb.b20-00699

Cited by 2 publications

(3 citation statements)

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“…Synthesis of NPs with High Affinity for the Target Peptide The functionalization of lightly cross-linked NIPAmbased NPs with acrylic acid (AAc) (negatively charged monomer) and N-tert-butylacrylamide (TBAm hydrophobic monomer) enables them to bind to and neutralize honey bee venom (i.e., melittin) both in vitro and in vivo. 16,18) Therefore, we used NIPAm-based NPs and melittin as a model of abiotic protein affinity reagent and toxic peptide, respectively, for the evaluation of NP function in vitro. NPs were prepared by free-radical copolymerization using NIPAm (18 mol%), Bis (2 mol%, cross-linker), TBAm (40 mol%), and AAc (40 mol%, Fig.…”

Section: Resultsmentioning

confidence: 99%

“…We and other groups purified synthesized synthetic polymers by dialysis against ultrapure water, 23,24) and the polymers showed high affinity for the target both in vitro and in vivo. 16,18) Therefore, NPs purified by dialysis against ultrapure water were used as control NPs (NP1). Synthesized NPs were added to the dialysis membrane and dialyzed against 100 volumes of ultrapure water.…”

Section: Resultsmentioning

confidence: 99%

“…14) Rationally designed acrylamide-based copolymers bind to small molecules, 15) peptides, 16) and proteins 17) with high selectivity by optimizing functional monomer structure and feed ratio. 18,19) It has been also reported that polymer flexibility and length are important for the induction of high target specificity and affinity. 20,21) Although these synthetic polymers can bind to target molecules with high affinity and specificity both in vitro and in vivo, they must be purified before use because they are synthesized using nonbiological monomers.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Purification Process on the Function of Synthetic Polymer Nanoparticles

Yasuno

Koide

Oku

et al. 2021

CHEMICAL & PHARMACEUTICAL BULLETIN

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Multifunctional synthetic polymers can bind to target molecules and are therefore widely investigated in diagnostics, drug delivery carriers, and separation carriers. Because these polymers are synthesized from nonbiological components, purification processes (e.g., chromatography, dialysis, extraction, and centrifugation) must be conducted after the synthesis. Although several purification methods are used for polymer purification, few reports have revealed the influence of purification process on the functions of polymer. In this study, we demonstrated that the characteristics, function, and stability of synthetic polymer depend on the purification process. N-Isopropylacrylamide-based polymer nanoparticles (NPs) and melittin (i.e., honey bee venom) were used as a model of synthetic polymer and target toxic peptide, respectively. Synthesized NPs were purified by dialysis in methanol, acetone precipitation, or centrifugation. NPs purified by dialysis in ultrapure water were used as control NPs. Then, NP size, surface charge, toxin neutralization effect, and stability were determined. NP size did not considerably change by purification with centrifugation; however, it decreased by purification using dialysis in methanol and acetone precipitation compared with that of control NPs. The ζ-potential of NPs changed after each purification process compared with that of control NPs. The melittin neutralization efficiency of NPs depended on the purification process; i.e., it decreased by acetone precipitation and increased by dialysis in methanol and centrifugation compared with that of control NPs. Of note, the addition of methanol and acetone decreased NP stability. These studies implied the importance of considering the effect of the purification method on synthetic polymer function.

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Section: Resultsmentioning

confidence: 99%