Design of experiments for the development of poly(d,l-lactide-co-glycolide) nanoparticles loaded with Uncaria tomentosa

Poly(lactic-co-glycolic acid) Nanoparticles Loaded with Callistemon citrinus Phenolics Exhibited Anticancer Properties against Three Breast Cancer Cell Lines

“…Sizes of PLGA nanoparticles loaded by berberine, C. citrinus extract, and mixtures of berberine and C. citrinus extract were 250.7 ± 0.06, 278.8 ± 0.007, and 274.8 ± 0.028 nm, respectively (Table 1). Nanoparticle shapes observed under SEM were polygonal (hexagonal) with diverse sizes, with unique morphological features (Figures 2(a) and 2(b)), as compared to a previously reported formulation [24]. Various studies have demonstrated that tailoring of the shape of nanoparticles could improve their effectiveness.…”

“…Similarly, blank samples of PLGA nanoparticles were synthesized without the tissue extracts by adding only PLGA solution following the same steps outlined previously. e PLGA nanoparticles were centrifuged (Eppendorf centrifuge 5415C, Hamburg, Germany) at 14,000 rpm for 15 minutes and then collected and purified by ultrafiltration as previously described [24,25]. PLGA nanoparticles were lyophilized by mixing synthesized nanoparticles with trehalose (EDM Chemicals, Philadelphia, PA, USA) at 1 : 1 ratio by pouring the PLGA nanoparticles into glass vials covered with a double layer of Parafilm.…”

Characterization and in vitro antitumor activity of polymeric nanoparticles loaded with Uncaria tomentosa extract

“…Polymeric nanoparticles were prepared by an oil-in-water single emulsion solvent evaporation method, based on optimized methods described previously (Ribeiro et al 2013(Ribeiro et al , 2015). An organic phase was prepared by dissolving polymer, either PLGA or PCL, in 5 mL of an ethyl acetate/acetone mixture at a 3:2 ratio containing UT extract with ~1.4 mg of total alkaloid content.…”

“…Another notable difference was that Np UT-PLGA had an ~20% higher total alkaloid concentration (20.0 μg/mL) compared to Np UT-PCL (16.5 μg/ mL) as well as a higher concentration of free alkaloids due to its lower entrapment efficiency. The total alkaloid concentration difference between the nanoparticle preparations was due in part to the aqueous phase final volume used for each nanoparticle, which was a consequence of the different optimal conditions achieved in the experimental designs (Ribeiro et al 2013(Ribeiro et al , 2015.…”

Optimization of protein loaded PLGA nanoparticle manufacturing parameters following a quality-by-design approach

“…13 DoE has been applied in the formulation optimization of different nanosystems, including metallic, 14,15 lipid, 16,17 and polymeric nanoparticles. 18,19 Poly(lactic-co-glycolic acid) (PLGA) is one of the most explored biocompatible and biodegradable synthetic polymer for the delivery of multiple bioactive molecules, being a component of medicines already approved by FDA and EMA for parenteral administration in humans. 20 This is the most studied biomaterial for drug delivery, presenting particularly attractive mechanical properties and tunable degradation rates, suitable for the release of the desired dose and release interval of hydrophilic and hydrophobic molecules.…”