Design of experiments (DOE)—history, concepts, and relevance to in vitro culture

Niedz, Randall P.; Evens, Terence J.

doi:10.1007/s11627-016-9786-1

Cited by 62 publications

(47 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The dimensionality of mixture designs can be expressed as n -1 dimensions where n is the number of components [ 22 ]. Therefore, the screening design of 8 ingredients is referred to as a 7-dimensional design space.…”

Section: Methodsmentioning

confidence: 99%

“…This traditional method to determine the effect of varying the doses of several components requires large factorial experiments resulting in a huge numbers of treatment combinations and parallel variation of variables, making it difficult to interpret the results [ 21 ]. However, the process of changing one-ingredient-at-a-time (OIAT) within a mixture is not capable of identifying an optimal blend of the ingredients because the effects of proportion and amount are confounded in factorial designs [ 22 ]. When the proportion of any ingredient is varied, the proportions of all remaining ingredients are also changed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae

et al. 2017

Self Cite

View full text Add to dashboard Cite

The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, is the most serious insect pest of corn (Zea mays L.) in the United States and parts of Europe, and arguably one of the world’s most expensive pests to control. Several diet formulations are currently used by industry and public researchers to evaluate WCR larvae in diet-toxicity bioassays. However, a publicly available diet that produces normative insects that are physiologically similar to WCR larvae reared on corn roots will accelerate development of management technologies. We report a new diet formulation that supports improved weight gain, larval development and survival compared with the only public diet for WCR that is currently available in the refereed literature. The formulation was created by using response surface methods combined with n-dimensional mixture designs to identify and improve the formulation of key ingredients. Weight gain increased two-fold, and survival and molting rates increased from 93% and 90%, respectively when reared on the public diet, to approximately 99% for both survival and molting at 11 days when reared on our new formulation. This new formulation provides a standardized growth medium for WCR larvae that will facilitate comparison of research results from various working groups and compliance with regulatory requirements.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…The design of experiments (DOE) provides researchers to program investigations by dramatically reducing the number of combinations to be studied and distributing them appropriately in the n -dimensional design space (Niedz and Evens, 2016). As a result, it is possible to obtain general conclusions, from the generated databases under statistically optimal conditions.…”

Section: Introductionmentioning

confidence: 99%

“…If we think that mineral nutrients are just a part of the plant tissue culture medium and that other components such as vitamins or plant growth regulators need also being added and optimized, it is clear that finding adequate composition for plant tissue culture media requires to deal with high dimensional spaces, which represent a great challenge for researchers (Niedz and Evens, 2016). In this situation, artificial intelligence tools are presented as promising candidates to extract information from the big databases that can be generated during the development of the culture media using high dimensional spaces (Gallego et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Combining DOE With Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio Rootstocks in vitro Culture

et al. 2018

View full text Add to dashboard Cite

The aim of this study was to determine the effects of Murashige and Skoog (MS) salts on optimal growth of two pistachio rootstocks, P. vera cv. “Ghazvini” and “UCB1” using design of experiments (DOE) and artificial intelligence (AI) tools. MS medium with 14 macro—and micro-elements was used as base point and its concentration varied from 0 to 5 × MS concentrations. Design of experiments (DOE) software was used to generate a five-dimensional design space by categorizing MS salts into five independent factors (NH4NO3, KNO3, mesos, micros and iron), reducing the experimental design space from 3,125 to just 29 treatments. Typical plant growth parameters such as shoot quality (SQ), proliferation rate (PR), shoot length (SL), and some physiological disorders including shoot-tip necrosis (STN) and callus formation at the base of explants (BC) were evaluated for each treatment. The results were successfully modeled using neurofuzzy logic software. The model delivered new insights, by different sets of “IF–THEN” rules, pinpointing the key role of some ion interactions (SO42- × Cl−, K+ × SO42- × EDTA−, and Fe2+ × Cu2+ × NO3-) for SQ, PR, and SL, whilst physiological disorders (STN and BC) were governed mainly by independent ions as Fe2+ and EDTA−, respectively. In our opinion, the methodology and results obtained in this study is extremely useful to understand the effect of mineral nutrients on pistachio in vitro culture, through discovering new complex interactions among macro—and micro-elements which can be implemented to design new media of plant tissue culture and improve healthy plant micropropagation for any plant species.

show abstract

“…In this study, a design of experiment (DoE) approach was utilized to examine parameters that affect plant regeneration and healthy root. Design of experiment is a statistical methodology that allows simultaneous testing of multiple factors to understand and improve complex systems (Condra 2001;Niedz and Evens 2016).…”

Section: Introductionmentioning

confidence: 99%

Use of DoE methodology to optimize the regeneration of high-quality, single-copy transgenic Zea mays L. (maize) plants

Chu

Adelberg

Lowe

et al. 2019

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

The maize Agrobacterium tumefaciens-mediated transformation process normally takes about 10 to 15 wk from embryo infection and co-cultivation to sending "T0" plants to the greenhouse (GH). A new method was developed using the maize transcription factors Babyboom (BBM) and Wuschel2 (WUS2), to stimulate direct transgenic embryo formation and plant regeneration, that bypasses the need for prolonged tissue culture and regeneration from callus. In the present study, a design of experiment (DoE) method was used to test 10 factors to optimize the quality of somatic embryo maturation, root formation, and subsequent plantlet survival, without compromising the molecular event quality. The concentration of NO 3 − and the ratio of NH 4 + to K + had significant effects on the morphology of plantlets derived directly from germinated transgenic embryos. During early development, optimal tissue morphology required a NH 4 + /K + ratio of 1:1 with 20 mM [NO 3 − ], of 14.2 μM 6-benzylaminopurine (BAP), the highest concentration tested, and a low light intensity of 50 μmol m −2 s −1. Later development of rooted shoots required additional macronutrients with reduced NH 4 NO 3 (15 mM NH 4 NO 3 and 25 mM KNO 3), reduced BAP (7.4 μM), and 1 μM abscisic acid (ABA) and at a higher light intensity of 140 μmol m −2 s −1. Using the optimized parameters, the frequency of plants sent to the GH was improved by twofold compared with the current process and the number of single-copy T-DNA events was doubled.

show abstract

Design of experiments (DOE)—history, concepts, and relevance to in vitro culture

Cited by 62 publications

References 34 publications

Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae

Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae

Combining DOE With Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio Rootstocks in vitro Culture

Use of DoE methodology to optimize the regeneration of high-quality, single-copy transgenic Zea mays L. (maize) plants

Contact Info

Product

Resources

About