Design of experiment and data analysis by JMP® (SAS institute) in analytical method validation

Ye, Christine; Liu, June; Ren, Feiyan; Okafo, Ngozi

doi:10.1016/s0731-7085(00)00335-6

Cited by 76 publications

(44 citation statements)

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“…For each gene sought via multiplex PCR, Fisher's exact test (two-tailed) was used to test the null hypothesis of equal proportions between populations, with step-down permutation multiplicity adjustments used to address the inflation of the type I error rate associated with large numbers of tests (45). Hierarchical clustering analysis was performed using JMP 7.0 to identify correlations between sequenced genomes based upon APEC O1 island prevalences, between the groups (source group, phylogenetic group, and serogroup) examined based upon gene prevalences, and between the genes sought among the isolates examined (46). Differences in mean status values for the mouse sepsis model were tested for statistical significance using the Mann-Whitney U test, where a P value of Ͻ0.05 was considered significant.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Avian-Pathogenic Escherichia coli Strain O1 Genomic Islands among Extraintestinal and Commensal E. coli Isolates

et al. 2012

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Escherichia coli strains that cause disease outside the intestine are known as extraintestinal pathogenic E. coli (ExPEC) and include pathogens of humans and animals. Previously, the genome of avian-pathogenic E. coli (APEC) O1:K1:H7 strain O1, from ST95, was sequenced and compared to those of several other E. coli strains, identifying 43 genomic islands. Here, the genomic islands of APEC O1 were compared to those of other sequenced E. coli strains, and the distribution of 81 genes belonging to 12 APEC O1 genomic islands among 828 human and avian ExPEC and commensal E. coli isolates was determined. Multiple islands were highly prevalent among isolates belonging to the O1 and O18 serogroups within phylogenetic group B2, which are implicated in human neonatal meningitis. Because of the extensive genomic similarities between APEC O1 and other human ExPEC strains belonging to the ST95 phylogenetic lineage, its ability to cause disease in a rat model of sepsis and meningitis was assessed. Unlike other ST95 lineage strains, APEC O1 was unable to cause bacteremia or meningitis in the neonatal rat model and was significantly less virulent than uropathogenic E. coli (UPEC) CFT073 in a mouse sepsis model, despite carrying multiple neonatal meningitis E. coli (NMEC) virulence factors and belonging to the ST95 phylogenetic lineage. These results suggest that host adaptation or genome modifications have occurred either in APEC O1 or in highly virulent ExPEC isolates, resulting in differences in pathogenicity. Overall, the genomic islands examined provide targets for further discrimination of the different ExPEC subpathotypes, serogroups, phylogenetic types, and sequence types.

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Section: Methodsmentioning

confidence: 99%

Prevalence of Avian-Pathogenic Escherichia coli Strain O1 Genomic Islands among Extraintestinal and Commensal E. coli Isolates

et al. 2012

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“…An effective method to investigate the impact of the factors on the process response is to develop a second-order regression model that includes both the linear and quadratic terms [19]. It is possible to include cubic or even higher-order terms; however this approach requires a considerably larger number of experiments for model-fitting.…”

Section: Statistical Designmentioning

confidence: 99%

“…It is possible to include cubic or even higher-order terms; however this approach requires a considerably larger number of experiments for model-fitting. The adoption of a second-order model may also be justified by the observation that high-order terms typically contribute insignificantly to the process response in practice [19].…”

Section: Statistical Designmentioning

confidence: 99%

“…The contour profiler is an interactive facility used to optimize response surface graphically. The three dimensional response surfaces and contour plots were used to facilitate a straightforward examination of the influence of experimental variables on the responses [16,17,19]. Each response surface and contour plot was created by keeping two out of five variables constant at their centre points.…”

Section: Contour Profilersmentioning

confidence: 99%

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Statistical Modelling and Analysis of the Aerobic Oxidation of Benzyl Alcohol over K–Mn/C Catalysts

et al. 2008

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We report the application of statistical multivariate analysis to optimize the process parameters (including temperature, O 2 pressure, concentration of benzyl alcohol, Mn content, and K:Mn molar ratio) in the oxidation of benzyl alcohol using molecular oxygen over K-Mn/C catalysts. This multi-regression model, based on 38 experimental runs, shows reasonably accurate prediction on the benzyl alcohol conversion and turnover frequency (TOF). Moreover, the synergistic effects between reaction parameters are revealed, in particular, it is found that the benzyl alcohol conversion and TOF are in positive correlation with temperature, O 2 pressure and K:Mn molar ratio, with temperature having the most significant impact. In contrast, the concentration of benzyl alcohol and Mn content affect the conversion and TOF in a complex and opposite way. We hope to achieve a comprehensive understanding of the reaction process and provide valuable guidance for industrial scale-up of the reaction with the objective of optimal operations.

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“…14, 15 Ye et al 16 have reported the applicability of DOE and data treatment by JMP ® and Hibbert 17 has reviewed the applicability of DOE in chromatography. In recent years the applicability of DOE and statistical data treatment of years, HPLC data have increased immensely.…”

Section: Introductionmentioning

confidence: 99%

Experimental design approach to optimize stability indicating LC method for the determination of Naftopidil in its bulk and tablet dosage form

kumar¹,

Spandana²,

Shantikumar³

et al. 2014

J Young Pharm.

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Objective: To develop and validated a new stability indicating reverse phase high performance liquid chromatography (RP-HPLC) method for analysis of naftopidil (NAF), both as a bulk drug and in formulation. Method: The separation was achieved using a C18 GRACE column (250 mm × 4.6 mm i.d., 5 μm particle size) and gradient mobile phase system consisting of (A) 10 mM of ammonium acetate buffer pH adjusted to 4.0 with glacial acetic acid and (B) acetonitrile. The fl ow rate was 1.0 mL/ min with UV detection at 284 nm. NAF was subjected to stress conditions like hydrolysis (acid, alkali and neutral degradation), oxidation, photolytic and thermal decomposition. The linearity of the proposed method was investigated in the range of 10-150 μg/mL. Application of design of experiments for the robustness study method was carried out, where in fi ve factors was selected: pH of mobile phase, fl ow rate, strength of the buffer and column temperature. These factors were examined using JMP@ (SAS Institute) software. Result: The analytical method for NAF was developed and validated at the linearity range of 10-150 μg/mL. The LOD and LOQ were 0.6 and 2.04, respectively and accuracy of analysis was 100.5-101.1%. Conclusion: A robuststabilityindicating HPLC assay method was developed using DOE, for the quantitation of NAF in its bulk and tablet dosage forms

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Design of experiment and data analysis by JMP® (SAS institute) in analytical method validation

Cited by 76 publications

References 0 publications

Prevalence of Avian-Pathogenic Escherichia coli Strain O1 Genomic Islands among Extraintestinal and Commensal E. coli Isolates

Prevalence of Avian-Pathogenic Escherichia coli Strain O1 Genomic Islands among Extraintestinal and Commensal E. coli Isolates

Statistical Modelling and Analysis of the Aerobic Oxidation of Benzyl Alcohol over K–Mn/C Catalysts

Experimental design approach to optimize stability indicating LC method for the determination of Naftopidil in its bulk and tablet dosage form

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