Design of Epitope Based Peptide Vaccine Against Pseudomonas Aeruginosa Fructose Bisphosphate Aldolase Protein using Immunoinformatics

Abstract

Pseudomonas aeruginosa is common pathogen that is responsible of serious illnesses hospital acquired infection as ventilator associated pneumonia and various sepsis syndrome. Also it is a multidrug resistant pathogen recognized for its ubiquity, its intrinsically advanced antibiotic resistant mechanisms. generally affects the immuonocompromised but can also infect the immunocompetent as in hot tub folliculitis. There is no vaccine against it available till now. This study predicts an effective epitope-based vaccine against Fructose bisphosphate aladolase (FBA) of Pseudomonas aeruginosa using immunoinformatics tools. The sequences were obtained from NCBI and prediction tests took place to analyze possible epitopes for B and T cells. Three B cell epitopes passed the antigenicity, accessibility and hydrophilicity tests. Six MHC I epitopes were the most promising, while four from MHC II. Nineteen epitopes were shared between MHC I and II. For the population coverage, the epitopes covered 95.62% of the alleles worldwide excluding certain MHC II alleles. We recommend invivo and invitro studies to prove it's effectiveness.

Multifunctional Fructose 1,6-Bisphosphate Aldolase as a Therapeutic Target

“…Aldolase vaccination studies involving bacteria, fungi, or parasites all invariably resulted in the development of varying degrees of protection from challenge infection, and this was associated with induced cellular and/or humoral immune responses. Novel immunoinformatics approaches to predict optimal peptide sequences with strong binding affinities to host MHC molecules while minimizing allergic reactivity may prove useful in simplifying the search for specific antigenic epitopes within the enzyme structure ( Mohammed et al, 2018 ; Elhag et al, 2020 ). The development of new technologies such as immunoinformatics, the exploration of new vaccine designs and alternative routes of immunogen administration (i.e., protein versus DNA versus RNA, oral administration versus intramuscular etc.…”

Epitope-Based Peptide Vaccine Design against Fructose Bisphosphate Aldolase of Candida glabrata: An Immunoinformatics Approach

“…A previous study conducted by de Klerk et al [79] showed that the Fba1 protein has the ability to provoke immune responses in human against M. mycetomatis [79]. Also, several recent publications have used the Fba1 protein as a strong antigenic target for predicting Band T-cell epitopes in order to design promising vaccines against fungal and bacterial pathogens such as M. mycetomatis, P. aeruginosa, L. monocytogenes, and S. mansoni by using in silico tools [80][81][82][83]. Hence, there are more studies to explore the fructose bisphosphate aldolase protein immunogenic role and the possibility to find common conserved epitopes for different organisms.…”