Design of electrochemical immunosensors using electro-click chemistry. Application to the detection of IL-1β cytokine in saliva

Guerrero, Sara; Agüı́, L.; Yáñez‐Sedeño, P.; Pingarrón, José M.

doi:10.1016/j.bioelechem.2020.107484

Cited by 39 publications

(29 citation statements)

References 32 publications

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“…Furthermore, the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) has a great variety of possibilities in electrochemical biosensors [73]. To overcome the long time needed for chemical generation of the cuprous ion (~16 h), the electrogeneration of the catalyst by applying a previously optimized reduction potential for a fixed time (~300 s) has been exploited, leading to a methodology known as electroclick chemistry that provides good results in the preparation of biosensors [59,74].…”

Section: Integrated Biosensors Involving Surface Chemistries For the mentioning

confidence: 99%

“…Table 2 shows that both diazonium salts-based electrografting and click and electroclick chemistries have been used for the construction of electrochemical biosensors for inflammatory cytokines. These methods involved the functionalization of carbon nanomaterials [27,[41][42][43]52] and electrode surfaces [11,13,14,26,36,43,46,52,54,55,[57][58][59][60][61] or the incorporation of ethynyl-functionalized antibodies [41,57,59].…”

Section: Integrated Biosensors Involving Surface Chemistries For the mentioning

confidence: 99%

“…This strategy allowed loading a large amount of CAb and antifouling 4aminophenyl phosphorylcholine (PPC) molecules (Au/RGO-ph-AuNP-PPC(-ph-COOH)/CAb) as well as the use of GO nanocomposites modified with DAb and 4-ferrocenylaniline (DAb-GOph-Fc) as tracers (Figure 5). Moreover, immunoscaffolds reported for the determination of inflammatory cytokines and chemokines involved in click [42,57] or electro-click (Figure 6) [59] chemistry to prepare IgGs-MWCNTs by reaction of azide-functionalized MWCNTs and ethynyl-IgGs. The electrochemical immunoplatforms prepared using electrografting and click or electro-click chemistry exhibited analytical characteristics compatible with clinical applications providing LODs in the low pg mL −1 -ng mL −1 level, and were employed to perform Moreover, immunoscaffolds reported for the determination of inflammatory cytokines and chemokines involved in click [42,57] or electro-click (Figure 6) [59] chemistry to prepare IgGs-MWCNTs by reaction of azide-functionalized MWCNTs and ethynyl-IgGs.…”

Section: Figure 1 (A) Sandwich Immunoassay Involving Covalent and Tamentioning

confidence: 99%

“…Moreover, immunoscaffolds reported for the determination of inflammatory cytokines and chemokines involved in click [42,57] or electro-click (Figure 6) [59] chemistry to prepare IgGs-MWCNTs by reaction of azide-functionalized MWCNTs and ethynyl-IgGs. The electrochemical immunoplatforms prepared using electrografting and click or electro-click chemistry exhibited analytical characteristics compatible with clinical applications providing LODs in the low pg mL −1 -ng mL −1 level, and were employed to perform Moreover, immunoscaffolds reported for the determination of inflammatory cytokines and chemokines involved in click [42,57] or electro-click (Figure 6) [59] chemistry to prepare IgGs-MWCNTs by reaction of azide-functionalized MWCNTs and ethynyl-IgGs. Moreover, immunoscaffolds reported for the determination of inflammatory cyto kines and chemokines involved in click [42,57] or electro-click (Figure 6) [59] chemistry t prepare IgGs-MWCNTs by reaction of azide-functionalized MWCNTs and ethynyl-IgGs The electrochemical immunoplatforms prepared using electrografting and click o electro-click chemistry exhibited analytical characteristics compatible with clinical appl cations providing LODs in the low pg mL −1 -ng mL −1 level, and were employed to perform The electrochemical immunoplatforms prepared using electrografting and click or electro-click chemistry exhibited analytical characteristics compatible with clinical applications providing LODs in the low pg mL −1 -ng mL −1 level, and were employed to perform the analysis of the target cytokines/receptors (TNF-α, APN, TGF-β1, IL-6, IL-13Rα2, IFN-γ, RANKL, IL-1β and IL-10) in different human biofluids (saliva, serum, plasma and blood).…”

Section: Figure 1 (A) Sandwich Immunoassay Involving Covalent and Tamentioning

confidence: 99%

“…Sandwich immunoplatform for the DPV determination of IL-1β using IgGs-MWCNTs prepared by electro-click chemistry as SPCE modifiers. Reproduced from[59] with permission.…”

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confidence: 99%

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Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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The multifaceted key roles of cytokines in immunity and inflammatory processes have led to a high clinical interest for the determination of these biomolecules to be used as a tool in the diagnosis, prognosis, monitoring and treatment of several diseases of great current relevance (autoimmune, neurodegenerative, cardiac, viral and cancer diseases, hypercholesterolemia and diabetes). Therefore, the rapid and accurate determination of cytokine biomarkers in body fluids, cells and tissues has attracted considerable attention. However, many currently available techniques used for this purpose, although sensitive and selective, require expensive equipment and advanced human skills and do not meet the demands of today’s clinic in terms of test time, simplicity and point-of-care applicability. In the course of ongoing pursuit of new analytical methodologies, electrochemical biosensing is steadily gaining ground as a strategy suitable to develop simple, low-cost methods, with the ability for multiplexed and multiomics determinations in a short time and requiring a small amount of sample. This review article puts forward electrochemical biosensing methods reported in the last five years for the determination of cytokines, summarizes recent developments and trends through a comprehensive discussion of selected strategies, and highlights the challenges to solve in this field. Considering the key role demonstrated in the last years by different materials (with nano or micrometric size and with or without magnetic properties), in the design of analytical performance-enhanced electrochemical biosensing strategies, special attention is paid to the methods exploiting these approaches.

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Section: Integrated Biosensors Involving Surface Chemistries For the mentioning

confidence: 99%

Section: Integrated Biosensors Involving Surface Chemistries For the mentioning

confidence: 99%

Section: Figure 1 (A) Sandwich Immunoassay Involving Covalent and Tamentioning

confidence: 99%

Section: Figure 1 (A) Sandwich Immunoassay Involving Covalent and Tamentioning

confidence: 99%

“…Sandwich immunoplatform for the DPV determination of IL-1β using IgGs-MWCNTs prepared by electro-click chemistry as SPCE modifiers. Reproduced from[59] with permission.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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Biosensors for Salivary Biomarker Detection of Cancer and Neurodegenerative Diseases

Sajeevan,

Gopika,

Sreelekshmi

et al. 2023

Biosensors Nanotechnology

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Advances in Electrochemical Biosensors Based on Nanomaterials for Protein Biomarker Detection in Saliva

et al. 2022

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The focus on precise medicine enhances the need for timely diagnosis and frequent monitoring of chronic diseases. Moreover, the recent pandemic of severe acute respiratory syndrome coronavirus 2 poses a great demand for rapid detection and surveillance of viral infections. The detection of protein biomarkers and antigens in the saliva allows rapid identification of diseases or disease changes in scenarios where and when the test response at the point of care is mandated. While traditional methods of protein testing fail to provide the desired fast results, electrochemical biosensors based on nanomaterials hold perfect characteristics for the detection of biomarkers in point-of-care settings. The recent advances in electrochemical sensors for salivary protein detection are critically reviewed in this work, with emphasis on the role of nanomaterials to boost the biosensor analytical performance and increase the reliability of the test in human saliva samples. Furthermore, this work identifies the critical factors for further modernization of the nanomaterial-based electrochemical sensors, envisaging the development and implementation of next-generation sample-in-answer-out systems.

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Design of electrochemical immunosensors using electro-click chemistry. Application to the detection of IL-1β cytokine in saliva

Cited by 39 publications

References 32 publications

Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

Biosensors for Salivary Biomarker Detection of Cancer and Neurodegenerative Diseases

Advances in Electrochemical Biosensors Based on Nanomaterials for Protein Biomarker Detection in Saliva

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