Because USB has many advantages, such as plug and play, hot plug, so it has brought great changes in PC peripheral devices' connecting technology. In this paper, we firstly have designed a Microprocessor Minimum System which based on ARM7 kernel Microprocessor S3C44BOX. And on the basis of the Minimum System, we have designed a kind of data acquisition card which based on USB interface. And a kind of Embedded Operating System Jl C/OS-II also has been transplanted in the data acquisition, we also have used Jl C/OS-II to manage the whole data acquisition system. The whole data acquisition system's design includes hardware circuits' design and data acquisition interrupt software's design. After being used and examined practically, the USB Data Acquisition Card takes on such advantages as good performance, low consumption and low cost, especially for its use value. And it can be embedded in many kinds of data acquisition applications as a basic module.