Design of double-walled carbon nanotubes for biomedical applications

Neves, Vera; Heister, Elena; Costa, Staël de Alvarenga Pereira; Tîlmaciu, Carmen; Flahaut, Emmanuel; Soula, Brigitte; Coley, Helen M.; McFadden, Johnjoe; Silva, S. Ravi P.

doi:10.1088/0957-4484/23/36/365102

Cited by 47 publications

(24 citation statements)

References 28 publications

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“…Delivery of siRNA in vitro by surface-modified SWNTs was demonstrated in breast cancer cells [70]. Similar results were reported in various other cancer cell lines with knockdown of mRNA reaching around 40% and protein levels were reduced by 70% [63,67,68,79]. …”

Section: Gene Deliverysupporting

confidence: 64%

“…Multiple studies have explored different methods of modifying the surface of CNT for the improved delivery of nucleic acids, including pDNA [60–63], siRNA [9,53,60–68] and miRNA [14], into mammalian cells.…”

Section: Gene Deliverymentioning

confidence: 99%

“…Many studies have reported that CNTs are viable platforms for delivering biologically active siRNA into cells [9,53,64–68,70]. Much of the earlier research using CNT-mediated siRNA delivery focused on silencing genes that suppressed the proliferation and growth of cancer cells, but other therapeutic applications of CNTs have been reported to transfect difficult cell types [77] such as skeletal muscle cells [65] and T cells [78].…”

Section: Gene Deliverymentioning

confidence: 99%

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Carbon nanotubes part II: a remarkable carrier for drug and gene delivery

Karimi

Solati

Ghasemi

et al. 2015

Expert Opinion on Drug Delivery

159

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Introduction Carbon nanotubes (CNT) have recently been studied as novel and versatile drug and gene delivery vehicles. When CNT are suitably functionalized, they can interact with various cell types and are taken up by endocytosis. Areas covered Anti-cancer drugs cisplatin and doxorubicin have been delivered by CNT, as well as methotrexate, taxol and gemcitabine. The delivery of the antifungal compound amphotericin B and the oral administration of erythropoietin have both been assisted using CNT. Frequently, targeting moieties such as folic acid, epidermal growth factor or various antibodies are attached to the CNT-drug nanovehicle. Different kinds of functionalization (e.g., polycations) have been used to allow CNT to act as gene delivery vectors. Plasmid DNA, small interfering RNA and micro-RNA have all been delivered by CNT vehicles. Significant concerns are raised about the nanotoxicology of the CNT and their potentially damaging effects on the environment. Expert opinion CNT-mediated drug delivery has been studied for over a decade, and both in vitro and in vivo studies have been reported. The future success of CNTs as vectors in vivo and in clinical application will depend on achievement of efficacious therapy with minimal adverse effects and avoidance of possible toxic and environmentally damaging effects.

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Section: Gene Deliverysupporting

confidence: 64%

Section: Gene Deliverymentioning

confidence: 99%

Section: Gene Deliverymentioning

confidence: 99%

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Carbon nanotubes part II: a remarkable carrier for drug and gene delivery

Karimi

Solati

Ghasemi

et al. 2015

Expert Opinion on Drug Delivery

159

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“…Although their production remains lower than that of MWCNTs, DWCNTs present great interest to high-tech industries that supply nanoprobes or develop composite reinforcements, energy storage media, displays, touch screens, and other electronic devices [4]. They are also of particular interest for biomedical applications [5,6]. Considering their increased uses, sooner or later CNTs will be present in substantial concentrations in the environment [7] and especially in aquatics ecosystems, which are known to concentrate many contaminants.…”

Section: Introductionmentioning

confidence: 99%

Double walled carbon nanotubes promote the overproduction of extracellular protein-like polymers in Nitzschia palea: An adhesive response for an adaptive issue

Verneuil

Silvestre

Randrianjatovo

et al. 2015

Carbon

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A B S T R A C TThe present study assessed the effects of double-wall carbon nanotubes (DWCNTs) dispersed in the presence of a realistic concentration of natural organic matter (NOM, 10 mg L À1 ) on the benthic diatom Nitzschia palea using toxicity tests and quantitative/qualitative extracellular polymeric substances (EPS) assays. No toxic effect was observed. A growth delay was measured after 48 h of exposure to concentrations of DWCNTs ranging from 1 mg L À1 ($29%) to 50 mg L À1 ($84%). Extracellular carbohydrates and proteins were extracted using a sequential multi-methods protocol to collect soluble, hydrophobic and ion-bridged extracellular polymeric substances (EPS). Extracted EPS were analyzed by colorimetric assays and size exclusion chromatography. The results highlighted a higher EPS concentration in exposed cultures that was primarily caused by an overproduction of protein-like polymers (protein or glycoproteins, PLPs). Such EPS overproduction and increase in proteins/carbohydrates ratio can partially explain the observed growth inhibition. EPS were preferentially extracted using hydrophobic conditions and were mainly composed of PLPs with either low (10 kDa) or high (174 kDa) molecular weights. These data highlights the affinity between DWCNTs and EPS, which is primarily driven by both physical and hydrophobic interactions. This indicates that N. palea can respond to DWCNTs by forming an EPS network optimized for adhering to and efficiently wrapping DWCNTs.

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“…Attachment may be established directly to the core of the nanoparticle [38,82] or through intermediates, such as polymers [83,84], dendrimers [79,85] and short linkers [18,86], among others.…”

Section: Functionalization Of Inorganic Nanoparticles With Sirnamentioning

confidence: 99%

15 years on siRNA delivery: Beyond the State-of-the-Art on inorganic nanoparticles for RNAi therapeutics

et al. 2015

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RNAi has always captivated scientists due to its tremendous power to modulate the phenotype of living organisms. This natural and powerful biological mechanism can now be harnessed to downregulate specific gene expression in diseased cells; opening up endless opportunities. Since most of the conventional siRNA delivery methods are limited by a narrow therapeutic index and significant side and off-target effects, we are now in the dawn of a new age in gene therapy driven by nanotechnology vehicles for RNAi therapeutics. Here, we outlook the "do's and dont's" of the 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 REVIEW NANO TODAY 2 inorganic RNAi nanomaterials developed in the last 15 years and the different strategies employed are compared and scrutinized, offering important suggestions for the next 15. *Manuscript Click here to view linked References

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Design of double-walled carbon nanotubes for biomedical applications

Cited by 47 publications

References 28 publications

Carbon nanotubes part II: a remarkable carrier for drug and gene delivery

Carbon nanotubes part II: a remarkable carrier for drug and gene delivery

Double walled carbon nanotubes promote the overproduction of extracellular protein-like polymers in Nitzschia palea: An adhesive response for an adaptive issue

15 years on siRNA delivery: Beyond the State-of-the-Art on inorganic nanoparticles for RNAi therapeutics

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