Design of disposable DNA biosensor microchip with amperometric detection featuring PCB substrate

Ghanim, Motasem H.; Abdullah, Mohd Zaid

doi:10.1007/s13206-013-7108-9

Cited by 17 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results showed in Figure (a) indicate that the cultured fibroblasts adhered to the on all the scaffolds and proliferated as the respected optical density increased through time . In addition, the attachment and morphology of L929 cells were examined for the cells cultured on P2S1 dressing after 7‐day incubation as shown in Figure (b).…”

Section: Resultsmentioning

confidence: 99%

Sustained release of CIP from TiO₂‐PVDF/starch nanocomposite mats with potential application in wound dressing

Ansarizadeh

Haddadi

Amini

et al. 2020

J of Applied Polymer Sci

View full text Add to dashboard Cite

Electrospinning is an economical and alluring method to fabricate wound dressing mats from a variety of natural and synthetic materials. In this study, polyvinylidene fluoride (PVDF) and starch composite mats containing ciprofloxacin (CIP) loaded on titanium dioxide nanoparticles (TiO 2 ) were prepared. Fourier Transform Infrared spectra of CIP, synthesized TiO 2 NPs, TiO 2 /CIP, and PVDF/starch composite mats are analyzed. Scanning electron microscopy images revealed that the fiber diameter of PVDF nanofibers thickens by increasing starch, which varies between 180 nm and 550 nm. Strain at break of PVDF, starch, PVDF/starch (2:1 w:w; P2S1), PVDF/starch (1:1 w:w; P1S1), PVDF/starch (1:2 w:w; P1S2), and nanofibers were 103 AE 11, 3 AE 0.6, 27 AE 4, 52 AE 5.2, 7.7 AE 1%, respectively. Based on strain at break and young modulus, P2S1 was selected as a suitable candidate for wound dressing to which load TiO 2 / CIP as a bioactive agent. The release rate of CIP showed that about 40% of the drug is released in the first 2 days. Furthermore, the antibacterial activity of dressings was investigated using Escherichia coli and Staphylococcus aureus microorganisms and zones of clearance were obvious around the specimen on the agar plate.

show abstract

Section: Resultsmentioning

confidence: 99%

Sustained release of CIP from TiO₂‐PVDF/starch nanocomposite mats with potential application in wound dressing

Ansarizadeh

Haddadi

Amini

et al. 2020

J of Applied Polymer Sci

View full text Add to dashboard Cite

show abstract

“…Therefore, both electropherograms contained cathodic peaks only. (13) Figure 4 shows the individual current responses produced during amperometric detection using (a) Pt and (b) Cu as the working electrodes. For both experiments, the separation time was 10 min, and the separation voltage was 125 V corresponding to an electric field strength of 25 V/cm.…”

Section: Resultsmentioning

confidence: 99%

“…In this case, Pt is selected as an electrode material because of its unique features, such as good electrical conductivity and inertness to redox reactions. (12,13) The design is based on an in-channel amperometric detection technique and consists of three Pt/Cu sensing electrodes, namely, working, reference, and counter electrodes. Figure 2 shows the procedure for fabricating the microchip consisting of PDMS and glass substrates.…”

Section: Fabrication Materialsmentioning

confidence: 99%

Design of a Low-Cost DNA Biochip Using Copper Electrode

2015

Sensors and Materials

View full text Add to dashboard Cite

The need for affordable and disposable DNA biochips that can perform in situ analysis is increasing daily. Amperometric detection (AD) is one of the most popular detection techniques used in DNA sensing. An important component of these biochips is the working electrode that needs to be carefully designed to achieve good sensitivity. At present, most AD biochips use platinum (Pt) or gold (Au), since these materials are not only sensitive but also produce good signal-to-noise ratios. Hence, the cost of fabrication is a major factor since Pt or Au requires expensive machining technologies. As a tradeoff between cost and sensitivity, in this study, copper is used as an alternative material in designing the biochips. DNA sensing is performed using newly designed biochips, and the performance is compared with those from a Pt electrode. Results from this study suggest the feasibility of designing not only low-cost but truly disposable biochips for DNA sensing.

show abstract

“…In such hybrid devices, integration of Si transducer chips (for conductivity-based or pH detection) has been also made possible together with microfluidics fabricated in PDMS. 100 Disposable PDMS-PCB chips were also reported for electrophoretic separation and amperometric DNA detection 101 or low cost detection and enumeration of circulating tumor cells in a microfluidic impedance cytometer. 102,103 In addition, highly integrated microfluidic components and LoC devices have been developed on PCB substrates for on-chip sample preparation, DNA amplification and detection, by integrating multiple embedded heating and sensing elements together with electrical circuitries on a PCB with a polymeric cover where the microfluidic network (mixers, valves, pumps, etc.)…”

Section: Technology Overview and Demonstrated Prototypesmentioning

confidence: 99%

The lab-on-PCB approach: tackling the μTAS commercial upscaling bottleneck

2017

View full text Add to dashboard Cite

Commercialization of lab-on-a-chip devices is currently the "holy grail" within the μTAS research community. While a wide variety of highly sophisticated chips which could potentially revolutionize healthcare, biology, chemistry and all related disciplines are increasingly being demonstrated, very few chips are or can be adopted by the market and reach the end-users. The major inhibition factor lies in the lack of an established commercial manufacturing technology. The lab-on-printed circuit board (lab-on-PCB) approach, while suggested many years ago, only recently has re-emerged as a very strong candidate, owing to its inherent upscaling potential: the PCB industry is well established all around the world, with standardized fabrication facilities and processes, but commercially exploited currently only for electronics. Owing to these characteristics, complex μTASs integrating microfluidics, sensors, and electronics on the same PCB platform can easily be upscaled, provided more processes and prototypes adapted to the PCB industry are proposed. In this article, we will be reviewing for the first time the PCB-based prototypes presented in the literature to date, highlighting the upscaling potential of this technology. The authors believe that further evolution of this technology has the potential to become a much sought-after standardized industrial fabrication technology for low-cost μTASs, which could in turn trigger the projected exponential market growth of μTASs, in a fashion analogous to the revolution of Si microchips via the CMOS industry establishment.

show abstract

Design of disposable DNA biosensor microchip with amperometric detection featuring PCB substrate

Cited by 17 publications

References 12 publications

Sustained release of CIP from TiO₂‐PVDF/starch nanocomposite mats with potential application in wound dressing

Sustained release of CIP from TiO₂‐PVDF/starch nanocomposite mats with potential application in wound dressing

Design of a Low-Cost DNA Biochip Using Copper Electrode

The lab-on-PCB approach: tackling the μTAS commercial upscaling bottleneck

Contact Info

Product

Resources

About

Design of disposable DNA biosensor microchip with amperometric detection featuring PCB substrate

Cited by 17 publications

References 12 publications

Sustained release of CIP from TiO2‐PVDF/starch nanocomposite mats with potential application in wound dressing

Sustained release of CIP from TiO2‐PVDF/starch nanocomposite mats with potential application in wound dressing

Design of a Low-Cost DNA Biochip Using Copper Electrode

The lab-on-PCB approach: tackling the μTAS commercial upscaling bottleneck

Contact Info

Product

Resources

About

Sustained release of CIP from TiO₂‐PVDF/starch nanocomposite mats with potential application in wound dressing

Sustained release of CIP from TiO₂‐PVDF/starch nanocomposite mats with potential application in wound dressing