Design of Curcumin loaded Cellulose Nanoparticles for Prostate Cancer

Abstract

Prostate cancer (PC) is the most frequently diagnosed disease in men in the United States. Curcumin (CUR), a natural polyphenol, has shown potent anti-cancer efficacy in various types of cancers. However, suboptimal pharmacokinetics and poor bioavailability limit its effective use in cancer therapeutics. Several successful CUR nanoformulations have recently been reported which improve upon these features; however there is no personalized safe nanoformulation for prostate cancer. This study contributes two important scientific aspects of prostate cancer therapeutics. The first objective was to investigate the comparative cellular uptake and cytotoxicity evaluation of β-cyclodextrin (CD), hydroxyl propyl methyl cellulose (cellulose), poly(lactic-co-glycolic acid) (PLGA), magnetic nanoparticles (MNP), and dendrimer based CUR nanoformulations in prostate cancer cells. Curcumin loaded cellulose nanoparticles (cellulose-CUR) formulation exhibited the highest cellular uptake and caused maximum ultrastructural changes related to apoptosis (presence of vacuoles) in prostate cancer cells. Secondly, the anti-cancer potential of the optimized cellulose-CUR formulation was evaluated in cell culture models using cell proliferation, colony formation and apoptosis (7-AAD staining) assays. In these assays, the cellulose-CUR formulation showed improved anti-cancer efficacy compared to free curcumin. Our study shows, for the first time, the feasibility of cellulose-CUR formulation and its potential use in prostate cancer therapy.

Curcumin-Loaded Nanoparticles Protect Against Rhabdomyolysis-Induced Acute Kidney Injury

“…Recently, various nano formulations of curcumin have been synthesized to overcome these issues. Nanoparticle-loaded curcumin appeared to achieve high permeability, good bioactivity and low metabolism in animal models [25-27]. In the present study, the nanomaterial distearoylphosphatidyethanolamin-polyethylene glycol (DSPE-PEG) was employed as a nanocarrier for curcumin.…”

Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes

“…Curcumin nanoformulations ( Figure 1B) were obtained following our previous protocols using PLGA, β-cyclodextrin, cellulose, nanogel (polyNIPAM), and dendrimer through self-assembly/ complexation/redox free radical solution polymerization or encapsulation techniques. 21,22,26,36 For consistency, all curcumin nanoformulations were processed with 20 wt% curcumin loading. A schematic representation of curcumin nanoformulations are provided in Figure 1B.…”

“…16,[23][24][25] In our previous study, we describe a comparative evaluation of β-cyclodextrin, hydroxyl propyl methyl cellulose (cellulose), polylactic-co-glycolic acid (PLGA) and dendrimer curcumin nanoformulations with free curcumin using cytotoxicity, cellular uptake, and apoptosis studies in cancer cells and suggests a close relationship with the therapeutic efficacy of the formulation. 26 However, there is no tool to evaluate the behavior of curcumin nanoformulations in vivo. Similarly, while a number of curcumin nanoformulations have been reported in the literature, so far there has been no comparative study to evaluate which formulation(s) is more efficient for in vivo cancer therapeutics.…”

HPMA-based polymeric micelles for curcumin solubilization and inhibition of cancer cell growth

“…It was found that HPMA-based polymeric micelles 41 significantly enhanced the solubility of CM. The PEG-HPMA-Bz micelles showed the best solubilization 42 properties. CM loaded polymeric micelles showed sustained release of the loading CM for more than 43 20 days.…”