Design of Collagen-Based Hemostatic Material for Use in Plastic and Reconstructive Surgery

Kuroyanagi, Yoshimitsu; Suzuki, Ryusuke; Kuroyanagi, Misato

doi:10.4236/ojrm.2022.111002

Cited by 2 publications

(3 citation statements)

References 13 publications

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“…The sheet-shaped Col sponge was manufactured by the method described in our previous paper [17] [18]. The method was briefly described below.…”

Section: Manufacture Of Col Spongementioning

confidence: 99%

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Design of Novel Wound Dressing Composed of Collagen and Hyaluronic Acid Containing Epidermal Growth Factor

Kuroyanagi¹,

Suzuki²,

Kuroyanagi³

2023

OJRM

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This research aims to develop a wound dressing composed of collagen (Col) and hyaluronic acid (HA) containing epidermal growth factor (EGF). First important issue is to contain EGF in the wound dressing in a stable state. The sheet-shaped sponge was manufactured by freeze-vacuum drying an aqueous solution of Col. Both sides of sponge were treated with ultraviolet (UV) irradiation to introduce intermolecular cross links between collagen molecules. This sponge was named Sponge-Col. Another sheet-shaped sponge was manufactured by freeze-vacuum drying an aqueous solution of HA containing EGF. This sponge was named Sponge-HA/EGF. The wound dressing was manufactured by laminating Sponge-Col on the top, Sponge-HA/EGF in the middle, and Sponge-Col on the bottom to create a sandwich structure. This method can prevent the reducing of EGF activity due to UV irradiation for intermolecular cross-linking. Second important issue is to enable gradual release of EGF from the wound dressing. The elution behavior of this wound dressing was investigated by measuring the weight change after immersion in water for a predetermined time. This wound dressing showed initially fast elution and subsequent very slow elution properties. The upper layer and lower layer Sponge-Col enabled gradual release of the middle layer Sponge-HA/EGF. This result suggests that EGF contained in the wound dressing is gradually released together with HA from the wound dressing. Third important issue is to provide moist wound-healing environment. The upper layer and lower layer Sponge-Col can provide the wound dressing with high water absorption and long-term water retention properties.

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“…The sheet-shaped Col sponge was manufactured by the method described in our previous paper [17] [18]. The method was briefly described below.…”

Section: Manufacture Of Col Spongementioning

confidence: 99%

“…aqueous solution of Col and Col'[17] [18]. The Col aqueous solution (pH 2.8) precipitates when the pH value reaches 4 under 25˚C conditions.…”

mentioning

confidence: 99%

Design of Novel Wound Dressing Composed of Collagen and Hyaluronic Acid Containing Epidermal Growth Factor

Kuroyanagi¹,

Suzuki²,

Kuroyanagi³

2023

OJRM

Self Cite

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show abstract

“…Up to now, various collagen-based hemostatic materials have been studied, such as plastic, reconstructive, and oral surgery. [1][2][3] Collagen that is derived from mammalian sources may potentially cause infectious diseases. [4][5][6] Otherwise, collagen derived from fish scales can eliminate this risk.…”

Section: Introductionmentioning

confidence: 99%

Characterizations and bioactivities of novel biocomposites based on modified fish collagen, ginsenoside Rb1, and Camellia chrysantha polyphenols

Nguyen,

Duong,

Hoang

et al. 2024

J of Applied Polymer Sci

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Collagen derived from freshwater fish scales is a potential material for hemostatic and wound healing. 3D printing method is an environmentally friendly technique to geometrically‐controlled samples and is widely applied to prepare collagen‐based materials. This work aims to compare the advantages of preparation methods for hemostatic materials based on collagen, in which, the traditional method, solution will be compared with the modern method, 3D printing. Collagen in gel form was crosslinked with glutaraldehyde and was first time modified with ginsenoside Rb1 and Camellia chrysantha polyphenols. The characteristics of biomaterial samples, including functional groups, morphology, and element composition of samples have been assessed using infrared spectroscopy, energy‐dispersive x‐ray, field emission scanning electron microscopy. The swelling degree and hemostatic ability of biomaterial samples prepared according to two methods would be tested. The antibacterial, anti‐inflammatory tests, and in vivo test on mice were carried out on these biomaterials. The findings confirmed that the 3D printing method with direct ink writing technique is more suitable for the preparation of hemostatic collagen‐based membrane than the solution method. The time to hemostasis when using collagen‐based membrane was found to be 104.0 ± 16.7 s. Moreover, the collagen‐based biomaterials also have anti‐inflammatory and antibacterial activity. This opens potential applications of the biomaterials from fish scale collagen in practice.

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Design of Collagen-Based Hemostatic Material for Use in Plastic and Reconstructive Surgery

Cited by 2 publications

References 13 publications

Design of Novel Wound Dressing Composed of Collagen and Hyaluronic Acid Containing Epidermal Growth Factor

Design of Novel Wound Dressing Composed of Collagen and Hyaluronic Acid Containing Epidermal Growth Factor

Characterizations and bioactivities of novel biocomposites based on modified fish collagen, ginsenoside Rb1, and Camellia chrysantha polyphenols

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