Design of Chromatographic and Electrophoretic Separation Based on Ion-Pair Formation of Aromatic Disulfonates with Polyammonium Ions

Okada, Tetsuo

doi:10.1021/ac951096w

Cited by 14 publications

(7 citation statements)

References 17 publications

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“…Some multivalent cations have been used as pairing ions in liquid chromatography for the separation of NDS isomers with polyethyleneammonium ions 14 and inorganic anions with triethylenetetramine 15 and hexamethonium has been used for the separation of metallo-cyanide complexes in CZE. 16 However, the analysis of ion association reactions in aqueous solution have not previously been studied with such cations.…”

Section: Separation Of Positional Isomers With Divalent Ammonium Ionsmentioning

confidence: 99%

Separation of Divalent Aromatic Anions by Capillary Zone Electrophoresis Using Multipoint Ion Association With Divalent Quaternary Ammonium Ions

Takayanagi¹,

Wada²,

Motomizu³

1997

Analyst

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Positional isomers of naphthalenedisulfonate (NDS), naphthalenedicarboxylate (NDC) and phthalate were separated using an ion association reaction in aqueous solution followed by capillary zone electrophoresis with a polymer coated capillary. By using divalent quaternary ammonium ions, four NDS isomers and three NDC isomers were completely resolved, although some of the isomers were not resolved with tetrabutylammonium ion. Ion association constants, K ass , between the anions and the divalent quaternary ammonium cations were also determined by analyzing the change in electrophoretic mobility using a non-linear least-squares method. The K ass values of the anions obtained with the divalent cations were larger than those with tetrabutylammonium ion because of an increased electrostatic interaction. The ion associability was also explained on the basis of a distance-fitting concept and the hydrophobicity concept of the pairing cations. Ion associability was also improved by using a better distance-fitting pairing ion. From the results obtained, it was suggested that the charge-charge distance in cationic and anionic molecules must be close, and the charge-charge distance of the divalent cations was about 0.2 nm longer than that of divalent anions for good ion associability (a distance-fitting concept).

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Section: Separation Of Positional Isomers With Divalent Ammonium Ionsmentioning

confidence: 99%

Separation of Divalent Aromatic Anions by Capillary Zone Electrophoresis Using Multipoint Ion Association With Divalent Quaternary Ammonium Ions

Takayanagi¹,

Wada²,

Motomizu³

1997

Analyst

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“…Hexanesulfonic acid, under conditions where it is partly deprotonated, has been shown to enhance separation of polylysines of different lengths . Conversely, the separation of sulfonate analytes can be enhanced by addition of aliphatic compounds containing two or more ammonium groups . The selectivity was influenced by the match between the number and spacing of ammonium groups and analyte sulfonate groups.…”

Section: Introductionmentioning

confidence: 99%

Counterion condensation in short cationic peptides: Limiting mobilities beyond the Onsager–Fuoss theory

et al. 2012

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We investigated the effect of the background electrolyte (BGE) anions on the electrophoretic mobilities of the cationic amino acids arginine and lysine and the polycationic peptides tetraarginine, tetralysine, nonaarginine, and nonalysine. BGEs composed of sodium chloride, sodium propane-1,3-disulfonate, and sodium sulfate were used. For the amino acids, determination of the limiting mobility by extrapolation, using the Onsager-Fuoss (OF) theory expression, yielded consistent estimates. For the peptides, however, the estimates of the limiting mobilities were found to spuriously depend on the BGE salt. This paradox was resolved using molecular modeling. Simulations, on all-atom as well as coarse-grained levels, show that significant counterion condensation, an effect not accounted for in OF theory, occurs for the tetra- and nonapeptides, even for low BGE concentrations. Including this effect in the quantitative estimation of the BGE effect on mobility removed the discrepancy between the estimated limiting mobilities in different salts. The counterion condensation was found to be mainly due to electrostatic interactions, with specific ion effects playing a secondary role. Therefore, the conclusions are likely to be generalizable to other analytes with a similar density of charged groups and OF theory is expected to fail in a predictable way for such analytes.

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“…87 Divalent quaternary ammonium ions are also designed to utilize multipoint interactions. 88 The distances between two ammonium sites in the ion association reagents are controlled with the chain length of methylene group (Fig.…”

Section: ·3 Factors Contributing To Ion Association Reactions In Aqumentioning

confidence: 99%

Analysis of Ion-Association Reaction in Aqueous Solution and Its Utilization by Capillary Zone Electrophoresis

Takayanagi

2004

ANAL. SCI.

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Design of Chromatographic and Electrophoretic Separation Based on Ion-Pair Formation of Aromatic Disulfonates with Polyammonium Ions

Cited by 14 publications

References 17 publications

Separation of Divalent Aromatic Anions by Capillary Zone Electrophoresis Using Multipoint Ion Association With Divalent Quaternary Ammonium Ions

Separation of Divalent Aromatic Anions by Capillary Zone Electrophoresis Using Multipoint Ion Association With Divalent Quaternary Ammonium Ions

Counterion condensation in short cationic peptides: Limiting mobilities beyond the Onsager–Fuoss theory

Analysis of Ion-Association Reaction in Aqueous Solution and Its Utilization by Capillary Zone Electrophoresis

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