Design of antimicrobial polycaprolactam nanocomposite by immobilizing subtilisin conjugated Au/Ag core-shell nanoparticles for biomedical applications

Prabhawathi, Veluchamy; Sivakumar, Ponnurengam Malliappan; Thulasinathan, Boobalan; Manohar, Cynthya Maria; Doble, Mukesh

doi:10.1016/j.msec.2018.10.020

Cited by 33 publications

(11 citation statements)

References 64 publications

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“…The distribution and size of the NPs was more uniform for sample Au:Ag 1:9 as compared to 9:1. This result was in line with the previous report which demonstrated that Au-AgNPs formed spontaneously via self-organisation ( Zhang et al, 2013 ; Prabhawathi et al, 2019 ). Well-separated core-shell NPs were observed in Figure 1F .…”

Section: Resultssupporting

confidence: 93%

Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid delivery

Lee

Shameli

Nagao

et al. 2022

Front. Mol. Biosci.

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Colorectal cancer is one of the most killing cancers and this has become a global problem. Current treatment and anticancer drugs cannot specifically target the cancerous cells, thus causing toxicity towards surrounding non-cancer cells. Hence, there is an urgent need to discover a more target-specific therapeutic agent to overcome this problem. Core-shell nanoparticles have emerged as good candidate for anticancer treatment. This study aimed to synthesize core-shell nanoparticles via green method which utilised crude peels extract of Garcinia mangostana as reducing and stabilising agents for drug delivery. Gold-silver core-shell nanoparticles (Au-AgNPs) were synthesized through seed germination process in which gold nanoparticles acted as the seed. A complete coating was observed through transmission electron microscopy (TEM) when the ratio of AuNPs and AgNPs was 1:9. The size of Au-AgNPs was 38.22 ± 8.41 nm and was mostly spherical in shape. Plant-based drug, protocatechuic acid (PCA) was loaded on the Au-AgNPs to investigate their anticancer activity. In HCT116 colon cancer cells, PCA-loaded Au-AgNPs (IC50 = 10.78 μg/ml) showed higher inhibitory action than the free PCA (IC50= 148.09 μg/ml) and Au-AgNPs alone (IC50= 24.36 μg/ml). Up to 80% inhibition of HCT116 cells was observed after the treatment of PCA-loaded Au-AgNPs at 15.63 μg/ml. The PCA-loaded Au-AgNPs also showed a better selectivity towards HCT116 compared to CCD112 colon normal cells when tested at the same concentrations. These findings suggest that Au-AgNPs system can be used as a potent nanocarrier to combat cancerous cells by offering additional anticancer properties to the loaded drug.

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Section: Resultssupporting

confidence: 93%

Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid delivery

Lee

Shameli

Nagao

et al. 2022

Front. Mol. Biosci.

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“…[24][25][26] For instance, Ramyaa et al reported that dexamethasone-silver nanoparticles embedded in dendritic collagen matrix nanoparticles reduced biofilm formation in Staphylococcus aureus and Klebsiella pneumonia. 26 With the rapid development of nanotechnology, a lot of novel antimicrobial agents and methods have been put forward and studied, such as antibacterial peptides (AMP), 27,28 quaternary ammonium compounds, 29 quantum dots, 30 photodynamic therapy 31 and photothermal therapy. 32 Antimicrobial peptides are a diverse group of naturally occurring molecules, which can be produced by various kinds of living organisms including bacteria and animals.…”

Section: Introductionmentioning

confidence: 99%

“…With the rapid development of nanotechnology, a lot of novel antimicrobial agents and methods have been put forward and studied, such as antibacterial peptides (AMP), 27 , 28 quaternary ammonium compounds, 29 quantum dots, 30 photodynamic therapy 31 and photothermal therapy. 32 Antimicrobial peptides are a diverse group of naturally occurring molecules, which can be produced by various kinds of living organisms including bacteria and animals.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles

Xu¹,

Li²,

Wang³

et al. 2021

IJN

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Background:The biofilms could protect bacteria from antibiotics and promote the production of drug-resistant strains, making the bacteria more difficult to be eradicated. Thus, we developed an AMP@PDA@AgNPs nanocomposite, which is formed by modifying silver nanoparticles (AgNPs) with antimicrobial peptides (AMP) modified nanocomposite to destroy biofilm in this study. Methods: The AMP@PDA@AgNPs nanocomposite was prepared with polymerization method and characterized by using ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), Fourier transform-infrared spectroscopy (FT-IR), and transmission electron microscope (TEM). The antibacterial effects of the nanocomposite were investigated by using agar diffusion method and minimum inhibitory concentration (MIC) test. The quantitative analysis of the biofilm formation by the nanocomposite was conducted using crystal violet staining and confocal laser scanning microscope (CLSM). Results: The DLS and TEM analysis showed it was a spherical nanocomposite with 200 nm size and well dispersed . The results of UV-vis and FT-IR confirmed the presence of AMP and AgNPs. The nanocomposite had an excellent biocompatibility at 100 μg/mL. And the AMP@PDA@AgNPs nanocomposite showed superior antimicrobial activity against both Gram-negative (E. coli, P. aeruginosa) and Gram-positive (S. aureus) bacteria than AgNPs or AMP. Importantly, the mRNA expression of biofilm-related genes were decreased under the action of the nanocomposites. Conclusion: An AMP@PDA@AgNPs nanocomposite with good biocompatibility was successfully prepared. The nanocomposite could destruct bacterial biofilms by inhibiting the expression of biofilm-related genes. The synergistic strategy of AMPs and AgNPs could provide a new perspective for the treatment of bacterial infection.

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“…One milliliter of [Ac] F @Fe 3 O 4 –PG-treated solution was taken after centrifugation at 5000 rpm and refrigerated to 10 °C immediately. The concentration of protein was analyzed using Lowry’s method by measuring the optical density at 660 nm. , Nucleic acid content and its derivative compounds such as purines and pyrimidine were measured using a UV–visible spectrophotometer at 260 nm, as described by Nguyen et al All of the above experiments were investigated in triplicate, and the average values were reported.…”

Section: Methodsmentioning

confidence: 99%

Prodigiosin–Iron-Oxide–Carbon Matrix for Efficient Antibiotic-Resistant Bacterial Disinfection of Contaminated Water

Arivizhivendhan

Mahesh

Murali

et al. 2019

ACS Sustainable Chem. Eng.

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Cheap and efficient bioactive materials capable of producing potable water are in high demand. We synthesized bioactive prodigiosin-conjugated iron-oxide-activated carbon composite ([Ac] F @Fe 3 O 4 −PG) for the effective removal of microbial contaminants without biofilm formation. The disinfection effect of [Ac] F @Fe 3 O 4 −PG was investigated against antibiotic-resistant Escherichia coli and Bacillus cereus. The reactive oxygen species, glutathione concentration, and intracellular leakages assay, zeta potential, atomic force microscopy, scanning electron microscopy, and fluorescence microscopy were performed to understand the mechanistic route of bacterial disinfection. Results demonstrate the bacterial cell membrane damage due to the surface charge neutralization by cationic [Ac] F @Fe 3 O 4 −PG, thereby affecting the transport system of the cells. The generation of reactive oxygen species further triggered programmed bacterial cell death with apoptosis-like changes in cellular morphology. We demonstrate that the [Ac] F @Fe 3 O 4 −PG matrix is reusable and has long-term antibacterial activity with efficient inhibition of biofilm formation. The study reveals that the [Ac] F @Fe 3 O 4 −PG could be a promising disinfectant for both Gram-negative and Gram-positive pathogens for effective environmental applications.

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Design of antimicrobial polycaprolactam nanocomposite by immobilizing subtilisin conjugated Au/Ag core-shell nanoparticles for biomedical applications

Cited by 33 publications

References 64 publications

Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid delivery

Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid delivery

Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles

Prodigiosin–Iron-Oxide–Carbon Matrix for Efficient Antibiotic-Resistant Bacterial Disinfection of Contaminated Water

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