Design of antimicrobial and cytolytic peptides by computational analysis of bacterial, algal, and invertebrate proteomes

Duque-Salazar, Geraldine; Mendez-Otalvaro, Edward; Ceballos-Arroyo, Alberto Mario; Ordúz, Sergio

doi:10.1007/s00726-020-02900-w

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…AMPs are usually hydrophobic and this provides the peptides with the ability to permeate cell membranes, which are constituted by phospholipid bilayers [ 18 ]. The hydrophobic residues can bind with the inner core of the cellular plasma membrane, and the higher hydrophobicity causes the insertion of the peptides into the membrane [ 19 ] such that the peptides have the potential to disrupt the mammalian cell membrane, which leads to their toxicity towards normal cells [ 20 ]. Based on previous studies, there are several strategies that can be used reduce cytotoxicity against normal cells.…”

Section: Introductionmentioning

confidence: 99%

Discovery of a Novel Antimicrobial Peptide, Temporin-PKE, from the Skin Secretion of Pelophylax kl. esculentus, and Evaluation of Its Structure-Activity Relationships

et al. 2022

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Bacterial resistance against antibiotics has led to increasing numbers of treatment failures, and AMPs are widely accepted as becoming potential alternatives due to their advantages. Temporin-PKE is a novel peptide extracted from the skin secretion of Pelophylax kl. esculentus and it displays a strong activity against Gram-positive bacteria, with an extreme cytotoxicity. Incorporating positively charged residues and introducing D-amino acids were the two main strategies adopted for the modifications. The transformation of the chirality of Ile could reduce haemolytic activity, and an analogue with appropriate D-isoforms could maintain antimicrobial activity and stability. The substitution of hydrophobic residues could bring about more potent and broad-spectrum antimicrobial activities. The analogues with Lys were less harmful to the normal cells and their stabilities remained at similarly high levels compared to temporin-PKE. The optimal number of charges was three, and the replacement on the polar face was a better choice. Temporin-PKE-3K exerted dually efficient functions includingstrong antimicrobial and anticancer activity. This analogue showed a reduced possibility for inducing resistance in MRSA and Klebsiella pneumoniae, a rather strong antimicrobial activity in vivo, and it exhibited the highest therapeutic index such that temporin-PKE-3K has the potential to be developed as a clinical drug.

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Section: Introductionmentioning

confidence: 99%

Discovery of a Novel Antimicrobial Peptide, Temporin-PKE, from the Skin Secretion of Pelophylax kl. esculentus, and Evaluation of Its Structure-Activity Relationships

et al. 2022

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Humanizing plant-derived snakins and their encrypted antimicrobial peptides

2022

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Antimicrobial Peptides from Photosynthetic Marine Organisms with Potential Application in Aquaculture

2023

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Aquaculture production is at a record level and is estimated to increase in the coming years. However, this production can be negatively affected by infectious diseases produced by viruses, bacteria, and parasites, causing fish mortality and economic losses. Antimicrobial peptides (AMPs) are small peptides that may be promising candidates to replace antibiotics because they are the first line of defense in animals against a wide variety of pathogens and have no negative effects; they also show additional activities such as antioxidant or immunoregulatory functions, which makes them powerful alternatives for use in aquaculture. Moreover, AMPs are highly available in natural sources and have already been used in the livestock farming and food industries. Photosynthetic marine organisms can survive under all kinds of environmental conditions and under extremely competitive environments thanks to their flexible metabolism. For this reason, these organisms represent a powerful source of bioactive molecules as nutraceuticals and pharmaceuticals, including AMPs. Therefore, in this study we reviewed the present knowledge about AMPs from photosynthetic marine organism sources and analyzed whether they could be suitable for use in aquaculture.

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Design of antimicrobial and cytolytic peptides by computational analysis of bacterial, algal, and invertebrate proteomes

Cited by 3 publications

References 25 publications

Discovery of a Novel Antimicrobial Peptide, Temporin-PKE, from the Skin Secretion of Pelophylax kl. esculentus, and Evaluation of Its Structure-Activity Relationships

Discovery of a Novel Antimicrobial Peptide, Temporin-PKE, from the Skin Secretion of Pelophylax kl. esculentus, and Evaluation of Its Structure-Activity Relationships

Humanizing plant-derived snakins and their encrypted antimicrobial peptides

Antimicrobial Peptides from Photosynthetic Marine Organisms with Potential Application in Aquaculture

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