The measurement of chemical, physical and biological parameters is important for the characterization of streams health. Thus, cost effective and targeted water quality (WQ) monitoring programmes are required for proper assessment, restoration and protection of such streams. This research proposes a WQ monitoring network for the Limpopo River Basin (LRB) in Mozambique located in Southern Africa, a region prone to severe droughts. In this Basin both anthropogenic and natural driven processes, exacerbated by the increase water demand by the four riparian countries (Botswana, South Africa, Zimbabwe and Mozambique) are responsible for the degradation of surface waters, impairing their downstream use either for aquatic ecosystem, drinking, industrial or irrigation. Hence, physic-chemical, biological and microbiological characteristics at 23 sites within the basin were studied in November-2006 and January-2007. The assessment of the final WQ condition at sampled points was done taking into account the Mozambican guidelines for receiving waters and the environmental WQ standards for effluent discharges together with the WHO guidelines for drinking WQ. The assessed data indicated that sites located at proximities to the border with upstream countries were contaminated with heavy metals. The Elephants subcatchment was found with a relatively better WQ whereas the Changane subcatchment together with the effluent point discharges were found polluted as indicated by the low dissolved oxygen and high total dissolved solids, electric conductivity, total hardness, sodium adsorption ratio and low benthic macroinvertebrates taxa. Significant differences (p<0.05) were found for some parameters when the concentrations recorded in November and January were tested, therefore indicating possible need for monthly monitoring of WQ. From this study it was concluded that a systematic WQ monitoring network composed of 16 stations would fit the conditions of the LRB. Ambient, earl warning, operational and effluents are the main monitoring types recommended. Additional research at a Basin scale was * Corresponding author. Tel.: +258824449340; Fax: +288221492176 e-mail: mchilundo@uem.mz 2 also recommended to identify the major sources, transport and impacts to the downstream ecosystem.