Design of a synthetic adhesion protein by grafting RGD tailed cyclic peptides on bovine serum albumin

Abstract

SummaryA synthetic adhesion protein was designed by chemical grafting of the RGD tailed cyclic peptide cyclo[-D-Val-ArgGly-Asp-Glu(-EAhx-Tyr-Cys-NH~)-] on the carrier protein bovine serum albumin (BSA). The cyclic conformation of the RGD motif grafted on the protein mimics the conformation of the motif displayed in native adhesion proteins such as fibronectin. The adhesion of the cells on polystyrene coated with the conjugate BSA-peptide was similar or even better than the one obtained when the proadhesive protein fibronectin was coated on the plates. Results also indicated that covalent coupling of the peptide on BSA is not absolutely required, since simple adsorption of the peptide on the protein coated on plates was efficient for enhancing cell adhesion. These results show that polystyrene support can be reconditioned with conformationally constrained RGD peptides to enhance cell adhesion on solid supports. The same methodology can be adapted for the development of new biomaterials based on the recognition of specific peptides.

Immobilisation of linear and cyclic RGD-peptides on titanium surfaces and their impact on endothelial cell adhesion and proliferation

“…It is of much interest to examine the conformation of the RGD-peptide, which once immobilised to titanium surfaces, will be able to improve biointegration of an implant by stimulation of ECs. The cyclic peptide seems to enhance the binding (Delforge et al, 1998;Hsiong et al, 2008;Zhu et al, 2009). A difference in EC cell coverage and proliferation on titanium surfaces by means of the structure of RGD peptides, which could be shown in our study, has not been investigated.…”

Peptide‐modified bone repair materials: Factors influencing osteogenic activity

“…Specifically, it has been found that conformation of RGD‐containing peptides affect cell binding and can control the specificity to an integrin. In addition, cyclic‐RGD peptides immobilized on the material surfaces have a more effective promotion on cell attachment and a higher in vivo stability compared to the linear‐based peptides . It may benefit from the decreased structural flexibility exerted by the ring .…”

“…In addition, cyclic-RGD peptides immobilized on the material surfaces have a more effective promotion on cell attachment and a higher in vivo stability compared to the linear-based peptides. 19,[39][40][41] It may benefit from the decreased structural flexibility exerted by the ring. 42 Moreover, this rigidity stabilizes the Asp side chain carboxylic acid, which helps avoid proteases attack when orientating itself in a suitable position.…”

Conception, élaboration et caractérisation de matériaux bioactifs

“…En effet, van der Pluijm et al [50] et Delforge et al [51] ont montré une augmentation de l'attachement cellulaire en utilisant leurs peptides cycliques comparativement à des peptides linéaires. Par ailleurs, le peptide cyclique c(-RGDfX-) pour lequel l'acide aminé D suit la fonction aspartate induit une conformation de la séquence RGD telle que celle-ci est mieux reconnue par les intégrines av et présente une affinité très largement réduite vis-à-vis des récepteurs plaquettaires aIIbb3 [52,53].…”