Design of a Specific Colonic Mucus Marker Using a Human Commensal Bacterium Cell Surface Domain

Aims: To determine the relationship between adhesive ability of probiotics and acidic residues in human colonic mucin, we developed a new screening method using Biacore to evaluate adherence of bacteria before and after sialic acid or sulphate residues were blocked or removed from mucin. Methods and Results: Ten strains of lactobacilli and three strains of bifidobacteria isolated from human faeces were evaluated for their adhesive properties to soluble human colonic mucin (sHCM) using the Biacore binding assay. Three strains (Lactobacillus strain ME-522, Lact. gasseri ME-527 and Bifidobacterium bifidum MCC1092) showing significant adherence were selected. Decreased binding activities were observed after removing sialic acid of sHCM using sialidase. However, after removing the sulphate residue using sulphatase, the adhesion of ME-527 decreased; whereas the remaining two strains had increased adhesion. The adhesion of three probiotics significantly decreased after the sulphate residue was blocked by elution with barium chloride. Conclusions: A new evaluation method using the Biacore assay was developed to observe binding properties to the acidic residues of sHCM. Results indicated that there was a strong relationship between probiotic adhesion and acidic residues of sHCM. Significance and Impact of the Study: This is the first report showing a screening method that quantitatively measures the binding between bacteria and acidic residues in sHCM using the Biacore binding assay; and provides a new method for the selection of probiotics in the future.

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“…; Coic et al . ); and therefore there is a lack of clarity about interactions between probiotics and acidomucins.…”

Section: Discussionmentioning

confidence: 99%

New screening methods for probiotics with adhesion properties to sialic acid and sulphate residues in human colonic mucin using the Biacore assay

et al. 2013

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“…Here, the adherent bacteria could be considered as a crucial link with the environment, offering interactions with stem cells to yield focal lesions. In this regard the mucus layer [Coic et al 2012] and crypt-specific core microbiota [Pédron et al 2012] are considered as facilitators. In the two main hereditary syndromes involving no lethal germline mutations (APC, mismatch repair system) the delay for achieving critical accumulation of mutations in the stem cells up to the development of cancer is significantly shorter (20-45 years age) than in sporadic cancers (65 years age).…”

Section: Stem Cell As Pivotal Target From Normal To Neoplastic Lesionmentioning

confidence: 99%

Microbial dysbiosis and colon carcinogenesis: could colon cancer be considered a bacteria-related disease?

Sobhani

Amiot

Baleur

et al. 2013

Therap Adv Gastroenterol

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124

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Colorectal cancer (CRC) is posing an increasingly important burden on the health care system, with western countries seeing a growing incidence of the disease. Except for germline DNA mutations which have been attributed to less than 5% of patients, little is known about the main causes of CRC. However, environment factors such as food, lifestyle and medication are now suspected to have a major influence on inducing cancers. Today, exhaustive quantitative and qualitative evaluation of all environmental factors is not possible. Various environment-induced diseases have been characterized based on colon microflora, also called microbiota, analyses. Growing data have shown specific changes in microflora (i.e. dysbiosis) in the stools of patients with colon cancer or those adherent to the colonic mucosa. Thus, it appears that microbiota may be considered a platform offering host and environment interactions for studying CRCs. The hypothesis that colon cancer might be a bacteria-related disease is suggested and perspectives are discussed.

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“…Mub repeats and MucBP domains have been shown to bind to mucins and Igs in vitro (Roos and Jonsson, 2002;Pretzer et al, 2005;Bumbaca et al, 2007;MacKenzie et al, 2009;von Ossowski et al, 2011;Coic et al, 2012;Watanabe et al, 2012). To gain insights into the binding of full-length MUB to mucus, adhesion assays were carried out using a human mucus-secreting intestinal cell line and mammalian tissue sections.…”

Section: Reuteri Native Mub Binds To Mucus Via Terminal Sialylatedmentioning

confidence: 99%

“…The B1 domain displays structural and functional similarity to Protein L from Peptostreptococcus magnus (PpL), a Fabdependent immunoglobulin-binding protein (Graille et al, 2001), and the B2 domain corresponds to MucBP (mucinbinding protein) in the Pfam database (PF06458). Mub repeats and MucBP domains have been shown to bind to mucins and glycans (Roos and Jonsson, 2002;Pretzer et al, 2005;Bumbaca et al, 2007;von Ossowski et al, 2011;Coic et al, 2012;Watanabe et al, 2012). Numerous MUB homologues and MucBP domain-containing proteins have been found almost exclusively in lactic acid bacteria and predominantly in lactobacilli naturally located in intestinal niches (Boekhorst et al, 2006;Kleerebezem et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Structural basis for adaptation of lactobacilli to gastrointestinal mucus

Etzold

Kober

MacKenzie

et al. 2014

Environmental Microbiology

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SummaryThe mucus layer covering the gastrointestinal (GI) epithelium is critical in selecting and maintaining homeostatic interactions with our gut bacteria. However, the underpinning mechanisms of these interactions are not understood. Here, we provide structural and functional insights into the canonical mucus-binding protein (MUB), a multi-repeat cellsurface adhesin found in Lactobacillus inhabitants of the GI tract. X-ray crystallography together with small-angle X-ray scattering demonstrated a 'beads on a string' arrangement of repeats, generating 174 nm long protein fibrils, as shown by atomic force microscopy. Each repeat consists of tandemly arranged Ig-and mucin-binding protein (MucBP) modules. The binding of full-length MUB was confined to mucus via multiple interactions involving terminal sialylated mucin glycans. While individual MUB domains showed structural similarity to fimbrial proteins from Gram-positive pathogens, the particular organization of MUB provides a structural explanation for the mechanisms in which lactobacilli have adapted to their host niche by maximizing interactions with the mucus receptors, potentiating the retention of bacteria within the mucus layer. Together, this study reveals functional and structural features which may affect tropism of microbes across mucus and along the GI tract, providing unique insights into the mechanisms adopted by commensals and probiotics to adapt to the mucosal environment.

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Design of a Specific Colonic Mucus Marker Using a Human Commensal Bacterium Cell Surface Domain

Cited by 16 publications

References 30 publications

New screening methods for probiotics with adhesion properties to sialic acid and sulphate residues in human colonic mucin using the Biacore assay

New screening methods for probiotics with adhesion properties to sialic acid and sulphate residues in human colonic mucin using the Biacore assay

Microbial dysbiosis and colon carcinogenesis: could colon cancer be considered a bacteria-related disease?

Structural basis for adaptation of lactobacilli to gastrointestinal mucus

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