Design of a novel optical sensor for determination of trace amounts of copper by UV–visible spectrophotometry in real samples

Abstract

A new optical chemical sensor is established for sensitive and selective spectrophotometric detection of copper based on the immobilization of 3‐(2‐methyl‐2,3‐dihydro‐1,3‐benzothiezol‐2‐yl)‐2H‐chromen‐2‐one on a triacetylcellulose membrane. Copper ions react with the immobilized ligand and cause an increase in the absorption of the membrane at 550 nm in universal buffer solution at pH = 6. The effects of pH, indicator concentration and reaction time on the immobilization of the ligand were studied. This optode exhibits a linear range of 7.0 × 10−7 to 1 × 10−4 mol l−1 of copper ion concentration with a limit of detection of 3.0 × 10−7 mol l−1. The response time of the newly designed optode is within 3 min. The effect of different possible interfering species was investigated and it was found that the sensor has very good selectivity. The proposed sensor benefits from advantages such as low cost, high stability, low detection limit, fast response time, reproducibility, relatively long lifetime, and good selectivity for Cu2+ ion determination among a large number of alkali, alkaline earth, transition and heavy metal ions. The sensor can readily be regenerated with thiourea solution and its response is reversible and reproducible (relative standard deviation < 1.4%). The proposed optode was applied successfully for the determination of Cu(II) in various samples.

Biological Activity and Optical Sensor Properties of Green Synthesis Polymer

“…In this study, 4-amino-3-hydroxy-1-naphthalenesulfonic acid monomer was polymerized with Horseradish peroxidase (HRP) enzyme. Structural characterization of this water-soluble polymer was assigned by FT-IR, 1 H-NMR and 13 C-NMR measurements. In FT-IR, a C-NH peak at 1384 cm-1 and a CÀ O-C peak at 1060 cm À 1 belonging to the polymerization of amine and hydroxyl groups were observed.…”

“…Carbon signals of the sulphonic acid group at 183.05 ppm, the hydroxyl group at 153.53 ppm, the amine group at 131.46 ppm, and the aromatic ring in the range of 133.93-106.97 ppm were seen. In the 13 C-NMR spectrum of the poly-enz, the ipso carbon of the OH group was observed at 150.28 ppm, and the À CÀ OÀ CÀ peak of the enzymatic polymerization was observed at 158.75 ppm. The ipso carbon of the NH2 group was assigned at 132.02 ppm, and the À CÀ NÀ CÀ peak of the bonding at 143.42 ppm.…”

“…The 1 H-NMR and 13 C-NMR spectra of the monomer and the synthesized Poly-enz were taken in deuterium oxide (D 2 O). The clear structure of the monomer and enzymatic polymer, the labelling of the protons and the chemical shifts of all protons are given in (Figure 2) and (Figure 3).…”

“…In these it is used to monitor analyzes of heavy metal ions. [10][11][12][13][14] Considering this, polymer-metal complex was studied in UV-Vis spectrophotometer. Enzymatic and chemical oxidative polymers were synthesized in Horseradish peroxidase (HRP)/hydrogen peroxide (H 2 O 2 ), 4-aminosalicylic acid, [15] carbazole containing hydroxy functional group, [16] 2,6-diamino pyridine.…”

“…An ion sensitive optical fiber device is obtained from metals with optical sensor properties. In these it is used to monitor analyzes of heavy metal ions [10–14] . Considering this, polymer‐metal complex was studied in UV‐Vis spectrophotometer.…”