Design of a multi‐epitope‐based vaccine consisted of immunodominant epitopes of structural proteins of SARS‐CoV‐2 using immunoinformatics approach

Mahmoudvand, Shahab; Ghaleh, Hadi Esmaeili Gouvarchin; Jalilian, Farid Azizi; Farzanehpour, Mahdieh; Dorostkar, Ruhollah

doi:10.1002/bab.2431

Cited by 2 publications

(2 citation statements)

References 70 publications

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“…The study utilized EAAAK, GGGS, and HEYGAEALERAG linkers to establish connections between various vaccine components. These linkers are crucial in determining both B and T cells’ 3D structure and subsequent exposure [ 43 , 44 ]. The “EAAAK” linker attached the Adjuvant to vaccination sequences.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have shown the beneficial use of RV, highlighting the need for vaccination as a preventive and cost-effective strategy against this virus. Researchers used reverse vaccinology and subtractive proteomics techniques in this study to ascertain prospective therapeutic interventions for A. baumannii [ 11 , 16 , 43 ]. The recombinant chimeric peptide vaccine demonstrated a 50% efficacy in hamsters against the pathogen during recent in vitro testing [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

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A novel chimeric vaccine containing multiple epitopes for simulating robust immune activation against Klebsiella pneumoniae

Hakimian,

Doosti,

Sharifzadeh

2024

BMC Immunol

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Background Due to antibiotic resistance, the Klebsiella genus is linked to morbidity and death, necessitating the development of a universally protective vaccine against Klebsiella pathogens. Methods Core sequence analysis prioritized non-redundant host molecules and expected lipid bilayer peptides from fully sequenced Klebsiella genomes. These proteins were refined to identify epitopes, examining their immunogenicity, toxicity, solubility, and interaction with MHC alleles. Epitopes were linked to CPG ODN C274 via EAAAK, HEYGAEALERAG, and GGGS linkers to enhance immunological responses. The vaccine’s tertiary structure was modelled and docked with MHC-I and MHC-II. Results Fifty-five proteins were recognized in the Vaxign collection as having remarkable features. Twenty-three proteins with potential pathogenicity were then identified. Eight options for vaccines emerged after the immunogenicity of proteins was examined. The best antigens were three proteins: MrkD, Iron-regulated lipid membrane polypeptides, and RmpA. These compounds were selected for their sensitivity. The structural protein sequences of K. pneumoniae were utilized to identify seven CTL epitopes, seven HTL epitopes, and seven LBL epitopes, respectively. The produced immunization displayed a stable contact with the receptors, based on molecular dynamic simulations lasting 250 nanoseconds. Intermolecular binding free energies also indicated the dominance of the van der Waals and electrostatic energies. Conclusion In summary, the results of this study might help scientists develop a novel vaccine to prevent K. pneumoniae infections.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel chimeric vaccine containing multiple epitopes for simulating robust immune activation against Klebsiella pneumoniae

Hakimian,

Doosti,

Sharifzadeh

2024

BMC Immunol

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Design of multivalent-epitope vaccine models directed toward the world’s population against HIV-Gag polyprotein: Reverse vaccinology and immunoinformatics

Hashempour,

Khodadad,

Bemani

et al. 2024

PLoS ONE

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Significant progress has been made in HIV-1 research; however, researchers have not yet achieved the objective of eradicating HIV-1 infection. Accordingly, in this study, eucaryotic and procaryotic in silico vaccines were developed for HIV-Gag polyproteins from 100 major HIV subtypes and CRFs using immunoinformatic techniques to simulate immune responses in mice and humans. The epitopes located in the conserved domains of the Gag polyprotein were evaluated for allergenicity, antigenicity, immunogenicity, toxicity, homology, topology, and IFN-γ induction. Adjuvants, linkers, CTLs, HTLs, and BCL epitopes were incorporated into the vaccine models. Strong binding affinities were detected between HLA/MHC alleles, TLR-2, TLR-3, TLR-4, TLR-7, and TLR-9, and vaccine models. Immunological simulation showed that innate and adaptive immune cells elicited active and consistent responses. The human vaccine model was matched with approximately 93.91% of the human population. The strong binding of the vaccine to MHC/HLA and TLR molecules was confirmed through molecular dynamic stimulation. Codon optimization ensured the successful translation of the designed constructs into human cells and E. coli hosts. We believe that the HIV-1 Gag vaccine formulated in our research can reduce the challenges faced in developing an HIV-1 vaccine. Nevertheless, experimental verification is necessary to confirm the effectiveness of these vaccines in these models.

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Design of a multi‐epitope‐based vaccine consisted of immunodominant epitopes of structural proteins of SARS‐CoV‐2 using immunoinformatics approach

Cited by 2 publications

References 70 publications

A novel chimeric vaccine containing multiple epitopes for simulating robust immune activation against Klebsiella pneumoniae

A novel chimeric vaccine containing multiple epitopes for simulating robust immune activation against Klebsiella pneumoniae

Design of multivalent-epitope vaccine models directed toward the world’s population against HIV-Gag polyprotein: Reverse vaccinology and immunoinformatics

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