Design of a microfabricated device for ligase detection reaction (LDR)

Abstract

would be possible. I would like to thank my major professor Dr. Michael Murphy, who offered me this extraordinary opportunity to do research in the Bio-MEMS group. His vision, knowledge, experience and encouragement was vital for me to go through and finish the work. I also would like to thank Dr. Steven Soper from the Chemistry Department and Dr. Dimitri Nikitopoulos from the Mechanical Engineering, without their input and knowledge this work would not have been possible. I would like to show my appreciation to fellow students Amit Maha, for significant contribution towards the mixer development, Dr. Yun Wang, Hashimoto, Daniel Pin-Chen, Michael Mitchell who helped with experiments and made insightful suggestions. To everybody in the Muset Lab, thank you for providing a good work atmosphere that enabled a person to strive not only academically but also socially. I thank the entire staff at CAMD for given the privilege to use the facility during the fabrication of my project. I would like to express my gratitude to Chenelle, family and friends who have always been a source of strength and encouragement.

Thermal Analysis of a Micro Device Used for Detection of Colorectal Cancer

“…The 95°C to 65°C temperature cycle would be repeated 20 times, at which time the reaction is stopped by cooling to 0°C. All of the temperature regions had to be isolated and insulated from each other and the other sections of the chip, such as the mixing stages and the identification section, in order to ensure that no thermal effects occurred in those regions [10]. Hence, simulation of the thermal response of any LDR device, which should be used for detection of cancer-associated rare gene mutations, is a major step that should be done during the device design and/or operation.…”

“…The medical device design life cycle as defined by the FDA[8] Layout of the LDR device[10] Temperature distribution and profile in the different thermal zones of the device for 0.5, 1, 1.5 and 2 mm size of air slits Temperature distribution and profile in the different thermal zones of the device for 0.5, 1, 1.5 and 2 mm size of air slits with side cuts…”