Design of a MEMS Tunable Polymer Grating for Single Detector Spectroscopy

Truxal, Steven C.; Kurabayashi, Katsuo; Tung, Yi-Chung

doi:10.1080/15599610802081779

Cited by 28 publications

(16 citation statements)

References 17 publications

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“…As a result, reliable and repeatable data acquisition is achieved. With the grating periodicity known for any actuation voltage level, the wavelength of the monochromatic light incident at the angle of the PMT is calculated using the grating equation, which allows us to construct spectrum plots 12,21. In this measurement, nearly five spectral sweeps within a 5 ms time window can be performed for each passage of a single microsphere in front of the optical fiber probe (Figure 2C).…”

Section: Resultsmentioning

confidence: 99%

Multiplexed Spectral Signature Detection for Microfluidic Color-Coded Bioparticle Flow

et al. 2010

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Here, we report a high-speed photospectral detection technique capable of discriminating subtle variations of spectral signature among fluorescently labeled cells and microspheres flowing in a microfluidic channel. The key component used in our study is a strain-tunable nanoimprinted grating microdevice coupled with a photomultiplier tube (PMT). The microdevice permits acquisition of the continuous spectral profiles of multiple fluorescent emission sources at 1 kHz. Optically connected to a microfluidic flow chamber via a multimode optical fiber, our multiwavelength detection platform allows for cytometric measurement of cell groups emitting nearly identical fluorescence signals with a maximum emission wavelength difference as small as 5 nm. The same platform also allows us to demonstrate microfluidic flow cytometry of four different microsphere types in a wavelength bandwidth as narrow as 40 nm at a high (>85%) confidence level. Our study shows that detection of fluorescent spectral signatures at high speed and high resolution can expand specificity of multicolor flow cytometry. The enhanced capability enables multiplexed analysis of color-coded bioparticles based on single-laser excitation and single-detector spectroscopy in a microfluidic setting. The fluorescence signal discrimination power achieved by the optofluidic technology holds great promise to enable quantification of cellular parameters with higher accuracy as well as enumeration of a larger number of cell types than conventional flow cytometric methods.Fluorescently color-coded cells, biomolecules, and microspheres enable multiparameter detection in disease screening, 1 molecular imaging, 2 DNA sequencing, 3 microarray applications, 4,5 cellular function studies, 6 and multiplexed bead-based assays. 7 The ability to identify multiple fluorescence emissions with high specificity is needed to gain sufficient multiplicity in biological analytical methods. Color decoding achieved by analyzing the detailed spectral characteristics of fluorophores can meet this demand, allowing fluorescence emissions with significant spectral overlap to be distinguished. 6, 8 -10 However, detection of multiple spectral signatures generally reduces measurement speed and throughput due to a larger volume of required information and suffers from the added complexity and cost of © 2010 American Chemical Society * To whom correspondence should be addressed. katsuo@umich.edu. SUPPORTING INFORMATION AVAILABLEAdditional information as noted in text. This material is available free of charge via the Internet at http://pubs.acs.org. Here, selective identification of multiple fluorescent molecular markers is also essential to discriminate diverse analyte species in the original sample mixture. However, a large hurdle against achieving high specificity and accuracy in multicolor flow cytometry arises when there are similarities and overlaps in the emission spectra of different dyes.14 For example, flow cytometers using as many as 11 fluorescent probe colors have been demonst...

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Section: Resultsmentioning

confidence: 99%

Multiplexed Spectral Signature Detection for Microfluidic Color-Coded Bioparticle Flow

et al. 2010

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“…According to equation (5), the relation between spectral width of quasi-monochromatic light source and the difference of FWHM (ΔF) is defined by…”

Section: Sensing Properties Of Modulating the Interdigitated Depthmentioning

confidence: 99%

“…One of horizontal modulated gratings is the accordion grating, which can be stretched perpendicular to the beams (in the plane of the grating). This changes the period of the grating and hence the wavelength diffracted at a particular angle or the [4] and miniature spectrometer [5]. In this paper, we propose a new horizontal modulated grating which is based on interdigitated comb structures.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of sensors based on MEMS grating with interdigitated comb structures

Wei

Wang

Yao

et al. 2010

5th International Symposium on Advanced Optical Manufacturing and Testing Technologies: Design, Manufacturing, and Testing of M

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Gratings as important spectral components have been employed in various optics applications, such as spectral analysis, filtering, dispersion compensation, sensing and so on. However, the physical structure of gratings produced by conventional technologies can not be alterable, this limits their applications under some specific requirements.Fortunately, MEMS technology breaks through that restriction, an interdigitated comb structure has been demonstrated in this paper. The comb structure has two sets of comb gratings; one is stationary and the other is movable in the horizontal plane. By driving the movable comb gratings, the intensity of diffraction will be adjustable. Under the condition of Fraunhofer approximation, the broadening extent of zero-order diffraction is monotonically increasing with the longitudinal displacement, and the relation between the intensity of first-order diffraction and the lateral displacement is a cosine squared function. A displacement sensor based on movable comb structures is presented and detailed analysis on sensitivity factors is given.

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“…Previous work in the area of strain-tunable optical devices, which have exploited far-field phenomena, include strain-tunable filters, 13 reflectors, 14 and gratings, 15 and dynamic color tuning. 16 Work involving near-field phenomena has included strain-tunable surface plasmon resonance, 17,18,19,20 fano resonance, 21 and controlling SERS activity .…”

Section: Introductionmentioning

confidence: 99%

SERS-enhanced piezoplasmonic graphene composite for biological and structural strain mapping

et al. 2017

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Thin-film optical strain sensors have the ability to map small deformations with spatial and temporal resolution and do not require electrical interrogation. This paper describes the use of graphene decorated with metallic nanoislands for sensing of tensile deformations of less than 0.04% with a resolution of less than 0.002%. The nanoisland-graphene composite films contain gaps between the nanoislands, which when functionalized with benzenethiolate behave as hot spots for surface-enhanced Raman scattering (SERS). Mechanical strain increases the sizes of the gaps; this increase attenuates the electric field, and thus attenuates the SERS signal. This compounded, SERS-enhanced “piezoplasmonic” effect can be quantified using a plasmonic gauge factor, and is among the most sensitive mechanical sensors of any type. Since the graphene-nanoisland films are both conductive and optically active, they permit simultaneous electrical stimulation of myoblast cells and optical detection of the strains produced by the cellular contractions.

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Design of a MEMS Tunable Polymer Grating for Single Detector Spectroscopy

Cited by 28 publications

References 17 publications

Multiplexed Spectral Signature Detection for Microfluidic Color-Coded Bioparticle Flow

Multiplexed Spectral Signature Detection for Microfluidic Color-Coded Bioparticle Flow

Characteristics of sensors based on MEMS grating with interdigitated comb structures

SERS-enhanced piezoplasmonic graphene composite for biological and structural strain mapping

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