Design of a Conformation‐Sensitive Xenon‐Binding Cavity in the Ribose‐Binding Protein

Lowery, Thomas J.; Rubin, Seth M.; Ruiz, Eliseo; Pines, Alexander; Wemmer, David E.

doi:10.1002/ange.200460629

Cited by 10 publications

(16 citation statements)

References 20 publications

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“…Xenon binding to myoglobin has been well characterized by NMR spectroscopy (43,44) and X-ray crystallography (45), and metmyoglobin-Xe association constants have been measured to be approximately 200 and 10 M −1 (45,46). Comparable affinities have been determined for other naturally occurring sites in hemoglobin (44), lipoxygenase (47), and lipid transfer protein (48), as well as specially designed hydrophobic cavities in T4 lysozyme (49), ribose-binding protein (50), and other examples (51,52). We showed previously that xenon binds water-soluble cryptophanes approximately 1.5-fold less avidly in human plasma than in aqueous buffer solution (14).…”

Section: Resultsmentioning

confidence: 99%

Measurement of radon and xenon binding to a cryptophane molecular host

Jacobson

Khan²,

Collé³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Xenon and radon have many similar properties, a difference being that all 35 isotopes of radon ( 195 Rn– 229 Rn) are radioactive. Radon is a pervasive indoor air pollutant believed to cause significant incidence of lung cancer in many geographic regions, yet radon affinity for a discrete molecular species has never been determined. By comparison, the chemistry of xenon has been widely studied and applied in science and technology. Here, both noble gases were found to bind with exceptional affinity to tris-(triazole ethylamine) cryptophane, a previously unsynthesized water-soluble organic host molecule. The cryptophane–xenon association constant, K a = 42,000 ± 2,000 M -1 at 293 K, was determined by isothermal titration calorimetry. This value represents the highest measured xenon affinity for a host molecule. The partitioning of radon between air and aqueous cryptophane solutions of varying concentration was determined radiometrically to give the cryptophane–radon association constant K a = 49,000 ± 12,000 M -1 at 293 K.

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Section: Resultsmentioning

confidence: 99%

Measurement of radon and xenon binding to a cryptophane molecular host

Jacobson

Khan²,

Collé³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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“…[120] NMR of dissolved Xe has been applied to identify binding pockets of lipid transfer protein, [121] and for observation of conformational changes in maltose binding protein, [122] and chemotaxis Y protein. [123] Besides specific site affinity for native structures, protein engineering also allowed for the design of conformation-sensitive binding pockets as demonstrated with the ribose binding protein. [124] …”

Section: 129xe Cages and Hyper-cest Mrimentioning

confidence: 99%

NMR Hyperpolarization Techniques of Gases

Barskiy

Coffey

Nikolaou

et al. 2016

Chemistry A European J

155

117

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Nuclear spin polarization can be significantly increased through the process of hyperpolarization, leading to an increase in the sensitivity of nuclear magnetic resonance (NMR) experiments by 4–8 orders of magnitude. Hyperpolarized gases, unlike liquids and solids, can be more readily separated and purified from the compounds used to mediate the hyperpolarization processes. These pure hyperpolarized gases enabled many novel MRI applications including the visualization of void spaces, imaging of lung function, and remote detection. Additionally, hyperpolarized gases can be dissolved in liquids and can be used as sensitive molecular probes and reporters. This mini-review covers the fundamentals of the preparation of hyperpolarized gases and focuses on selected applications of interest to biomedicine and materials science.

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“…In solution, 129 Xe can be used to probe protein structure 17-19 and physiologically important parameters such as blood oxygenation 20. Dissolved HP 129 Xe residing in the pulmonary capillaries rapidly diffuses into the alveolar gas spaces where its resonance frequency is shifted by ∼200 ppm.…”

Section: Introductionmentioning

confidence: 99%

Continuously Infusing Hyperpolarized ¹²⁹Xe into Flowing Aqueous Solutions Using Hydrophobic Gas Exchange Membranes

et al. 2009

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Hyperpolarized (HP) 129 Xe yields high signal intensities in magnetic resonance (MR) and, through its large chemical shift range of ∼300 ppm, provides detailed information about the local chemical environment. To exploit these properties in aqueous solutions and living tissues requires the development of methods for efficiently dissolving HP 129 Xe over an extended time period. To this end, we have used commercially available gas exchange modules to continuously infuse concentrated HP 129 Xe into flowing liquids, including rat whole blood, for periods as long as one hour, and have demonstrated the feasibility of dissolved-phase MR imaging with sub-millimeter resolution within minutes. These modules, which exchange gases using hydrophobic microporous polymer membranes, are compatible with a variety of liquids and are suitable for infusing HP 129 Xe into the bloodstream in vivo. Additionally, we have developed a detailed mathematical model of the infused HP 129 Xe signal dynamics that should be useful in designing improved infusion systems that yield even higher dissolved HP 129 Xe signal intensities.

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Design of a Conformation‐Sensitive Xenon‐Binding Cavity in the Ribose‐Binding Protein

Cited by 10 publications

References 20 publications

Measurement of radon and xenon binding to a cryptophane molecular host

Measurement of radon and xenon binding to a cryptophane molecular host

NMR Hyperpolarization Techniques of Gases

Continuously Infusing Hyperpolarized ¹²⁹Xe into Flowing Aqueous Solutions Using Hydrophobic Gas Exchange Membranes

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Design of a Conformation‐Sensitive Xenon‐Binding Cavity in the Ribose‐Binding Protein

Cited by 10 publications

References 20 publications

Measurement of radon and xenon binding to a cryptophane molecular host

Measurement of radon and xenon binding to a cryptophane molecular host

NMR Hyperpolarization Techniques of Gases

Continuously Infusing Hyperpolarized 129Xe into Flowing Aqueous Solutions Using Hydrophobic Gas Exchange Membranes

Contact Info

Product

Resources

About

Continuously Infusing Hyperpolarized ¹²⁹Xe into Flowing Aqueous Solutions Using Hydrophobic Gas Exchange Membranes