Design of a Compact Disk-like Microfluidic Platform for Enzyme-Linked Immunosorbent Assay

Lai, Siyi; Wang, Shengnian; Luo, Jun; Lee, L. James; Yang, Shang Tian; Madou, Marc

doi:10.1021/ac0348322

Cited by 392 publications

(277 citation statements)

References 11 publications

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“…In the past, fluidic control included syringe pumps (11), hydrogel valves (12), gravity-driven pumps (13), evaporation-based pumps (14,15), acoustic pumps (16), gas-generationbased pumps (17,18), and centrifugal force in CD chips (19). Electrokinetic flow (20)(21)(22), thermopneumatic (23,24), pneumatic͞hydraulic (25), or mechanical (26,27) valves and pumps have a high degree of control, but require external connection lines to larger equipment for actuation.…”

mentioning

confidence: 99%

Computerized microfluidic cell culture using elastomeric channels and Braille displays

Zhu

Futai

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

373

312

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Computer-controlled microfluidics would advance many types of cellular assays and microscale tissue engineering studies wherever spatiotemporal changes in fluidics need to be defined. However, this goal has been elusive because of the limited availability of integrated, programmable pumps and valves. This paper demonstrates how a refreshable Braille display, with its grid of 320 vertically moving pins, can power integrated pumps and valves through localized deformations of channel networks within elastic silicone rubber. The resulting computerized fluidic control is able to switch among: (i) rapid and efficient mixing between streams, (ii) multiple laminar flows with minimal mixing between streams, and (iii) segmented plug-flow of immiscible fluids within the same channel architecture. The same control method is used to precisely seed cells, compartmentalize them into distinct subpopulations through channel reconfiguration, and culture each cell subpopulation for up to 3 weeks under perfusion. These reliable microscale cell cultures showed gradients of cellular behavior from C2C12 myoblasts along channel lengths, as well as differences in cell density of undifferentiated myoblasts and differentiation patterns, both programmable through different flow rates of serumcontaining media. This technology will allow future microscale tissue or cell studies to be more accessible, especially for highthroughput, complex, and long-term experiments. The microfluidic actuation method described is versatile and computer programmable, yet simple, well packaged, and portable enough for personal use.pump ͉ valve ͉ bioreactor ͉ mixer ͉ perfusion A dvanced microfluidic cellular assays (1-6) and microscale tissue engineering studies (7-10) would benefit from robust and convenient methods to computer-control accurate spatiotemporal patterns of microfluidic flows in arrays of fluidic networks. In the past, fluidic control included syringe pumps (11), hydrogel valves (12), gravity-driven pumps (13), evaporation-based pumps (14, 15), acoustic pumps (16), gas-generationbased pumps (17, 18), and centrifugal force in CD chips (19). Electrokinetic flow (20-22), thermopneumatic (23, 24), pneumatic͞hydraulic (25), or mechanical (26, 27) valves and pumps have a high degree of control, but require external connection lines to larger equipment for actuation. A few fully integrated and self-packaged systems (23, 28) have been developed, but lack the reconfigurability inherent with numerous active valves and pumps.Here, we report a method to precisely control fluid flow inside elastomeric capillary networks by using multiple (tens to hundreds) computer-controlled, piezoelectric, movable pins. These pins are positioned as a grid on a refreshable Braille display (e.g., F. H. Papenmeier, Schwerte, Germany), which is a tactile device used by the visually impaired to read computer text. Each pin can act as a valve and be shifted upward to push against channels contained in silicone rubber and completely shut the channel. Three sequential valves of this...

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mentioning

confidence: 99%

Computerized microfluidic cell culture using elastomeric channels and Braille displays

Zhu

Futai

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

373

312

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“…The capillary forces are utilized to control the flow sequence of different reaction [21,22]. The pumping pressure of fluid generated by centrifugal force is expressed as Eq.…”

Section: Capillary Valvementioning

confidence: 99%

Centrifugal Microfluidic Integrated Optical System for Sensing Pesticide Residues

Liu¹,

Song²,

Wang³

et al. 2017

Biosens J

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We proposed and demonstrated an effective and non-destructive centrifugal microfluidic sensor (CMS) for analysis of organophosphorus pesticides (OPPs). The CMS was designed to have a sequential sample injection with two capillary valves. The actuation mechanism of CMS was controlled by centrifugal force, i.e. relative centrifugal force (×g). The OPPs analyte in CMS could be successfully derived into a mixer at 34 g and then spined into a detection reservoir at 537 g. The experiment results indicated that limit of detection for OPPs using CMS was 0.05 ppm, a linear relationship between OPPs logarithmic concentration (ppm) and inhibition rate (%) was 0.9215. Furthermore, series fresh vegetables were extracted and assayed for OPPs residue detection by using CMS. It was effective for OPPs detection. A non-destructive, rapid, reliable, and sensitive approach to OPPs analysis was demonstrated. This in turn opens up opportunities for healthcare applications, notably environmental monitoring, foods safety and other fields.

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“…For example, capillary burst valves prevent the circulation of the fluid forming a barrier when the channel section expands abruptly. Sandwich, direct and competitive immunoassays have been developed on CDs [153,154,155]. This makes these devices perfect candidates to be used in the field.…”

Section: Miniaturisation Of Immunosensorsmentioning

confidence: 99%

Trends and Perspectives in Immunosensors

2012

Antibodies Applications and New Developments

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Immunosensors are devices that comprise both a biomolecular recognition system, such as an antibody and its corresponding antigen, and a transducer to translate the high affinity and specific binding event into a physical signal.Antibodies are produced by an immunological response to the presence of a foreign substance called an antigen. Antibodies may be immobilised onto a variety of platforms including bulk planar surfaces and nanoparticles by either covalent or adsorption strategies. Different interfaces between the bio-components and the detector are available to monitor in 'real-time' the signal generated by biological interactions. The transducers detect, for example, the change in electron transfer, absorbance, fluorescence, refractive index, mass change or heat transfer as the antibody binds to the antigen of interest. Such analytical devices have allowed a wide range of analytes to be identified and quantified such as pathogens, toxins, environmental food contaminants and disease biomarkers.The demand for sensitive, rapid, 'on-site' techniques has taken advantage of the latest advances in microfluidics and nanotechnology. This chapter will highlight current trends in immobilisation, micro/nano-fluidics/ and transducers utilised. A number of examples outlining the exploitation of these elements in immunosensors and their successful applications will be described.

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Design of a Compact Disk-like Microfluidic Platform for Enzyme-Linked Immunosorbent Assay

Cited by 392 publications

References 11 publications

Computerized microfluidic cell culture using elastomeric channels and Braille displays

Computerized microfluidic cell culture using elastomeric channels and Braille displays

Centrifugal Microfluidic Integrated Optical System for Sensing Pesticide Residues

Trends and Perspectives in Immunosensors

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