Design of a biodegradable UV-irradiated gelatin-chitosan/nanocomposed membrane with osteogenic ability for application in bone regeneration

Acevedo, Cristián; Olguín, Yusser; Briceño, Mercedes; Forero, Juan Carlos; Osses, Nelson; Díaz-Calderón, Paulo; Jaques, A.; Ortiz, Rina

doi:10.1016/j.msec.2019.01.135

Cited by 37 publications

(30 citation statements)

References 101 publications

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“…Glutaraldahyde may also be a reason for reduced cell viability, as cell metabolic activity was significantly highest on Bio-Gide ® membranes at all sample points. UV-irradiation for cross-linking might present a more compatible solution (30). On RESODONT ® , cell viability was significantly higher than on the other membranes.…”

Section: Discussionmentioning

confidence: 96%

“…Furthermore, in collagenous membranes high costs and weak tensile strength in wet conditions show limitations for wide clinical application, resulting in testing of other materials such as silk (16). Lately, coated membranes including magnesium and chitosan or gelatin-chitosan nano-composed membranes have proven biocompatibility, osteogenicconductive potential, and low biodegradation rates (30,33). These new membranes might present excellent alternatives for GBR.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Biocompatibility of Different Membrane Surfaces Using Unrestricted Somatic Stem Cells

Schorn

Handschel

Lommen

et al. 2019

In Vivo

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Background/Aim: Results of Guided BoneRegeneration (GBR) primarily depend on the membrane used. The aim of this study was to compare biocompatibility of different absorbable and non-absorbable membranes by using unrestricted somatic stem cells (USSCs) as an indicator for biocompatibility. Materials and Methods: Five absorbable membranes (Bio-Gide ® , RESODONT ® , GENTA-FOIL resorb ® , BioMend ® and BioMend ® Extend™) and one non-absorbable alternative (GORE-TEX ® ) were colonized with USSCs. After 24 h, 3 days and 7 days, cell proliferation, cell viability, and cytotoxicity were assessed. Moreover, cell morphology was evaluated by electron microscopy. Results: Significantly higher cell proliferation and cell viability rates were observed in Bio-Gide ® and RESODONT ® membranes. Cell toxicity was highest on GENTA-FOIL resorb ® membranes. The electron microscopical assessment showed a better cell attachment on porous surfaced membranes. Conclusion: This study shows that USSCs can be used for assessments of biocompatibility, and that absorbable membranes with collagenous composition and porous structure tend to positively impact biocompatibility and enhance cell proliferation.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Evaluation of Biocompatibility of Different Membrane Surfaces Using Unrestricted Somatic Stem Cells

Schorn

Handschel

Lommen

et al. 2019

In Vivo

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“…The synthesis of the membranes was prepared by cold casting, using a method previously reported [20]. Briefly, the chitosan (2%) and nanoparticles were dispersed in acetic acid (1%) (titanium oxide and hydroxyapatite nanoparticles, 500 ppm).…”

Section: Membrane Synthesis and Characterizationmentioning

confidence: 99%

Osteoconductive Effect of a Nanocomposite Membrane Treated with UV Radiation

et al. 2022

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Modulation of the bio-regenerative characteristics of materials is an indispensable requirement in tissue engineering. Particularly, in bone tissue engineering, the promotion of the osteoconductive phenomenon determines the elemental property of a material be used therapeutically. In addition to the chemical qualities of the constituent materials, the three-dimensional surface structure plays a fundamental role that various methods are expected to modulate in a number of ways, one most promising of which is the use of different types of radiation. In the present manuscript, we demonstrate in a calvarial defect model, that treatment with ultraviolet irradiation allows modification of the osteoconductive characteristics in a biomaterial formed by gelatin and chitosan, together with the inclusion of hydroxyapatite and titanium oxide nanoparticles.

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“…The spreading of the cells cultured onto the microstructured films was assessed by means of image analysis using epifluorescence microscopy and ImageJ software v.1.52 (NIH, Bethesda, MD, USA). The area of the cells in each image was calculated as the size of the red stain emitted for rhodamine-phalloidin staining [26]. At least 10 images were analyzed per sample.…”

Section: Spreading Assaymentioning

confidence: 99%

A New Edible Film to Produce In Vitro Meat

Orellana

Sánchez

Benavente

et al. 2020

Foods

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In vitro meat is a novel concept of food science and biotechnology. Methods to produce in vitro meat employ muscle cells cultivated on a scaffold in a serum-free medium using a bioreactor. The microstructure of the scaffold is a key factor, because muscle cells must be oriented to generate parallel alignments of fibers. This work aimed to develop a new scaffold (microstructured film) to grow muscle fibers. The microstructured edible films were made using micromolding technology. A micromold was tailor-made using a laser cutting machine to obtain parallel fibers with a diameter in the range of 70–90 µm. Edible films were made by means of solvent casting using non-mammalian biopolymers. Myoblasts were cultured on flat and microstructured films at three cell densities. Cells on the microstructured films grew with a muscle fiber morphology, but in the case of using the flat film, they only produced unorganized cell proliferation. Myogenic markers were assessed using quantitative polymerase chain reaction. After 14 days, the expression of desmin, myogenin, and myosin heavy chain were significantly higher in microstructured films compared to the flat films. The formation of fiber morphology and the high expression of myogenic markers indicated that a microstructured edible film can be used for the production of in vitro meat.

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Design of a biodegradable UV-irradiated gelatin-chitosan/nanocomposed membrane with osteogenic ability for application in bone regeneration

Cited by 37 publications

References 101 publications

Evaluation of Biocompatibility of Different Membrane Surfaces Using Unrestricted Somatic Stem Cells

Evaluation of Biocompatibility of Different Membrane Surfaces Using Unrestricted Somatic Stem Cells

Osteoconductive Effect of a Nanocomposite Membrane Treated with UV Radiation

A New Edible Film to Produce In Vitro Meat

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