Design of a binuclear Ni(II)–iminodiacetic acid (IDA) complex for selective recognition and covalent labeling of His-tag fused proteins

Takahira, Ikuko; Fuchida, Hirokazu; Tabata, Satoshi; Shindo, Naoya; Uchinomiya, Shohei; Hamachi, Itaru; Ojida, Akio

doi:10.1016/j.bmcl.2014.04.096

Cited by 17 publications

(12 citation statements)

References 22 publications

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“…3A). If the C-terminal 6×His-tag of the connector is facing the trans-chamber, the electron donor groups on histidine imidazole rings will readily form coordinate bonds with the Ni-NTA complex on nanogold (K D of 390 nM) [37; 38]. …”

Section: Resultsmentioning

confidence: 99%

Oriented single directional insertion of nanochannel of bacteriophage SPP1 DNA packaging motor into lipid bilayer via polar hydrophobicity

Zhou

Wang

et al. 2016

Biomaterials

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Insertion of biological nanopore into artificial membrane is of fundamental importance in nanotechnology. Many applications require control and knowledge of channel orientation. In this work, the insertion orientation of the bacteriophage SPP1 and phi29 DNA packaging motor into lipid membranes was investigated. Single molecule electrophysiological assays and Ni-NTA-nanogold binding assays revealed that both SPP1 and phi29 motor channels exhibited a one-way traffic property for TAT peptide translocation from N- to C-termini of the protein channels. SPP1 motor channels preferentially inserts into liposomes with their C-terminal wider region facing inward. Changing the hydrophobicity of the N- or C-termini of phi29 connector alters the insertion orientation, suggesting that the hydrophobicity and hydrophilicity of the termini of the protein channel governs the orientation of the insertion into lipid membrane. It is proposed that the specificity in motor channel orientation is a result of the hydrophilic/hydrophobic interaction at the air/water interface when the protein channels are incorporating into liposome membranes.

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Section: Resultsmentioning

confidence: 99%

Oriented single directional insertion of nanochannel of bacteriophage SPP1 DNA packaging motor into lipid bilayer via polar hydrophobicity

Zhou

Wang

et al. 2016

Biomaterials

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“…You and co-workers also studied portions of brain tissue (hypothalamus, hippocampus, cortex, and amygdala). They found that the ratio of 31 P/ 66 Zn decreased beyond tumor limits, indicating that variations in this ratio can be used to distinguish healthy vs. cancerous tissue [14].…”

Section: Significance Development and Challenges In The Detection Omentioning

confidence: 99%

“…Aside from these interferences, the sensing results were influenced by the other factors such as the environment of the system including solvent used, type and pH of the buffer, the concentration of Zn 2+ ions present compared to the other ions, solubility properties, and of course, the characteristic and behavior of the probe itself. They are so many receptors used as fluorophore as binding sites such as di-2picolylamine (DPA) [55,56] quinoline [57,58], bipyridine [59][60][61][62], acyclic and cyclic polyamines [63,64], iminodiacetic acid [65,66], triazole [67][68][69], and Schiff-base receptors [70] ( Figure 1). However, it is necessary to notice that few compounds fluoresce by itself or fluoresce after the interaction with the target.…”

Section: Quinoline and Derivatives For Zn 2+ Fluorophoresmentioning

confidence: 99%

The Role of 8-Amidoquinoline Derivatives as Fluorescent Probes for Zinc Ion Determination

Mohamad

Zakaria

Daud

et al. 2021

Sensors

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Mass-spectrometry-based and X-ray fluorescence-based techniques have allowed the study of the distribution of Zn2+ ions at extracellular and intracellular levels over the past few years. However, there are some issues during purification steps, sample preparation, suitability for quantification, and the instruments’ availability. Therefore, work on fluorescent sensors based on 8-aminoquinoline as tools to detect Zn2+ ions in environmental and biological applications has been popular. Introducing various carboxamide groups into an 8-aminoquinoline molecule to create 8-amidoquinoline derivatives to improve water solubility and cell membrane permeability is also a recent trend. This review aims to present a general overview of the fluorophore 8-aminoquinoline and its derivatives as Zn2+ receptors for zinc sensor probes. Various fluorescent chemosensor designs based on 8-amidoquinoline and their effectiveness and potential as a recognition probe for zinc analysis were discussed. Based on this review, it can be concluded that derivatives of 8-amidoquinoline have vast potential as functional receptors for zinc ions primarily because of their fast reactivity, good selectivity, and bio-compatibility, especially for biological applications. To better understand the Zn2+ ion fluorophores’ function, diversity of the coordination complex and geometries need further studies. This review provides information in elucidating, designing, and exploring new 8-amidoquinoline derivatives for future studies for the improvement of chemosensors that are selective and sensitive to Zn2+.

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“…Immobilized metal ion affinity chromatography (IMAC) is an ideal technique for immobilizing and purifying proteins with poly-histidine tags. [9][10][11] IMAC immobilized transition-metal ions (Cu 2+ , Ni 2+ , Co 2+ , Zn 2+ ) via ligands such as one tridentate iminodiacetic acid (IDA), [12][13][14] two tetradentates nitrilotriacetic acid (NTA), [15][16][17] carboxymethylated aspartic acid (CM-Asp), 18,19 pentadentate tris (carboxymethyl) ethylenediamine (TED). 20,21 Specic interactions between transition-metal ions and polyhistidine tags (his-tags) on the N-or C-terminals of proteins facilitate protein separation and purication under different ionic strength and pH of solutions.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of magnetic nanoparticles with an IDA or TED modified surface for purification and immobilization of poly-histidine tagged proteins

et al. 2020

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Design of a binuclear Ni(II)–iminodiacetic acid (IDA) complex for selective recognition and covalent labeling of His-tag fused proteins

Cited by 17 publications

References 22 publications

Oriented single directional insertion of nanochannel of bacteriophage SPP1 DNA packaging motor into lipid bilayer via polar hydrophobicity

Oriented single directional insertion of nanochannel of bacteriophage SPP1 DNA packaging motor into lipid bilayer via polar hydrophobicity

The Role of 8-Amidoquinoline Derivatives as Fluorescent Probes for Zinc Ion Determination

Synthesis of magnetic nanoparticles with an IDA or TED modified surface for purification and immobilization of poly-histidine tagged proteins

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