Design, Formulation and Evaluation of Liposome Containing Isoniazid

Abstract

Objective: The objective of the present study was to formulate and evaluate liposomes loaded with isoniazid.Methods: Liposome of isoniazid was made by thin layer film hydration method. L-α-phosphatidylcholine and cholesterol were used to make multiamellar vesicles. Six batches of liposomes were prepared based on the different weight ratio of L-α-phosphatidylcholine and cholesterol. Differential scanning calorimetry (DSC) study conducted to study in any incompatibility.Results: The prepared liposomes were evaluated by particle size analysis, entrapment efficiency, release study and stability study. Particle sizes were determined from the scanning electron microscopy (SEM) photographs. When particle frequencies were plotted against particle diameter in the histogram, it showed that F1 batch had a skewed distribution towards smaller liposomes while F6 shows a proper bell-shaped curve with a mean at 225 mm. The percentage entrapment efficiency was found to be 8.99 ± 0.15 to 4.19 ± 0.12 % respectively. From the release profile, it was seen that F1 batch was fastest and F6 was slowest to release the drug. The satisfactory batch F1 was packed in Eppendorf tube and stored at 4 °C temperature for one month. At the end of one month, the samples were analyzed for their physical properties, drug entrapment and in vitro release profile. The percentage release was found to be 96.5 ± 3.2 after 4 h.Conclusion: The F1 batch showed most promising results compared to other. No significant change was found during one month’s stability study of final batch (F1).

Multifloral white honey outclasses manuka honey in methylglyoxal content: assessment of free and encapsulated methylglyoxal and anti-microbial peptides in liposomal formulation against toxigenic potential of Bacillus subtilis Subsp spizizenii strain

“…The encapsulation efficiency of the liposomes was calculated as: EE % = [(Amount of drug entrapped in liposome/Total amount of drug taken initially) × 100]. 27…”

Soy Lecithin-Derived Liposomal Delivery Systems: Surface Modification and Current Applications

“…One method is loading liposomes with isoniazid. According to a study by Roy et al, liposomes of isoniazid, which are made by thin-layer film hydration, showed entrapment efficiency, which was determined by the particle sizes viewed from scanning electron microscopy (SEM) photographs [63]. The process involved the use of isoniazid or soya lecithin (L-a-phosphatidylcholine) and chloroform.…”

“…Furthermore, when stored at low temperatures and light preservation, liposomes can avoid the reactions. Moreover, isoniazid liposomes were associated with proper entrapment and stability due to pH adjustments, temperature, ionic interaction, and application of cholesterol in the bilayer structure [63]. Therefore, loading liposomes with isoniazid can be used to solve entrapment issues with liposomes.…”

Selection of the Composition of a Liposomal Dosage Form of a Russian Somatostatin Analogue With Antitumor Activity

“…To produce cyphetrylin liposomes, we used the Bangham method [15]. Cyphetrylin substance and lipid components (SPC, cholesterol, and PEG-DSPE) were dissolved in chloroform.…”