Design, Fabrication, In-vitro, and Ex-Vivo Permeation Study Nisoldipine (NSP) Loaded SLNs by Modified Solvent Diffusion Method

Abstract

Objective: To deliver Nisoldipine (Nsp) transdermally, new particulate carriers have been prepared, such as stable solid lipid nanoparticles (SLNs) and nanostructured lipid carriers as gel form, using a two-factor, three-level central composite design (CCD). Method: For this experiment, The Nsp-SLNs gel was prepared using carbopol 940.We fabricated SLNs with stearic acid and tween 80 using a modified solvent diffusion method. Results: Particle size, zeta potential, polydispersity index, and in-vitro dissolution studies of the prepared nanoparticles were evaluated for their optimal use. Rat abdominal skin was also investigated for percutaneous permeation of Nsp-SLNs. Analyzing the particle size by photon correlation spectroscopy (PCS)using Malvern Zetasizer, which shows that the Nsp-SLNs are in the range of 75.49±0.63nm to 106.41±0.63nm. The entrapment efficiency (EE%) among all 9 Nsp-SLN formulations fell around 84.14±0.5% and 86.14±0.25%. Conclusion: An in-vitro drug release test was conducted over a period of 12 hours. Formulation F4 showed the optimum result based on the response surface methodology. Nsp-SLNs and Nsp-SLNsgels were successfully formulated using stearic acid and tween 80 was subjected to transdermal use. Nsp-SLNs feature a steady zeta potential window with a monodispersing range, a uniform particle size distribution within the nanoparticle range, and good encapsulation effectiveness. Higuchi and zero-order kinetics were used to predict the in-vitro release of Nsp-SLNs and gels supplemented with stearic acid and tween 80.