Design, Fabrication, and Characterization of Novel Porous Conductive Scaffolds for Nerve Tissue Engineering

Baniasadi, Hossein; Ramazani, Ahmad; Mashayekhan, Shohreh; Farani, Marzieh Ramezani; Ghaderinezhad, Fariba; Dabaghi, Mohammadhossein

doi:10.1080/00914037.2015.1038817

Cited by 29 publications

(16 citation statements)

References 31 publications

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“…After establishment of the bacterial suspension (100 L) on an agar plate, it was incubated at 37°C overnight. The remaining bacterial colonies were ultimately counted via optical microscopy 40,41…”

Section: Methodsmentioning

confidence: 99%

<p>Extending the application of a magnetic PEG three-part drug release device on a graphene substrate for the removal of Gram-positive and Gram-negative bacteria and cancerous and pathologic cells</p>

Farani

Parsi

Riazi

et al. 2019

DDDT

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Objective In this study, novel graphene oxide (GO)-based nanocomposites are presented. In fact, we have tried to replace the carboxyl groups on the surface of GO with amine groups to allow the biocompatible poly(ethylene glycol) bis(carboxymethyl) ether (average Mn 600) polymer to bond through an amide bond. Materials and methods The synthesis was conducted accurately according to final characterization experiments (Raman, X-ray diffraction [XRD], atomic force microscopy [AFM], X-ray photoelectron spectroscopy [XPS], thermogravimetric analysis [TGA], etc). The antimicrobial property of this nanocomposite was examined in Escherichia coli (ATCC 25922) as Gram-negative and Staphylococcus aureus (ATCC 25923) as Gram-positive bacterial species. Besides, curcumin (CUR) was added to the produced nanocomposite both as a promising anticancer drug and an antioxidant, the toxicity of which was then assessed on cellular-based HepG2 and pC12. Results An intense increase in toxicity was detected by MTT assay. Conclusion It can mainly be concluded that the nanocomposite synthesized in this study is capable of delivering drugs with antibacterial properties.

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Section: Methodsmentioning

confidence: 99%

<p>Extending the application of a magnetic PEG three-part drug release device on a graphene substrate for the removal of Gram-positive and Gram-negative bacteria and cancerous and pathologic cells</p>

Farani

Parsi

Riazi

et al. 2019

DDDT

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“…However, its poor mechanical stability limited its applications, and therefore, modification of its mechanical properties is inevitable. [27][28][29] It is verified that the product of periodate oxidation (oxidized alginate, OA) is a better crosslinking agent for gelatin than natural alginate due to the reaction between available aldehyde groups of OA and free amino groups of lysine or hydroxylysine amino acid residues of gelatin. 11,15 Moreover, periodate oxidation of alginate would promote its hydrolysis in aqueous solutions.…”

Section: Introductionmentioning

confidence: 99%

Design, fabrication and characterization of oxidized alginate–gelatin hydrogels for muscle tissue engineering applications

et al. 2016

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In this study, we reported the preparation of self cross-linked oxidized alginate-gelatin hydrogels for muscle tissue engineering. The effect of oxidation degree (OD) and oxidized alginate/gelatin (OA/GEL) weight ratio were examined and the results showed that in the constant OA/GEL weight ratio, both cross-linking density and Young's modulus enhanced by increasing OD due to increment of aldehyde groups. Furthermore, the degradation rate was increased with increasing OD probably due to decrement in alginate molecular weight during oxidation reaction facilitated degradation of alginate chains. MTT cytotoxicity assays performed on Wharton's Jelly-derived umbilical cord mesenchymal stem cells cultured on hydrogels with OD of 30% showed that the highest rate of cell proliferation belong to hydrogel with OA/GEL weight ratio of 30/70. Overall, it can be concluded from all obtained results that the prepared hydrogel with OA/GEL weight ratio and OD of 30/70 and 30%, respectively, could be proper candidate for use in muscle tissue engineering.

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“…Gelatin is a well-known natural biopolymer with cell binding sites, 10 which support cell attachment and proliferation as investigated in several studies. 10,11 Therefore, the scaffold fabricated with pure gelatin support attachment and proliferation of MSCs as confirmed via applied MTT assay test as illustrated in Figure 8(a). The presence of PAG just slightly reduced the cell viability during the test period, therefore, it can be concluded that the optimum scaffolds with PAG have supported the proliferation of MSCs during the test period.…”

Section: Characterization Of Optimum Scaffoldmentioning

confidence: 83%

“…10 Since gelatin is hydrophilic natural polymer and our synthesized PAG nanocomposite can easily disperse in the aqueous solution, 15 its proper dispersion and interaction with the gelatin matrix is acceptable. In several studies, 10,11 the proper range for the tensile modulus for nerve tissue engineering scaffolds has been reported in the range of 1-10 kPa, therefore our optimum fibrous scaffold could be considered as a proper candidate for nerve tissue engineering application.…”

Section: Characterization Of Optimum Scaffoldmentioning

confidence: 91%

“…Blends of chitosan and gelatin have been previously reported to be effective for nerve, skin, cartilage, bone, and muscle tissue engineering. 10,11 In recent years, conducting polymeric substrates have received considerable attention as a suitable material in neural tissue engineering, since bioelectricity plays an integral role in maintaining biological functions such as signaling of the nervous system and electrical stimulation is an effective cue for stimulating cell proliferation and differentiations. 12 In our previous work, 10 we successfully synthesized conductive polyaniline/graphene nanocomposite (PAG) and used it for fabrication of conductive chitosan/gelatin porous scaffold for nerve tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

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Design and fabrication of conductive nanofibrous scaffolds for neural tissue engineering: Process modeling via response surface methodology

et al. 2018

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Peripheral nervous system in contrary to central one has the potential for regeneration, but its regrowth requires proper environmental conditions and supporting growth factors. The aim of this study is to design and fabricate a conductive polyaniline/graphene nanoparticles incorporated gelatin nanofibrous scaffolds suitable for peripheral nervous system regeneration. The scaffolds were fabricated with electrospinning and the fabrication process was designed with Design-Expert software via response surface methodology. The effect of process parameters including applied voltage (kV), syringe pump flow rate (cm 3 /h), and PAG concentration (wt%), on the scaffold conductivity, nanofibers diameter, and cell viability were investigated. The obtained results showed that the scaffold conductivity and cell viability are affected by polyaniline/graphene concentration while nanofiber diameter is more affected by the applied voltage and syringe pump flow rate. Optimum scaffold with maximum conductivity (0.031 AE 0.0013 S/cm) and cell compatibility and suitable diameter were electrospun according to the software introduced values for the process parameters (voltage of 13 kV, flow rate of 0.1 cm 3 /h, and PAG wt.% of 1.3) and its morphology, cell compatibility, and biodegradability were further investigated, which showed its potential for applying in peripheral nervous system injury regeneration.

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Design, Fabrication, and Characterization of Novel Porous Conductive Scaffolds for Nerve Tissue Engineering

Cited by 29 publications

References 31 publications

<p>Extending the application of a magnetic PEG three-part drug release device on a graphene substrate for the removal of Gram-positive and Gram-negative bacteria and cancerous and pathologic cells</p>

<p>Extending the application of a magnetic PEG three-part drug release device on a graphene substrate for the removal of Gram-positive and Gram-negative bacteria and cancerous and pathologic cells</p>

Design, fabrication and characterization of oxidized alginate–gelatin hydrogels for muscle tissue engineering applications

Design and fabrication of conductive nanofibrous scaffolds for neural tissue engineering: Process modeling via response surface methodology

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