Design, development and optimization of new high performance thin‐layer chromatography method for quantitation of Retapamulin in pharmaceutical formulation: Application of design of experiment

Abstract

The objective of the present study was to design, develop and optimize Design of Experiments supported high performance thin-layer chromatography method for quantitative estimation of Retapamulin in marketed cream and microemulsion formulations. Design of Experiments help to identify and understand the effect of important factors on response with minimum experimental run in developing robust method. To optimize chromatographic conditions and to study their effects on separation of Retapamulin, Box-Behnken experimental design was selected. Experimental conditions with desirability 1 were selected which gave the optimal composition of methanol in mobile phase along with ethyl acetate: ammonia, development distance of 70 millimeter and chamber saturation time of 22 minutes. Retapamulin was well separated with retardation factor value 0.60 from excipients of marketed cream formulation on high performance thin-layer chromatography plate using optimized chromatographic conditions. The optimized method was validated and statistical analysis proved that the method is specific, sensitive, accurate and precise. A new high performance thin-layer chromatography has been successfully designed, developed, optimized and validated for quantification of Retapamulin in cream formulation using Box-Behnken design.The proposed high performance thin-layer chromatography method is easy, convenient, reliable and accurate for the routine analysis of Retapamulin in marketed cream and microemulsion formulations.

Simultaneous estimation of mometasone furoate and olopatadine hydrochloride by reversed‐phase high‐performance liquid chromatography

“…As per the literature review, a specific HPTLC method Validated for estimation of MOM and OLO methods is available [51].…”

Green and sensitive detection of olopatadine in aqueous humor using a signal‐on fluorimetric approach: GREEnness assessment

“…This drug comes in the form of eye drops and is commonly prescribed to relieve symptoms such as itching, redness, and watering of the eyes caused by allergies like hay fever or allergic conjunctivitis [20][21][22]. Most of the detection approaches for OLP are chromatographic approaches [23][24][25][26][27][28][29][30][31][32][33]. While these methods provide outstanding detection limits and the capability to analyze OLP degradation by-products, their use is constrained by timeintensive procedures, extended pretreatment steps of the designated compounds or drug, and the need for exceptionally highpurity solvents, along with a requirement for skilled personnel.…”

Simultaneous quantification of Fluticasone propionate and Olopatadine hydrochloride by High‐performance thin‐layer chromatography

“…Olopatadine is official in Indian Pharmacopoeia [2] and US Pharmacopoeia [3]. From the literature review, it is observed that many analytical methods have been developed involving spectrophotometric [4–14], LC [14], HPLC [12, 15–17], voltammetry [20], HPTLC [15, 18–19, 22], stability‐indicating HPLC method [17], and LC‐ESI‐MS‐MS[21] for the estimation of OLO in pharmaceutical formulations. Fluticasone propionate (FLU) Figure 1, chemically [(6 S ,8 S ,9 R ,10 S ,11 S ,13 S ,14 S ,16 R ,17 R )−6,9‐difluoro‐17‐(fluoromethylsulfanyl‐carbonyl)−11‐hydroxy‐10,13,16‐trimethyl‐3‐oxo‐6,7,8,11,12,14,15,16‐octahydrocyclopenta‐[a]phenanthren‐17‐yl] propanoate Trifluorinated Gluco‐corticoid receptor with anti‐allergic and anti‐inflammatory effect [23].…”

“…official in Indian Pharmacopoeia [2] and US Pharmacopoeia [3]. From the literature review, it is observed that many analytical methods have been developed involving spectrophotometric [4][5][6][7][8][9][10][11][12][13][14], LC [14], HPLC [12,[15][16][17], voltammetry [20], HPTLC [15,[18][19]22], stabilityindicating HPLC method [17], and LC-ESI-MS-MS [21] for the estimation of OLO in pharmaceutical formulations. Fluticasone propionate (FLU) Figure 1 thren-17-yl] propanoate Trifluorinated Gluco-corticoid receptor with anti-allergic and anti-inflammatory effect [23].…”