The memristive crossbar Physical Unclonable Function (PUF) structures are emerging as strong security primitives for resource-constrained devices demanding good retention time, negligible standby power, small size, and ultra-low power operating requirements. Memristive PUFs exploit the inherent high process variations of a memristor as a source of entropy to generate device-specific signatures. These PUFs need to be strong enough to deal with active and passive attacks as well as machine learning attacks. This requires more variability in the device characteristics. Memristive PUF requires dense crossbar architecture to generate unique, uniform, and reliable device signatures. Dense memristive crossbars (Xbar) face the challenge of low noise margin, proper load resistance selection, scalability, and a precise sense circuitry at the load resistance side to read the resistive states of a memristor accurately. In this work, we have simulated and optimized the load resistance of memristive scaled up crossbar arrays. We have used two of our fabricated devices for memristive crossbar PUF simulation. The proposed crossbar PUF architecture satisfies the basic PUF evaluation metrics and improves noise margin (NM). The load resistance is optimized through MATLAB simulation. The impact of optimized load resistance on Xbar architecture is observed to be noticeable and around 18% improvement in the noise margin was observed when the crossbar is scaled up from 16×2 to 128×2.