Design and Synthesis of Triphenylphosphonium Functionalized Nanoparticle Probe for Mitochondria Targeting and Imaging

Chakraborty, Atanu; Jana, Nikhil R.

doi:10.1021/jp511870e

Cited by 67 publications

(85 citation statements)

References 52 publications

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“…This result is consistent with previously published data 16, 39, 46 . Bielski et al found that conjugating TPP onto poly(amidoamine) (PAMAM) dendrimer nanocarriers increased both cellular internalization and mitochondrial targeting in the human lung adenocarcinoma cell line, A549 16 .…”

Section: Resultssupporting

confidence: 94%

“…Two assays were performed to confirm TPP-particle conjugation: 1) zeta potential measurements and 2) detection of residual primary amines with fluorescamine. Since TPP is a lipophilic cation its successful conjugation onto the particle surface results in an increase in zeta potential 39 . As shown in Figure 3, TPP-PLGA:PLGA-NH 2 , 50:50 particles demonstrated significantly higher zeta potential when compared to unmodified particles (PLGA:PLGA-NH 2 , 50:50).…”

Section: Resultsmentioning

confidence: 99%

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Surface-modified particles loaded with CaMKII inhibitor protect cardiac cells against mitochondrial injury

Wongrakpanich

Morris

Geary

et al. 2017

International Journal of Pharmaceutics

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An excess of calcium (Ca2+) influx into mitochondria during mitochondrial re-energization is one of the causes of myocardial cell death during ischemic/reperfusion injury. This overload of Ca2+ triggers the mitochondrial permeability transition pore (mPTP) opening which leads to programmed cell death. During the ischemic/reperfusion stage, the activated Ca2+/calmodulin-dependent protein kinase II (CaMKII) enzyme is responsible for Ca2+ influx. To reduce CaMKII-related cell death, sub-micron particles composed of poly(lactic-co-glycolic acid) (PLGA), loaded with a CaMKII inhibitor peptide were fabricated. The CaMKII inhibitor peptide-loaded (CIP) particles were coated with a mitochondria targeting moiety, triphenylphosphonium cation (TPP), which allowed the particles to accumulate and release the peptide inside mitochondria to inhibit CaMKII activity. The fluorescently labeled TPP-CIP were taken up by mitochondria and successfully reduced ROS caused by Isoprenaline (ISO) in a differentiated rat cardiomyocyte-like cell line. When cells were treated with TPP-CIP prior ISO exposure, they maintained mitochondrial membrane potential. The TPP-CIP protected cells from ISO-induced ROS production and decreased mitochondrial membrane potential. Thus, TPP-CIP have the potential to be used in protection against ischemia/reperfusion injury.

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Section: Resultssupporting

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Surface-modified particles loaded with CaMKII inhibitor protect cardiac cells against mitochondrial injury

Wongrakpanich

Morris

Geary

et al. 2017

International Journal of Pharmaceutics

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“…Mallick et al developed this approach for dual drug delivery using α‐tocopheryl succinate to damage mitochondria, and cisplatin and DOX to destroy DNA in nuclei subcellular of HeLa cells . By targeting organelles, direct lethal damage to cancer cells at the genetic and metabolic levels would become more efficient …”

Section: Fine‐tuning Strategies For Better 2dnmsmentioning

confidence: 99%

Two‐Dimensional Nanomaterials for Cancer Nanotheranostics

Chen

Sun

et al. 2017

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Emerging nanotechnologies show unprecedented advantages in accelerating cancer theranostics. Among them, two-dimensional nanomaterials (2DNMs) represent a novel type of material with versatile physicochemical properties that have enabled a new horizon for applications in both cancer diagnosis and therapy. Studies have demonstrated that 2DNMs may be used in diverse aspects, including i) cancer detection due to their high propensity towards tumor markers; ii) molecular imaging for guided tumor therapies, and iii) drug and gene loading, photothermal and photodynamic cancer therapies. However, their biomedical applications raise concerns due to the limited understanding of their in vivo metabolism, transformation and possible toxicities. In this comprehensive review, the state-of-the-art development of 2DNMs and their implications for cancer nanotheranostics are presented. The modification strategies to enhance the biocompatibility of 2DNMs are also reviewed.

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“…Quantum dots (QDs) are a kind of semiconductor fluorescent semiconductors, which have gained attraction in recent years by scientists as a novel fluorescent probe [1][2][3][4][5][6]. Compared with conventional organic fluorescent probes, QDs have shown great potential to be fluorescent probes and images in biology because of their unique optical properties [7][8][9][10][11][12][13][14][15].…”

Section: Reviewmentioning

confidence: 99%

Application of functional quantum dot nanoparticles as fluorescence probes in cell labeling and tumor diagnostic imaging

Zhao¹,

Zeng²

2016

Nano Online

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Quantum dots (QDs) are a class of nanomaterials with good optical properties. Compared with organic dyes, QDs have unique photophysical properties: size-tunable light emission, improved signal brightness, resistance against photobleaching, and simultaneous excitation of multiple fluorescence colors. Possessing versatile surface chemistry and superior optical features, QDs are useful in a variety of in vitro and in vivo applications. When linked with targeting biomolecules, QDs can be used to target cell biomarkers because of high luminescence and stability. So QDs have the potential to become a novel class of fluorescent probes. This review outlines the basic properties of QDs, cell fluorescence labeling, and tumor diagnosis imaging and discusses the future directions of QD-focused bionanotechnology research in the life sciences.Keywords: Nanoparticles; Quantum dots; Optical properties; Biomaterials ReviewQuantum dots (QDs) are a kind of semiconductor fluorescent semiconductors, which have gained attraction in recent years by scientists as a novel fluorescent probe [1][2][3][4][5][6]. Compared with conventional organic fluorescent probes, QDs have shown great potential to be fluorescent probes and images in biology because of their unique optical properties [7][8][9][10][11][12][13][14][15]. Alivisatos and Nie employed QDs as fluorescent probes in biological staining and diagnostics that provided a novel study to display QDs as labels for cell and tissue research [16,17]. In recent years, QDs have been widely used in many fields of life sciences as fluorescent markers [18][19][20][21]. Herein, we briefly review the basic properties of QDs and the application of functionalized QDs as fluorescent probes in biological systems. The unique properties of quantum dotsQDs have unique optical and electrical properties due to its quantum effect and size effect. When the size of a particle is of nanometer scale, it will cause quantum confinement effect, size effect, dielectric confinement effect, macroscopic quantum effect, and surface effect. Consequently, QDs exhibit many optical properties different from macroscopic materials, and they have a very broad application prospects in biological fluorescent probes and functional materials. Therefore, QDs will have a meaningful effect on the continued development of life sciences [22][23][24][25][26][27][28]. Quantum dots have unique optical propertiesQDs have gained attraction owing to their unique fluorescence properties, which are due to the electron-hole and the interaction with the surrounding environment. When the excitation level of photon exceeds the band gap, the QDs will absorb photons to make the electrons transit from the valence band to the conduction band and shine. And if the composition and size of the QDs changed, they could obtain the emission spectra within the range from blue to red [29][30][31]. Conversely, when we want to obtain a variety of colors from multifarious fluorescent dyes, we often need a variety of excitation light, which not only incre...

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Design and Synthesis of Triphenylphosphonium Functionalized Nanoparticle Probe for Mitochondria Targeting and Imaging

Cited by 67 publications

References 52 publications

Surface-modified particles loaded with CaMKII inhibitor protect cardiac cells against mitochondrial injury

Surface-modified particles loaded with CaMKII inhibitor protect cardiac cells against mitochondrial injury

Two‐Dimensional Nanomaterials for Cancer Nanotheranostics

Application of functional quantum dot nanoparticles as fluorescence probes in cell labeling and tumor diagnostic imaging

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