Design and Synthesis of Biotinylated Troglitazone as an Active Affinity Probe

Abstract

A biotin-tagged analogue of troglitazone was designed, synthesized and applied to affinity chromatography to pulldown EGFR from 293T cell lysates overexpressing EGFR, a membrane protein assumed to be troglitazone's direct binding target by previous work. The results indicate the feasibility of the biotinylated probe as a vigorous tool to clarify the molecular mechanisms for troglitazone's antitumor activities.

Design and Analysis of Drag-bar of Planter Based on Modal Analysis

“…The traditional design of planter drag-bar is to use the analogy method and the strength check. It caused the strength of drag-bar is too large and materials waste [1].The dynamic design and analysis of the planter drag-bar with the modal analysis technique can get more accurate structure natural frequency, mode shape, modal damping, modal mass and modal stiffness. So the structure design can avoid the frequency or minimize the excitation of these frequencies, so as to eliminate the excessive vibration and ensure the reliability of the structure [2].…”

Photocatalytic Degradation of Phenol by Impure BiFeO₃ under Visible Light Irradiation

“…B i s m u t h f e r r i t e ( BiFeO 3 ) is an interesting multiferroic material for photocatalytic applications with a small band gap (2.2 eV), good carrier transport properties, large absorption of visible light extending up 750 nm and high chemical stability during the photocatalytic process [3][4][5]. Recently, many research groups have developed various methods to prepare pure BiFeO 3 including conventional solid state [6], co-precipitation [7], hydrothermal synthesis [8][9] and sol-gel techniques [10][11][12]. However, investigations carried out on the synthesis of pure phase BiFeO 3 are limited because of specific facility requirements.…”