Design and Production of a Chimeric Resilin-, Elastin-, and Collagen-Like Engineered Polypeptide

Bracalello, Angelo; Santopietro, Valentina; Vassalli, Massimo; Marletta, Giovanni; Gaudio, Rosanna del; Bochicchio, Brigida; Pepe, Antonietta

doi:10.1021/bm2005388

Cited by 88 publications

(93 citation statements)

References 60 publications

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“…Other recombinant proteins are designed to mimic collagen or contain collagen-like domains: a tailored DNA sequence is designed and built and the desired protein is expressed in a host via genetic engineering. The protein sequence can be dictated to control parameters such as mechanics and cellular responses, and then optimized for tailored applications [91,95–97]. …”

Section: Biopolymer Design: Case Studies and Examplesmentioning

confidence: 99%

A review of combined experimental and computational procedures for assessing biopolymer structure–process–property relationships

et al. 2012

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Tailored biomaterials with tunable functional properties are desirable for many applications ranging from drug delivery to regenerative medicine. To improve the predictability of biopolymer materials functionality, multiple design parameters need to be considered, along with appropriate models. In this article we review the state of the art of synthesis and processing related to the design of biopolymers, with an emphasis on the integration of bottom-up computational modeling in the design process. We consider three prominent examples of well-studied biopolymer materials – elastin, silk, and collagen – and assess their hierarchical structure, intriguing functional properties and categorize existing approaches to study these materials. We find that an integrated design approach in which both experiments and computational modeling are used has rarely been applied for these materials due to difficulties in relating insights gained on different length- and time-scales. In this context, multiscale engineering offers a powerful means to accelerate the biomaterials design process for the development of tailored materials that suit the needs posed by the various applications. The combined use of experimental and computational tools has a very broad applicability not only in the field of biopolymers, but can be exploited to tailor the properties of other polymers and composite materials in general.

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Section: Biopolymer Design: Case Studies and Examplesmentioning

confidence: 99%

A review of combined experimental and computational procedures for assessing biopolymer structure–process–property relationships

et al. 2012

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“…Though it is difficult to produce a stable triple helix, synthetic collagen-like peptides derived from chemical synthesis have been investigated for their potential application as biomaterials, particularly in the areas of elastin-collagen based scaffolding [78,91], tissue engineering [92,93], and delivery [94]. The range of material properties of these hybrid materials has been extended through the synthesis of a resilin-elastin-collagen-like polypeptide (REC) [95]. The recombinant material incorporates both collagen and elastin as well as resilin, an elastomeric protein with is noted for high resilience and very high fatigue lifetime [96].…”

Section: Synthesis Of Hybrid Biomaterialsmentioning

confidence: 99%

Designing protein-based biomaterials for medical applications

2014

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Biomaterials produced by nature have been honed through billions of years, evolving exquisitely precise structure-function relationships that scientists strive to emulate. Advances in genetic engineering have facilitated extensive investigations to determine how changes in even a single peptide within a protein sequence can produce biomaterials with unique thermal, mechanical and biological properties. Elastin, a naturally occurring protein polymer, serves as a model protein to determine the relationship between specific structural elements and desirable material characteristics. The modular, repetitive nature of the protein facilitates the formation of well-defined secondary structures with the ability to self-assemble into complex three-dimensional architectures on a variety of length scales. Furthermore, many opportunities exist to incorporate other protein-based motifs and inorganic materials into recombinant protein-based materials, extending the range and usefulness of these materials in potential biomedical applications. Elastin-like polypeptides can be assembled into 3D architectures with precise control over payload encapsulation, mechanical and thermal properties, as well as unique functionalization opportunities through both genetic and enzymatic means. An overview of current protein-based materials, their properties and uses in biomedicine will be provided, with a focus on the advantages of elastin-like polypeptides. Applications of these biomaterials as imaging and therapeutic delivery agents will be discussed. Finally, broader implications and future directions of these materials as diagnostic and therapeutic systems will be explored.

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“…These could include composite structures of different bacterial collagen components, for example, using different species for the folding and collagen domains, or different collagen domains, or constructs that include bacterial collagens and different proteins, which, for example, could be silks, elastic proteins, or other collagens. 49 Opportunities also exist to further functionalize the bacterial collagen core protein to allow selective simple and complex tethering of one of more peptides or nonpeptide molecules for off the shelf development of specific protein materials with a defined application.…”

Section: Conclusion and Future Trendsmentioning

confidence: 99%