Design and Performance of a Mass-analyzed Ion Kinetic Energy (MIKE) Spectrometer

Beynon, J. H.; Cooks, R. Graham; Amy, J. W.; Baitinger, W. E.; Ridley, Thomas Y.

doi:10.1021/ac60334a763

Cited by 274 publications

(67 citation statements)

References 19 publications

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“…The E. coli tRNA that was used for these experiments is a mixture of tRNAs of which approximately two-thirds are 76 nucleotides in length and the other third are approximately 86 in length and most of the species have been sequenced. By consulting the recently published compilation of tRNA sequences by Gauss and Sprinzl8, we were able to make a list of all of the possible modified nucleosides that have been found in the E. coli tRNAs which is the following : 4-thiouridine, dihydrouridine, ribothymidine, pseudouridine, 7 inosine, and several unidentified derivatives of uridine, guanosine, and adenosine. Obviously, all of these modified nucleosides are not present in the tRNA used for this study, but the possibility of their presence had to be considered when the mass spectrum of the tRNA was examined.…”

Section: Resultsmentioning

confidence: 99%

Identification of modified nucleosides in intact transfer ribonucleic acid by pyrolysis-electron impact-collisional activation mass spectrometry

Puzo¹,

Wiebers²

1981

Nucl Acids Res

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A novel mass spectrometric method has been developed, for the detection and identification of dihydrouridine, ribothymidine, 4-thiouridine, and 7-methylguanosine in Escherichia coli tRNAs. The method utilizes (a) Pyrolysis-Electron Impact-Mass Spectrometry (PYEIMS), a procedure which releases the purine and pyrimidine bases from the intact, underivatized tRNA molecule. The mass spectrum exhibits intense peaks for the bases deriving from the comnnon nucleosides in tRNA as well as peaks of much lower intensity at mass values expected for the bases from modified components known to be present in the tRNA; and,(b) Collisional Activation Mass Spectrometry (CAMS), a technique which permits the isolation of a single ion species from a complex mass spectrum. Subsequent fragmentation of that species yields a characteristic collisional activation spectrum. Such analyses of the ion species that were presumed to originate from H2Urd, rThd, 4SUrd, and 7MeGuo in the tRNA were used to define the structure and, thus, the identity of each component. Attributes of the PYEICAMS technique are that (a) precise structural elucidation of minor nucleosides present in tRNAs at the I -4 % level is obtained; (b) the high order of sensitivity allows the analysis to be done on microgram amounts of tRNA; and (c) there is no requirement for enzymatic or chemical hydrolysis of the tRNA or for subsequent chromatographic separation methods.

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Section: Resultsmentioning

confidence: 99%

Identification of modified nucleosides in intact transfer ribonucleic acid by pyrolysis-electron impact-collisional activation mass spectrometry

Puzo¹,

Wiebers²

1981

Nucl Acids Res

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“…Data were also obtained on an Hitachi RMH-2 via high voltage scans for dissociations in the 1st FFR" and a MIKE spectrometer gave data for dissociation in the 2nd FFR by MIKE scans. 12 The data are presented in Tables 1 and 2, and Fig. 1: they cover a wide range of ion lifetimes extending from approximately 1-40 ks, the longer times being accessible only by MIKE scans with the triple sector instrument.…”

Section: Resultsmentioning

confidence: 99%

Kinetic energy release and ion structure: [C₆H₆O]⁺˙

Maquestiau

Flammang

Glish

et al. 1980

Org. Mass Spectrom.

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It is shown that, contrary to a recent report, the kinetic energy release for CO loss from phenol does not decrease as the ion accelerating voltage is decreased. In fact, an increase is observed which is attributed, at least in part, to greater discrimination against off-axis ions at low voltage. The kinetic energy release data do not demand the tautomerization of ionized phenol before decarbonylation.

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“…For all this early work, sector instruments were used exclusively and CID was performed at high kinetic energies. There was a lot of research effort focused on MS/MS sector instrumentation at this time [13][14][15][16][17][18][19][20][21][22]. However, in 1978, Yost and Enke published the first results using CID in a triple quadrupole system for MS/MS [23].…”

Section: Beam-beam Hybrid Instrumentsmentioning

confidence: 99%

Hybrid mass spectrometers for tandem mass spectrometry

Glish

Burinsky

2008

J. Am. Soc. Mass Spectrom.

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Mass spectrometers that use different types of analyzers for the first and second stages of mass analysis in tandem mass spectrometry (MS/MS) experiments are often referred to as "hybrid" mass spectrometers. The general goal in the design of a hybrid instrument is to combine different performance characteristics offered by various types of analyzers into one mass spectrometer. These performance characteristics may include mass resolving power, the ion kinetic energy for collision-induced dissociation, and speed of analysis. This paper provides a review of the development of hybrid instruments over the last 30 years for analytical applications. (J Am Soc Mass Spectrom 2008, 19, 161-172) © 2008 American Society for Mass Spectrometry T andem mass spectrometry (MS/MS), in a very generic description, is a process in which an ion formed in an ion source is mass-selected in the first stage of analysis, reacted, and then the charged products from the reaction are analyzed in the second stage of analysis. The type and quality of data that is obtained can vary greatly depending upon the type of analyzer used in the first and second stages of analysis, and the type of reaction performed between the stages of analysis. The reactions that can be done also can depend upon the type of analyzer. Over the years there have been a variety of means developed to measure the mass-to-charge ratio of gas-phase ions. The most common methods involve: dispersion based on ion momentum or kinetic energy (magnetic and electric sector instruments); separation in time based on ion velocity (time-of-flight); transmission through an electrodynamic field (quadrupole mass filter); and periodic motion in a magnetic or electrodynamic field (ion traps). There are differences in the experimental parameters associated with these various analysis methods that are pertinent to the MS/MS experiment. Some parameters are obvious, typically related to the performance of the mass analyzer while others are more subtle, related to the reactions/chemistry occurring between the stages of analysis. Many times these different parameters are used to categorize MS/MS experiments.One parameter is the ion kinetic energy. Sector and time-of-flight (TOF) instruments typically operate at "high" ion kinetic energies (5-20 keV), whereas "low" ion kinetic energies (Ͻ50 eV) are typical in quadrupole mass filters and ion traps. The ion kinetic energy is an important parameter in MS/MS experiments because the most common reaction involves colliding the ion of interest with a target gas atom or molecule. When performing ion-neutral collision experiments, the possible reactions that can be accessed (e.g., collisioninduced dissociation, collisional cooling, charge permutation, ion/molecule reaction) depends upon the ion kinetic energy. The appearance of the MS/MS spectrum can change drastically as a function of the collision (ion kinetic) energy. A related factor that is equally important, but often not considered, is the time frame of the experiment, that is, the elapsed time bet...

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Design and Performance of a Mass-analyzed Ion Kinetic Energy (MIKE) Spectrometer

Abstract: Ion kinetic energy spectrometry allows the study of both unimolecular and bimolecular reactions and is a unique source of information on thermochemistry, molecular structure, isotopic quantitation, and reaction dynamics

Cited by 274 publications

References 19 publications

Identification of modified nucleosides in intact transfer ribonucleic acid by pyrolysis-electron impact-collisional activation mass spectrometry

Identification of modified nucleosides in intact transfer ribonucleic acid by pyrolysis-electron impact-collisional activation mass spectrometry

Kinetic energy release and ion structure: [C₆H₆O]⁺˙

Hybrid mass spectrometers for tandem mass spectrometry

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Design and Performance of a Mass-analyzed Ion Kinetic Energy (MIKE) Spectrometer

Abstract: Ion kinetic energy spectrometry allows the study of both unimolecular and bimolecular reactions and is a unique source of information on thermochemistry, molecular structure, isotopic quantitation, and reaction dynamics

Cited by 274 publications

References 19 publications

Identification of modified nucleosides in intact transfer ribonucleic acid by pyrolysis-electron impact-collisional activation mass spectrometry

Identification of modified nucleosides in intact transfer ribonucleic acid by pyrolysis-electron impact-collisional activation mass spectrometry

Kinetic energy release and ion structure: [C6H6O]+˙

Hybrid mass spectrometers for tandem mass spectrometry

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Kinetic energy release and ion structure: [C₆H₆O]⁺˙